Circular dichroism study of the solution conformation of luteinizing hormone releasing hormone

Biochemistry ◽  
1979 ◽  
Vol 18 (26) ◽  
pp. 5776-5781 ◽  
Author(s):  
John R. Cann ◽  
K. Channabasavaiah ◽  
John M. Stewart
Keyword(s):  
Circular Dichroism ◽  
Luteinizing Hormone ◽  
Solution Conformation ◽  
Luteinizing Hormone Releasing Hormone ◽  
Releasing Hormone ◽  
Circular Dichroïsm

The solution conformation of the luteinizing hormone-releasing hormone (LHRH) anti-sense and reversed anti-sense peptides

1990 ◽  
Vol 68 (7) ◽  
pp. 1083-1091
Author(s):  
Albin Otter ◽  
Paul G. Scott ◽  
John M. Stewart ◽  
George Kotovych
Keyword(s):  
Amino Acids ◽  
Luteinizing Hormone ◽  
Two Dimensions ◽  
Helical Conformation ◽  
Solution Conformation ◽  
Luteinizing Hormone Releasing Hormone ◽  
Releasing Hormone ◽  
Fast Exchange ◽  
Helical Turn ◽  
C Nmr

The LHRH anti-sense peptide (Ser1-Arg2-Ala3-Gln4-Ser5-Ile6-Gly7-Pro8-Val9-Leu10) and the inversed sequence, the LHRH inversed anti-sense peptide, have been studied by 1H and 13C NMR in one and two dimensions. The spectroscopic evidence, obtained in CD3OH/H2O (60/40) solution at pH 3.6, indicates that the first four amino acids of the anti-sense peptide form one complete 310-helical turn followed by an extended conformation for the rest of the peptide. On the time-averaged NMR scale the molecules in the 310-helical conformation are in fast exchange with the molecules that are extended throughout (major conformer). The reversed anti-sense peptide exhibits less discernible features. It appears likely that a small percentage of the molecules form a β-turn in the C-terminal four residues. Another β-turn might be located in the Val2-Ile5 fragment. Both turns occur in very minor concentrations only, and therefore it is impossible to define what kind of β-turns are present. Keywords: LHRH anti-sense, inversed anti-sense, conformation, NMR.

EFFECT OF CORTISOL OR ADRENOCORTICOTROPHIN ON RELEASE OF LUTEINIZING HORMONE INDUCED BY LUTEINIZING HORMONE RELEASING HORMONE IN THE DAIRY HEIFER

1982 ◽  
Vol 92 (1) ◽  
pp. 141-146 ◽  
Author(s):  
R. L. MATTERI ◽  
G. P. MOBERG
Keyword(s):  
Luteinizing Hormone ◽  
Luteinizing Hormone Releasing Hormone ◽  
Releasing Hormone ◽  
Dairy Heifers ◽  
Significant Difference ◽  
Significant Depression ◽  
Lh Rh ◽  
Lh Releasing Hormone ◽  
And Control ◽  
Slight Depression

During treatment with cortisol or ACTH, dairy heifers were given two doses of LH releasing hormone (LH-RH) spaced 1·5 h apart. Serum concentrations of cortisol and LH were monitored during each treatment. Treatment with both ACTH and cortisol raised plasma cortisol levels above the respective saline controls (P<0·001). Neither treatment affected basal LH concentrations. A slight depression in LH response was seen in the cortisol-treated animals after the first LH-RH injection, as shown by a statistically significant depression at three of the sample times. There was no significant difference between treated and control LH values after the second LH-RH administration. Treatment with ACTH resulted in significantly reduced LH values at all sample times after both injections of LH-RH.

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