Yeast mutants blocked in removing the methyl group of lanosterol at C-14. Separation of sterols by high-pressure liquid chromatography

Biochemistry ◽  
1977 ◽  
Vol 16 (21) ◽  
pp. 4721-4726 ◽  
Author(s):  
Paul J. Trocha ◽  
Stanley J. Jasne ◽  
David B. Sprinson
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Methyl Group ◽  
Yeast Mutants

scholarly journals A tryptophan C-methyltransferase involved in streptonigrin biosynthesis in Streptomyces flocculus

1984 ◽  
Vol 220 (1) ◽  
pp. 309-313 ◽  
Author(s):  
D L Hartley ◽  
M K Speedie
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Amino Acid ◽  
Stationary Phase ◽  
High Pressure Liquid Chromatography ◽  
Absolute Configuration ◽  
Specific Activity ◽  
Shake Culture ◽  
Methyl Group ◽  
The Absolute

A C-methyltransferase that catalyses the transfer of a methyl group from S-adenosylmethionine to C-3 of tryptophan, resulting in beta-methyltryptophan, has been identified in cell-free extracts of streptonigrin-producing Streptomyces flocculus. The absolute configuration of the product was shown to be (2S,3R)-beta-methyltryptophan by high-pressure liquid chromatography and reactivity with D- and L-amino acid oxidases. In shake culture, maximum specific activity occurs after S. flocculus enters stationary phase, but before significant streptonigrin accumulates.

Historical chemical annotations of Cinchona bark collections are comparable to results from current day high-pressure liquid chromatography technologies

2020 ◽  
Vol 249 ◽  
pp. 112375 ◽  
Author(s):  
Nataly Allasi Canales ◽  
Tobias Nikolaj Gress Hansen ◽  
Claus Cornett ◽  
Kim Walker ◽  
Felix Driver ◽  
...  
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography
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