Active site directed irreversible inhibition of thermolysin

David Rasnick; James C. Powers

doi:10.1021/bi00614a002

Active-site tyrosyl residues are targets in the irreversible inhibition of a class Mu glutathione transferase by 2-(S-glutathionyl)-3,5,6-trichloro-1,4-benzoquinone.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)47103-7 ◽

1994 ◽

Vol 269 (43) ◽

pp. 26890-26897

Author(s):

J H Ploemen ◽

W W Johnson ◽

S Jespersen ◽

D Vanderwall ◽

B van Ommen ◽

...

Keyword(s):

Active Site ◽

Glutathione Transferase ◽

Irreversible Inhibition

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Irreversible inhibition of bovine lung angiotensin I-converting enzyme with p-[N,N-bis(chloroethyl)amino]phenylbutyric acid (chlorambucil) and chlorambucyl L-proline and with evidence that an active site carboxyl group is labeled.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)68269-4 ◽

1982 ◽

Vol 257 (2) ◽

pp. 811-815

Author(s):

R.B. Harris ◽

I.B. Wilson

Keyword(s):

Active Site ◽

Carboxyl Group ◽

Converting Enzyme ◽

Angiotensin I ◽

Irreversible Inhibition ◽

Angiotensin I Converting Enzyme ◽

Phenylbutyric Acid

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Exploring the Active Site ofTrypanosoma bruceiPhosphofructokinase by Inhibition Studies: Specific Irreversible Inhibition†

Biochemistry ◽

10.1021/bi020082z ◽

2002 ◽

Vol 41 (32) ◽

pp. 10183-10193 ◽

Cited By ~ 16

Author(s):

Samantha Claustre ◽

Colette Denier ◽

Faouzi Lakhdar-Ghazal ◽

Andrée Lougare ◽

Claudia Lopez ◽

...

Keyword(s):

Active Site ◽

Irreversible Inhibition ◽

Inhibition Studies

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ChemInform Abstract: Isopentenyldiphosphate Isomerase. Mechanism of Active-Site-Directed Irreversible Inhibition by 3-(Fluoromethyl)-3-butenyl Diphosphate

ChemInform ◽

10.1002/chin.198934071 ◽

1989 ◽

Vol 20 (34) ◽

Author(s):

C. D. POULTER ◽

M. MUEHLBACHER ◽

D. R. DAVIS

Keyword(s):

Active Site ◽

Irreversible Inhibition

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Active site-directed irreversible inhibition of glutathione S-transferases by the glutathione conjugate of tetrachloro-1,4-benzoquinone.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)37652-x ◽

1988 ◽

Vol 263 (26) ◽

pp. 12939-12942 ◽

Cited By ~ 6

Author(s):

B van Ommen ◽

C den Besten ◽

A L Rutten ◽

J H Ploemen ◽

R M Vos ◽

...

Keyword(s):

Active Site ◽

Glutathione Conjugate ◽

Irreversible Inhibition ◽

Glutathione S Transferases

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Active-site directed irreversible inhibition of diamine oxidase by a homologous series of aziridinylalkylamines

Biochemical Pharmacology ◽

10.1016/0006-2952(92)90392-v ◽

1992 ◽

Vol 44 (6) ◽

pp. 1229-1232 ◽

Cited By ~ 4

Author(s):

Joe W. Conner ◽

Yuan Zhi-Min ◽

Patrick S. Callery

Keyword(s):

Active Site ◽

Homologous Series ◽

Diamine Oxidase ◽

Irreversible Inhibition

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Active-site-directed irreversible inhibition of glycosidases by the corresponding glycosylmethyl-(p-nitrophenyl)triazenes

Journal of the Chemical Society Perkin Transactions 1 ◽

10.1039/p19810000366 ◽

1981 ◽

pp. 366 ◽

Cited By ~ 23

Author(s):

Philip J. Marshall ◽

Michael L. Sinnott ◽

Paul J. Smith ◽

Danny Widdows

Keyword(s):

Active Site ◽

Irreversible Inhibition

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Active-site-directed irreversible inhibition of 4-aminobutyrate aminotransferase by ethanolamine O-sulphate

Biochemical Journal ◽

10.1042/bj1300080pb ◽

1972 ◽

Vol 130 (2) ◽

pp. 80P-81P ◽

Cited By ~ 1

Author(s):

L J Fowler ◽

R A John

Keyword(s):

Active Site ◽

Aminobutyrate Aminotransferase ◽

Irreversible Inhibition

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Detection of a transient enzyme-steroid complex during active-site-directed irreversible inhibition of 3-oxo-.DELTA.5-steroid isomerase

Biochemistry ◽

10.1021/bi00366a026 ◽

1986 ◽

Vol 25 (18) ◽

pp. 5159-5164 ◽

Cited By ~ 12

Author(s):

Charles L. Bevins ◽

Ralph M. Pollack ◽

Robert H. Kayser ◽

Patricia L. Bounds

Keyword(s):

Active Site ◽

Irreversible Inhibition

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The Effect of P-Chlorophenylalanine on the Action of Potassium Ions on Mytilus Cilia

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010011708x ◽

1975 ◽

Vol 33 ◽

pp. 692-693

Author(s):

Anthony A. Paparo ◽

Judith A. Murphy

Keyword(s):

Paper Chromatography ◽

Active Site ◽

Tryptophan Hydroxylase ◽

Ciliary Activity ◽

Potassium Ions ◽

Nervous Control ◽

Irreversible Inhibition ◽

Rate Limiting

The cilioexcitatory effects of 5-hydroxytryptamine(5-HT) has been observed in Mytilus. Its presence has been demonstrated by photofluorometry, bioassay and paper chromatography. Enzymes have been proved to exist not only for the biosynthesis of 5-HT, but also for its oxidation. The evidence thus far indicates that 5-HT is the principal endogenous cilioexcitatory substance involved in the nervous control of ciliary activity in the gill of Mytilus. The available evidence suggests that the decrease in 5-HT after administration of p-chlorophenylalanine(PCPA) is mediated by a decrease in the synthesis of 5-HT resulting from an inhibition of the rate limiting enzyme, tryptophan hydroxylase. Investigators propose that PCPA is incorporated into tryptophan hydroxylase at or near the active site and is responsible for the irreversible inhibition which persists for several days. The present study investigates the effect of PCPA on potassium(K) enhancement of ciliary activity.

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