Transfer RNA in posterior silk gland of Bombyx mori: polyacrylamide gel mapping of mature transfer RNA, identification and partial structural characterization of major isoacceptor species

Biochemistry ◽  
1977 ◽  
Vol 16 (16) ◽  
pp. 3618-3624 ◽  
Author(s):  
Jean Pierre Garel ◽  
Richard L. Garber ◽  
M. A. Q. Siddiqui
Keyword(s):  
Bombyx Mori ◽  
Structural Characterization ◽  
Transfer Rna ◽  
Silk Gland ◽  
Polyacrylamide Gel ◽  
Posterior Silk Gland

The Effect of Formycin on the Processing of Transfer RNA Precursors in the Posterior Silk Gland of Bombyx mori

1977 ◽  
Vol 82 (4) ◽  
pp. 1161-1166 ◽  
Author(s):  
Reiko MAJIMA ◽  
Ken-ichi TSUTSUMI ◽  
Hiroko SUDA ◽  
Kensuke SHIMURA
Keyword(s):  
Bombyx Mori ◽  
Transfer Rna ◽  
Silk Gland ◽  
Posterior Silk Gland

Structural characterization of four ribose-methylated nucleosides from the transfer RNA of extremely thermophilic archaebacteria

1987 ◽  
pp. 909 ◽  
Author(s):  
Charles G. Edmonds ◽  
Pamela F. Crain ◽  
Takeshi Hashizume ◽  
Ramesh Gupta ◽  
Karl O. Stetter ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Transfer Rna

scholarly journals EXTRUSION OF NUCLEAR MATERIALS INTO CYTOPLASM IN THE POSTERIOR SILK GLAND CELLS OF SILKWORM, BOMBYX MORI

1968 ◽  
Vol 36 (3) ◽  
pp. C5-10 ◽  
Author(s):  
Yutaka Tashiro ◽  
Shiro Matsuura ◽  
Takashi Morimoto ◽  
Sunao Nagata
Keyword(s):  
Bombyx Mori ◽  
Silk Gland ◽  
Nuclear Materials ◽  
Gland Cells ◽  
Silkworm Bombyx Mori ◽  
Posterior Silk Gland

scholarly journals STUDIES ON THE POSTERIOR SILK GLAND OF THE SILKWORM, BOMBYX MORI

1968 ◽  
Vol 38 (3) ◽  
pp. 574-588 ◽  
Author(s):  
Yutaka Tashiro ◽  
Takashi Morimoto ◽  
Shiro Matsuura ◽  
Sunao Nagata
Keyword(s):  
Bombyx Mori ◽  
Early Stage ◽  
Larval Instar ◽  
Silk Gland ◽  
Exponential Increase ◽  
Wet Weight ◽  
Rough Er ◽  
Electron Microscopical ◽  
Silkworm Bombyx Mori ◽  
Posterior Silk Gland

Growth of the posterior silk gland and biosynthesis of fibroin during the fifth larval instar of the silkworm, Bombyx mori, have been studied. In accordance with the exponential increase in the wet weight of the gland, the amounts of DNA, RNA, protein, and lipids per animal increased rapidly in the early stage of the fifth instar (0–96 hr). Biosynthesis of fibroin, on the contrary, mainly proceeds in the later stage of the fifth instar (120–192 hr). Electron microscopical observations have shown that, in the very early stage (0–12 hr), a number of free ribosomes exist in the cytoplasm. Rough endoplasmic reticulum (ER) with closely spaced cisternae was also observed. Then rough ER starts to proliferate rapidly, and at the same time lamellar ER is rapidly or gradually transformed into vesicular or tubular forms. In the later stage of the fifth instar (120–192 hr), the cytoplasm is mostly filled with tubular or vesicular ER. Golgi vacuoles, free vacuoles (fibroin globules), and mitochondria are also observed. It is concluded that in the early stage of the fifth instar the cellular structures necessary for the biosynthesis of fibroin are rapidly formed, while in the later stage the biosynthesis of fibroin proceeds at a maximum rate and utilizes these structures.

scholarly journals Isolation of giant silk fibroin polysomes and fibroin mRNP particles using a novel ribonuclease inhibitor, hydroxystilbamidine.

1980 ◽  
Vol 87 (1) ◽  
pp. 292-296 ◽  
Author(s):  
P M Lizardi
Keyword(s):  
Electron Microscopy ◽  
Bombyx Mori ◽  
Silk Fibroin ◽  
Silk Gland ◽  
Biologically Active ◽  
Ribonuclease Inhibitor ◽  
Free System ◽  
Dna And Rna ◽  
Free Translation ◽  
Posterior Silk Gland

Hydroxystilbamidine isethionate, a dye capable of binding to both DNA and RNA, has been found to be a powerful inhibitor of cellular ribonucleases. A procedure has been developed that, with the aid of this compound, permits the preparative isolation of giant silk fibroin polyribosomes from the posterior silk gland of Bombyx mori. The polyribosomes contain approximately 45-112 ribosomal particles, as judged by electron microscopy. Treatment of giant fibroin polyribosomes with EDTA releases a particle that sediments at 125S. This mRNP particle contains biologically active silk fibroin mRNA, as judged by cell-free translation in an mRNA-dependent reticulocyte cell-free system.

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