Photochemical cross-linking studies on the interaction of Escherichia coli RNA polymerase with T7 DNA

Biochemistry ◽  
1978 ◽  
Vol 17 (15) ◽  
pp. 2954-2961 ◽  
Author(s):  
Zaharia Hillel ◽  
Cheng-Wen Wu
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Cross Linking

scholarly journals DksA regulates RNA polymerase in Escherichia coli through a network of interactions in the secondary channel that includes Sequence Insertion 1

2015 ◽  
Vol 112 (50) ◽  
pp. E6862-E6871 ◽  
Author(s):  
Andrey Parshin ◽  
Anthony L. Shiver ◽  
Jookyung Lee ◽  
Maria Ozerova ◽  
Dina Schneidman-Duhovny ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Active Site ◽  
Amino Acid Starvation ◽  
Cross Linking ◽  
Molecular Function ◽  
Secondary Channel ◽  
Global Response ◽  
Microbial Life ◽  
Active Site Region

Sensing and responding to nutritional status is a major challenge for microbial life. In Escherichia coli, the global response to amino acid starvation is orchestrated by guanosine-3′,5′-bisdiphosphate and the transcription factor DksA. DksA alters transcription by binding to RNA polymerase and allosterically modulating its activity. Using genetic analysis, photo–cross-linking, and structural modeling, we show that DksA binds and acts upon RNA polymerase through prominent features of both the nucleotide-access secondary channel and the active-site region. This work is, to our knowledge, the first demonstration of a molecular function for Sequence Insertion 1 in the β subunit of RNA polymerase and significantly advances our understanding of how DksA binds to RNA polymerase and alters transcription.

scholarly journals Stringent control in Escherichia coli applies also to transcription by T7 RNA polymerase.

1987 ◽  
Vol 262 (9) ◽  
pp. 3940-3943
Author(s):  
M. Yamagishi ◽  
J.R. Cole ◽  
M. Nomura ◽  
F.W. Studier ◽  
J.J. Dunn
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
T7 Rna Polymerase ◽  
Stringent Control
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