Isolation and characterization of bovine factor XII (Hageman factor)

Biochemistry ◽  
1977 ◽  
Vol 16 (10) ◽  
pp. 2270-2278 ◽  
Author(s):  
Kazuo Fujikawa ◽  
Kenneth A. Walsh ◽  
Earl W. Davie
Keyword(s):  
Factor Xii ◽  
Hageman Factor ◽  
Isolation And Characterization ◽  
Bovine Factor

scholarly journals THE FIRST COMPONENT OF THE KININ-FORMING SYSTEM IN HUMAN AND RABBIT PLASMA

1971 ◽  
Vol 134 (4) ◽  
pp. 986-1004 ◽  
Author(s):  
C. G. Cochrane ◽  
K. D. Wuepper
Keyword(s):  
Diatomaceous Earth ◽  
Chelating Agents ◽  
Gel Filtration ◽  
Clotting Factor ◽  
Factor Xii ◽  
Rabbit Plasma ◽  
Hageman Factor ◽  
Isolation And Characterization ◽  
Hexadimethrine Bromide

The isolation and characterization of the first component of the kinin-forming system in human and rabbit plasma are presented. Functionally, the molecule is the precursor of the activator of prekallikrein (Pre-PKA) and evidence is presented that it is identical with Hageman factor (clotting factor XII). The component from each plasma possessed similar characteristics. This molecule was found to have a mol wt of 110,000 and sedimentation rate of 4.6S. It migrated in electrophoresis as a ß-globulin, having an isoelectric point of 6.1. Upon activation with glass, kaolin, diatomaceous earth, ellagic acid, or trypsin, the activated molecule converted purified prekallikrein (prokininogenase) to the active enzyme. Clot-promoting activity was associated with the capacity to activate prekallikrein through each procedure of isolation. The clot-promoting factor was in precursor form, requiring treatment with kaolin or trypsin to gain activity. Evidence indicated that the protein was Hageman factor (factor XII): it promoted clotting of factor XII-deficient, but not Factor XI- or IX-deficient plasma, and did not convert fibrinogen to fibrin it bound to and was activated by kaolin or other negatively charged particles in the presence of chelating agents; the activation by kaolin could be prevented by pretreating the kaolin with hexadimethrine bromide (H Br); prekallikrein-activating and clot-promoting activities were identical in their physical properties; and the prekallikrein activator could not be detected in Hageman factor-deficient plasma. Activation of Hageman factor was accompanied by cleavage of the molecule into several fragments, one of which possessed prekallikrein-activating (PKA) and clot-promoting properties. The PKA fragment sedimented at 2.6S and by gel filtration was found to have a molecular weight of 32,000. The PKA possessed only 1/50 the clot-promoting capacity of the freshly activated native molecule.

scholarly journals Production and characterization of a murine monoclonal antibody against a heavy chain of Hageman factor (factor XII)

Blood ◽  
1985 ◽  
Vol 65 (5) ◽  
pp. 1263-1268 ◽  
Author(s):  
H Saito ◽  
T Ishihara ◽  
H Suzuki ◽  
T Watanabe
Keyword(s):  
Monoclonal Antibody ◽  
Heavy Chain ◽  
Procoagulant Activity ◽  
Factor Xii ◽  
Contact Activation ◽  
Hageman Factor ◽  
Heavy Chains ◽  
Charged Surfaces ◽  
Normal Human

Abstract A murine hybridoma cell line that produces a monoclonal antibody to human Hageman factor (HF, factor XII) is described. The antibody (P 5–2– 1) consists of mouse IgG2b heavy chains and lambda light chains, selectively neutralizes HF procoagulant activity, and prevents the proteolytic cleavage of HF during contact activation in plasma. When HF is exposed to P 5–2–1 before the absorption of HF to kaolin, HF procoagulant activity is markedly inhibited. In contrast, P 5–2–1 does not interfere with HF activity after the adsorption of HF to kaolin. P 5–2–1 does not inactivate the prekallikrein–activating activity of 28,000–mol wt HF fragments (HFf). P 5–2–1 binds exclusively to the 40,000mol wt portion of a heavy chain of HF and inhibits the adsorption of HF to negatively charged surfaces. P 5–2–1 immobilized on Sepharose can be used to deplete HF from normal human plasma. This immunoaffinity-depleted plasma is indistinguishable from congenital HF- deficient plasma and can be used as the substrate for HF procoagulant activity assay.

Isolation and characterization of bovine factor IX (Christmas factor)

Biochemistry ◽  
1973 ◽  
Vol 12 (24) ◽  
pp. 4938-4945 ◽  
Author(s):  
Kazuo Fujikawa ◽  
Arthur R. Thompson ◽  
Mark E. Legaz ◽  
Robert G. Meyer ◽  
Earl W. Davie
Keyword(s):  
Factor Ix ◽  
Isolation And Characterization ◽  
Bovine Factor

Isolation and characterization of bovine factor XI (plasma thromboplastin antecedent)

Biochemistry ◽  
1977 ◽  
Vol 16 (10) ◽  
pp. 2279-2286 ◽  
Author(s):  
Takehiko Koide ◽  
Hisao Kato ◽  
Earl W. Davie
Keyword(s):  
Factor Xi ◽  
Isolation And Characterization ◽  
Bovine Factor

Isolation and characterization of bovine factor VII

Biochemistry ◽  
1975 ◽  
Vol 14 (22) ◽  
pp. 4928-4934 ◽  
Author(s):  
Walter Kisiel ◽  
Earl W. Davie
Keyword(s):  
Factor Vii ◽  
Isolation And Characterization ◽  
Bovine Factor

Activation of bovine factor XII (Hageman factor) by plasma kallikrein

Biochemistry ◽  
1980 ◽  
Vol 19 (7) ◽  
pp. 1322-1330 ◽  
Author(s):  
Kazuo Fujikawa ◽  
Ronald L. Heimark ◽  
Kotoku Kurachi ◽  
Earl W. Davie
Keyword(s):  
Plasma Kallikrein ◽  
Factor Xii ◽  
Hageman Factor ◽  
Bovine Factor

scholarly journals The Isolation and Characterization of Bovine Factor VIII (Antihemophilic Factor)

1972 ◽  
Vol 247 (8) ◽  
pp. 2512-2521
Author(s):  
Gottfried Schmer ◽  
Edward P. Kirby ◽  
David C. Teller ◽  
Earl W. Davie
Keyword(s):  
Factor Viii ◽  
Isolation And Characterization ◽  
Antihemophilic Factor ◽  
Bovine Factor

Characterization of bovine factor XIIa (activated Hageman factor)

Biochemistry ◽  
1977 ◽  
Vol 16 (19) ◽  
pp. 4182-4188 ◽  
Author(s):  
Kazuo Fujikawa ◽  
Kotoku Kurachi ◽  
Earl W. Davie
Keyword(s):  
Hageman Factor ◽  
Factor Xiia ◽  
Bovine Factor

scholarly journals Isolation and characterization of single chain bovine factor V.

1979 ◽  
Vol 254 (2) ◽  
pp. 508-517 ◽  
Author(s):  
M.E. Nesheim ◽  
K.H. Myrmel ◽  
L. Hibbard ◽  
K.G. Mann
Keyword(s):  
Factor V ◽  
Single Chain ◽  
Isolation And Characterization ◽  
Bovine Factor
Sign in / Sign up

Export Citation Format

Share Document