Activation of histone gene transcription in quiescent WI-38 cells or mouse liver by a nonhistone chromosomal protein fraction from HeLa S3 cells

Biochemistry ◽  
1977 ◽  
Vol 16 (16) ◽  
pp. 3713-3721 ◽  
Author(s):  
W. Park ◽  
R. Jansing ◽  
J. Stein ◽  
G. Stein
Keyword(s):  
Gene Transcription ◽  
Protein Fraction ◽  
Mouse Liver ◽  
Histone Gene ◽  
Chromosomal Protein ◽  
Hela S3 ◽  
Histone Gene Transcription ◽  
Hela S3 Cells ◽  
Nonhistone Chromosomal Protein

scholarly journals Human H4 Histone Gene Transcription Requires the Proliferation-Specific Nuclear Factor HiNF-D

1989 ◽  
Vol 264 (25) ◽  
pp. 15034-15042 ◽  
Author(s):  
A J van Wijnen ◽  
K L Wright ◽  
J B Lian ◽  
J L Stein ◽  
G S Stein
Keyword(s):  
Gene Transcription ◽  
Histone Gene ◽  
Nuclear Factor ◽  
Histone Gene Transcription

Repression of histone gene transcription in quiescent 3T6 fibroblasts

1993 ◽  
Vol 217 (2) ◽  
pp. 683-690 ◽  
Author(s):  
Peter ZAHRADKA ◽  
Tracy ELLIOT ◽  
Kenneth HOVLAND ◽  
Dawn E. LARSON ◽  
Laura SAWARD
Keyword(s):  
Gene Transcription ◽  
Histone Gene ◽  
Histone Gene Transcription

scholarly journals Super resolution light microscopy of the Drosophila Histone Locus Body reveals a core-shell organization associated with expression of replication dependent histone genes

2021 ◽  
pp. mbc.E20-10-0645
Author(s):  
James P. Kemp ◽  
Xiao-Cui Yang ◽  
Zbigniew Dominski ◽  
William F. Marzluff ◽  
Robert J. Duronio
Keyword(s):  
Gene Expression ◽  
Gene Transcription ◽  
Nuclear Body ◽  
Histone Gene ◽  
Core Shell ◽  
Histone Genes ◽  
The Core ◽  
Histone Gene Expression ◽  
Histone Locus ◽  
Histone Gene Transcription

The Histone Locus Body (HLB) is an evolutionarily conserved nuclear body that regulates the transcription and processing of replication-dependent (RD) histone mRNAs, which are the only eukaryotic mRNAs lacking a poly-A tail. Many nuclear bodies contain distinct domains, but how internal organization is related to nuclear body function is not fully understood. Here, we demonstrate using structured illumination microscopy that Drosophila HLBs have a “core-shell” organization in which the internal core contains transcriptionally active RD histone genes. The N-terminus of Mxc, which contains a domain required for Mxc oligomerization, HLB assembly, and RD histone gene expression, is enriched in the HLB core. In contrast, the C-terminus of Mxc is enriched in the HLB outer shell as is FLASH, a component of the active U7 snRNP that co-transcriptionally cleaves RD histone pre-mRNA. Consistent with these results, we show biochemically that FLASH binds directly to the Mxc C-terminal region. In the rapid S-M nuclear cycles of syncytial blastoderm Drosophila embryos, the HLB disassembles at mitosis and reassembles the core-shell arrangement as histone gene transcription is activated immediately after mitosis. Thus, the core-shell organization is coupled to zygotic histone gene transcription, revealing a link between HLB internal organization and RD histone gene expression.

Identification of a new set of cell cycle-regulatory genes that regulate S-phase transcription of histone genes in Saccharomyces cerevisiae

1992 ◽  
Vol 12 (11) ◽  
pp. 5249-5259 ◽  
Author(s):  
H Xu ◽  
U J Kim ◽  
T Schuster ◽  
M Grunstein
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Cycle ◽  
Dna Replication ◽  
Gene Transcription ◽  
S Phase ◽  
Histone Gene ◽  
Mrna Synthesis ◽  
Histone Mrna ◽  
Complementation Groups ◽  
Histone Gene Transcription

Histone mRNA synthesis is tightly regulated to S phase of the yeast Saccharomyces cerevisiae cell cycle as a result of transcriptional and posttranscriptional controls. Moreover, histone gene transcription decreases rapidly if DNA replication is inhibited by hydroxyurea or if cells are arrested in G1 by the mating pheromone alpha-factor. To identify the transcriptional controls responsible for cycle-specific histone mRNA synthesis, we have developed a selection for mutations which disrupt this process. Using this approach, we have isolated five mutants (hpc1, hpc2, hpc3, hpc4, and hpc5) in which cell cycle regulation of histone gene transcription is altered. All of these mutations are recessive and belong to separate complementation groups. Of these, only one (hpc1) falls in one of the three complementation groups identified previously by other means (M. A. Osley and D. Lycan, Mol. Cell. Biol. 7:4204-4210, 1987), indicating that at least seven different genes are involved in the cell cycle-specific regulation of histone gene transcription. hpc4 is unique in that derepression occurs only in the presence of hydroxyurea but not alpha-factor, suggesting that at least one of the regulatory factors is specific to histone gene transcription after DNA replication is blocked. One of the hpc mutations (hpc2) suppresses delta insertion mutations in the HIS4 and LYS2 loci. This effect allowed the cloning and sequence analysis of HPC2, which encodes a 67.5-kDa, highly charged basic protein.

scholarly journals Vertebrate histone gene transcription occurs from both DNA strands

1981 ◽  
Vol 9 (7) ◽  
pp. 1591-1598 ◽  
Author(s):  
Sam Bruschi ◽  
Julian R.E. Wells
Keyword(s):  
Gene Transcription ◽  
Histone Gene ◽  
Dna Strands ◽  
Histone Gene Transcription

scholarly journals Stimulation of sea urchin H2B histone gene transcription by a chromatin-associated protein fraction depends on gene sequences downstream of the transcription start site.

1985 ◽  
Vol 5 (10) ◽  
pp. 2764-2769 ◽  
Author(s):  
J Mous ◽  
H Stunnenberg ◽  
O Georgiev ◽  
M L Birnstiel
Keyword(s):  
Sea Urchin ◽  
Protein Fraction ◽  
Transcription Initiation ◽  
Initiation Site ◽  
Histone Gene ◽  
Xenopus Laevis Oocyte ◽  
Chromosomal Protein ◽  
Base Pairs ◽  
Sequence Element ◽  
Associated Proteins

We isolated a chromosomal protein fraction derived from chromatin of sea urchin embryos which specifically stimulated the expression of the histone H2B gene by a factor of 5- to 10-fold when the complete sea urchin histone gene repeat h22 was injected in Xenopus laevis oocyte nuclei. Gene manipulation experiments revealed the existence of two different target sites in the H2B gene which appear to mediate the response to injection of the stimulatory sea urchin chromatin-associated proteins; both are located downstream of the transcription initiation site. The first sequence element which is shown to be implicated is within, or at least includes, the H2B 5' untranslated leader sequence between nucleotides 11 and 76. The second element resides within an H2B DNA segment located near the 3' end of the gene, extending from 90 base pairs upstream of the mRNA 3' terminus to 140 base pairs in the spacer sequences downstream.

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