Mechanism of purine nucleoside handling and transport in isolated membrane vesicles from polyoma transformed BHK/21 cells

Biochemistry ◽  
1977 ◽  
Vol 16 (20) ◽  
pp. 4526-4532 ◽  
Author(s):  
Diana J. Dowd ◽  
Dennis C. Quinlan ◽  
Joy Hochstadt
Keyword(s):  
Membrane Vesicles ◽  
Purine Nucleoside ◽  
Isolated Membrane Vesicles

Metabolism and Transport of Purine Nucleosides by Membrane Preparations of Micrococcus sodonensis

1974 ◽  
Vol 52 (2) ◽  
pp. 83-89 ◽  
Author(s):  
M. A. Pickard ◽  
Lucille Phillippe ◽  
J. N. Campbell
Keyword(s):  
Adenosine Deaminase ◽  
Membrane Vesicles ◽  
Purine Nucleoside ◽  
Nucleoside Transport ◽  
Marked Contrast ◽  
Adenosine Concentration ◽  
Membrane Bound ◽  
Uptake And Metabolism ◽  
Isolated Membrane Vesicles ◽  
Bacterial Systems

The uptake and metabolism of adenosine-8-14C was studied using isolated membrane vesicles prepared from Micrococcus sodonensis. When the adenosine concentration was 1.0 mM, a concentration that caused prolonged bacteriostasis in growing cells, the membranes vesicles initially accumulated adenosine and inosine to similar levels but rapidly became enriched with inosine. However, when the external adenosine concentration was lowered to 0.1 mM, the vesicle contents were almost entirely inosine. No compounds other than adenosine and inosine were detected. These effects were shown to be the result of a membrane-bound adenosine deaminase and are in marked contrast to the phosphoribosyltransferase-linked mechanisms of purine nucleoside transport in other bacterial systems.

Rotavirus interaction with isolated membrane vesicles.

1994 ◽  
Vol 68 (6) ◽  
pp. 4009-4016 ◽  
Author(s):  
M C Ruiz ◽  
S R Alonso-Torre ◽  
A Charpilienne ◽  
M Vasseur ◽  
F Michelangeli ◽  
...  
Keyword(s):  
Membrane Vesicles ◽  
Isolated Membrane Vesicles

Use of Isolated Membrane Vesicles in Transport Studies

Transport ◽  
1975 ◽  
pp. 117-162 ◽  
Author(s):  
Joy Hochstadt ◽  
Dennis C. Quinlan ◽  
Richard L. Rader ◽  
Chien-Chung Li ◽  
Diana Dowd
Keyword(s):  
Membrane Vesicles ◽  
Transport Studies ◽  
Isolated Membrane Vesicles

Passive diffusion of nucleosides into Micrococcus sodonensis membrane vesicles

1980 ◽  
Vol 58 (6) ◽  
pp. 457-460 ◽  
Author(s):  
M. A. Pickard
Keyword(s):  
Energy Source ◽  
Membrane Vesicles ◽  
Passive Diffusion ◽  
Membrane Bound ◽  
Nucleoside Uptake ◽  
Membrane Bound Enzymes ◽  
Isolated Membrane Vesicles

Nucleoside entry into isolated membrane vesicles of Micrococcus sodonensis (luteus) was studied using 14C-labelled nucleosides: adenosine, inosine, cytidine, uridine, guanosine, and thymidine. All nucleosides were recovered unmetabolized from the vesicles except adenosine and cytidine which were partly deaminated by membrane-bound enzymes. Vesicle preparations actively transported proline but no energy source was found capable of supporting concentrative nucleoside uptake. The entry of nucleosides into M. sodonensis vesicles was not saturable, nor was there competition between the nucleosides studied for entry. It was concluded that nucleoside entry into M. sodonensis vesicles occurs by passive diffusion.

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