Allergic encephalomyelitis: evidence for lack of significant encephalitogenic activity of purified peptide L in the monkey

Biochemistry ◽  
1978 ◽  
Vol 17 (21) ◽  
pp. 4571-4577 ◽  
Author(s):  
Yashwant D. Karkhanis ◽  
Johanna Y. Zeltner ◽  
Richard L. Anderson ◽  
Dennis J. Carlo
Keyword(s):  
Allergic Encephalomyelitis ◽  
Encephalitogenic Activity

scholarly journals Commercial horseradish peroxidase degrades myelin encephalitogenic protein during coupling for immunohistochemical studies.

1977 ◽  
Vol 25 (5) ◽  
pp. 329-336 ◽  
Author(s):  
A B Johnson ◽  
W Cammer
Keyword(s):  
Horseradish Peroxidase ◽  
Experimental Allergic Encephalomyelitis ◽  
Gel Electrophoresis ◽  
Basic Protein ◽  
Biological Activities ◽  
Allergic Encephalomyelitis ◽  
Rapid Loss ◽  
Immunohistochemical Studies ◽  
Encephalitogenic Activity ◽  
Experimental Allergic

Conjugates of myelin encephalitogenic basic protein (EP) and commercial horseradish peroxidase (HRP) have been used for immunohistochemical demonstrations of anti-EP antibody in animals with experimental allergic encephalomyelitis. We performed gel electrophoresis studies on EP-HRP conjugates prepared with glutaraldehyde and on mixtures of EP and HRP incubated without glutaraldehyde. The results show that under conditions of one-and two-step coupling HRP causes rapid loss of the native EP band, apparently due to EP degradation. The EP-HRP mixtures are not encephalitogenic in rabbits, or encephalitogenic activity is lost during processing. The immunohistochemical reactivity of conjugates, however, signals some preservation of antibody-combining sites. The mechanism of the HRP effect on EP is unknown. The possibilities of a contaminating proteinase or direct peroxidatic attack are suggested. Until this action of HRP can be overcome, the effect of coupling procedures on the biological activities of EP will be difficult to assess, and EP-HRP conjugates cannot be expected to reveal sites that may bind encephalitogenic portions of the EP molecule.

scholarly journals Allergic Encephalomyelitis

1971 ◽  
Vol 246 (10) ◽  
pp. 3418-3424
Author(s):  
E.H. Eylar ◽  
Fred C. Westall ◽  
Steven Brostoff
Keyword(s):  
Allergic Encephalomyelitis
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