Covalent structure of a group-specific protease from rat small intestine. Appendix: Crystallographic data for a group specific protease from rat intestine

Biochemistry ◽  
1978 ◽  
Vol 17 (5) ◽  
pp. 811-819 ◽  
Author(s):  
Richard G. Woodbury ◽  
Nobuhiko Katunuma ◽  
Keiko Kobayashi ◽  
Koiti Titani ◽  
Hans Neurath ◽  
...  
Keyword(s):  
Small Intestine ◽  
Crystallographic Data ◽  
Rat Small Intestine ◽  
Rat Intestine ◽  
Covalent Structure ◽  
Specific Protease

Apolipoprotein A-IV synthesis in rat intestine: regulation by dietary triglyceride

1987 ◽  
Vol 252 (5) ◽  
pp. G662-G666 ◽  
Author(s):  
T. F. Apfelbaum ◽  
N. O. Davidson ◽  
R. M. Glickman
Keyword(s):  
Protein Synthesis ◽  
Small Intestine ◽  
Total Protein ◽  
Mrna Levels ◽  
Rat Small Intestine ◽  
Rat Intestine ◽  
Apolipoprotein A ◽  
Rat Enterocytes

Apolipoprotein A-IV (apoA-IV) synthesis rates were measured in vivo in rat enterocytes by immunoprecipitation after administration of [3H]leucine into in situ loops of jejunum and ileum. Basal apoA-IV synthesis rates (percent total protein synthesis) were significantly higher in jejunal enterocytes (2.05 +/- 0.54%) compared with ileal enterocytes (0.48 +/- 0.32%) from the same fasted animals. After an acute triglyceride bolus, significant and sustained elevations of apoA-IV synthesis rates were seen in both jejunal and ileal enterocytes with maximal effects noted at 4-6 h. Animals fed diets containing 30% wt/wt triglyceride as saturated (SF) or polyunsaturated (UF) fats for 6 wk had similarly increased rates of apoA-IV synthesis in jejunal enterocytes with both SF (3.73 +/- 0.83%) and UF (3.33 +/- 0.64%) but no change in ileal enterocytes. By contrast, animals consuming a fat-free diet for 3 wk had jejunal apoA-IV synthesis rates indistinguishable from basal values (2.40 +/- 0.45%). Translatable intestinal mRNA levels for pre-apoA-IV after triglyceride increased in parallel to synthesis rates with a 50% increase in jejunum and a 350% increase in ileum observed at 4-6 h. These results suggest that apoA-IV synthesis by rat small intestine increases in response to acute and chronic dietary triglyceride, is maintained in the absence of dietary triglyceride, and may be under pretranslational control.

scholarly journals Tryptophan 5-hydroxylase in rat intestine

1973 ◽  
Vol 131 (2) ◽  
pp. 375-380 ◽  
Author(s):  
T. Noguchi ◽  
M. Nishino ◽  
R. Kido
Keyword(s):  
Enzyme Activity ◽  
Small Intestine ◽  
Physiological Significance ◽  
Rat Small Intestine ◽  
Rat Intestine ◽  
Ph Optimum ◽  
Full Activity

Tryptophan 5-hydroxylase was partially purified from rat small intestine and characterized. The enzyme activity was mainly localized in the distal one-fourth of the small intestine. The enzyme required Fe2+, 2-amino-4-hydroxy-6,7-dimethyl-5,6,7,8-tetrahydropteridine and oxygen for full activity. The pH optimum of the reaction was 8.0. The hydroxylation rate of d-tryptophan by the enzyme was one-third that of l-tryptophan. l-Phenylalanine and l-tyrosine could not serve as substrates. The physiological significance of the enzyme is discussed.

POSSIBILITY OF ORALLY ADMINISTERED L-LYSINE-A-OXIDASE INTERNALIZATION IN THE MODEL OF ISOLATED CUT OF RAT SMALL INTESTINE

2019 ◽  
Vol 65 (3) ◽  
pp. 463-466
Author(s):  
Gulalek Babaeva ◽  
Yelena Lukasheva ◽  
Zhanneta Cherkasova ◽  
Yelena Treshchalina ◽  
Natalya Andronova ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Small Intestine ◽  
Incubation Medium ◽  
Rat Small Intestine ◽  
Administration Route ◽  
Rat Intestine ◽  
Antitumor Effects ◽  
Luminescence Method ◽  
Parenteral Dose ◽  
Oral Therapeutic

Enzyme L-lysine а-oxidase (LO) exhibits significant antitumor effects by parenteral administration and is promising for clinical trials, particularly in the case of colorectal cancer. The fungi Trichoderma cf.aureoviride Rifai VKM F-4268D is a source of LO. Since there is evidence in the literature of oral use of proteins for therapeutic purposes, it seemed promising to investigate the possibility of such administration route for LO. The goal of the work was to determine the ability of LO to be internalized by the rat small intestine. LO was labeled by Ac-ridinium (LO-Acridinium). Experiments were performed on the rat model using isolated inverted segments of small intestine. The inverted segments were immersed into incubation medium, containing LO-Acridinium. After 30 minutes the samples were taken from the incubation medium and from the intestine segments and relative luminescence was determined by standard flash luminescence method. The amount of absorbed LO-Ac-ridinium was estimated to be 11% for the entire length of the small rat intestine. Based on the optimal total parenteral dose of 400 U/kg for mice the total dose when administered orally was estimated as 4000 U/kg. The absorption of LO through the wall of the rat small intestine was quantitatively characterized, the possibility of its oral administration was proved, and the oral therapeutic dose for mice was estimated.

scholarly journals Aromatic amino acid transaminase in rat intestine

1973 ◽  
Vol 135 (4) ◽  
pp. 815-818 ◽  
Author(s):  
Jun Nakamura ◽  
Tomoo Noguchi ◽  
Ryo Kido
Keyword(s):  
Amino Acids ◽  
Small Intestine ◽  
Amino Acid ◽  
Aromatic Amino Acid ◽  
Partial Purification ◽  
Rat Small Intestine ◽  
Purification And Characterization ◽  
Rat Intestine ◽  
Enzyme Preparations

The transamination of aromatic l-amino acids (5-hydroxytryptophan, tryptophan, tyrosine, phenylalanine and kynurenine) was shown to be catalysed by enzyme preparations from rat small intestine. On the basis of the partial purification and characterization of these aromatic amino acid transaminases, it is suggested that rat small intestine contains several kinds of aromatic amino acid transaminases.

scholarly journals Effects of short-term insulin deficiency on lipogenesis and cholesterol synthesis in rat small intestine and liver in vivo

1985 ◽  
Vol 231 (1) ◽  
pp. 221-223 ◽  
Author(s):  
D H Williamson ◽  
V Ilic ◽  
J Hughes
Keyword(s):  
Small Intestine ◽  
Cholesterol Synthesis ◽  
Physiological Significance ◽  
Oral Glucose ◽  
Rat Small Intestine ◽  
Insulin Deficiency ◽  
Rat Intestine ◽  
Short Term ◽  
Glucose Loading

The rate of lipogenesis in rat intestine increased on oral glucose loading and decreased after induction of acute insulin deficiency with streptozotocin. The latter effects could be partially reversed by administration of insulin. Parallel changes in the rate of lipogenesis were found in liver. In contrast, insulin deficiency did not alter the rate of cholesterol synthesis in intestine, but decreased it in liver. The physiological significance of the regulation of intestinal lipogenesis by insulin is discussed.

Experiments on the reproduction ofNippostrongylus brasiliensisin the rat intestine

Parasitology ◽  
1970 ◽  
Vol 61 (2) ◽  
pp. 317-322 ◽  
Author(s):  
R. F. Phillipson
Keyword(s):  
Small Intestine ◽  
Egg Production ◽  
Seminal Receptacle ◽  
Rat Small Intestine ◽  
Rat Intestine ◽  
Adult Females ◽  
Minimum Requirements ◽  
Ventral Wall

A method is described for producing unisexual infections ofN. brasiliensisin rats; this method was used primarily to obtain populations of virgin adult females. Experiments were carried out in which male worms were introduced to virgin females in the rat small intestine. The worms were recovered after periods varying from 30 min to 22 h and the females were examined for fertile egg production or other evidence of insemination.In some experiments a few females were inseminated within 30 min of being mixed with males, but for reliable results a period of over 1 h was necessary. Sperm were first deposited along the ventral wall of the vagina and there was evidence that a single insemination could fill the vagina. After 4·5 h the majority of females had been inseminated and this period was long enough for sperm to reach the seminal receptacle in one female. A minority of females had a few fertile eggs in the uterus by 5·5 h, and fertile eggs were being passed by 6·0 h after the introduction of males.Small numbers of males were introduced to an excess of virgin females in order to obtain information on the sexual capacity of males. These experiments indicated that males could, on average, fertilize one female every 2 h during the first 15–20 h. This potential was shown to be greatly in excess of the minimum requirements for maintaining the maximum fertile egg output of females in normal infections.

In situ demonstration of migration of dendritic cells released from rat small intestine to mesenteric lymph nodes

2001 ◽  
Vol 120 (5) ◽  
pp. A183-A183
Author(s):  
H KOBAYASHI ◽  
H NAGATA ◽  
S MIURA ◽  
T AZUMA ◽  
H SUZUKI ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Small Intestine ◽  
Lymph Nodes ◽  
Rat Small Intestine ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph

Anti-inflammatory effect of STW 5, STW 6 and isolated extracts on the rat small intestine

2008 ◽  
Vol 29 (S 1) ◽  
Author(s):  
K Nieber ◽  
S Michael ◽  
K Grötzinger ◽  
JW Rauwald ◽  
O Kelber
Keyword(s):  
Small Intestine ◽  
Rat Small Intestine ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Oral vanadate reduces Na(+)-dependent glucose transport in rat small intestine

Diabetes ◽  
1993 ◽  
Vol 42 (8) ◽  
pp. 1126-1132 ◽  
Author(s):  
K. L. Madsen ◽  
V. M. Porter ◽  
R. N. Fedorak
Keyword(s):  
Small Intestine ◽  
Glucose Transport ◽  
Rat Small Intestine
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