Amino acid sequence studies on the .alpha. chain of human fibrinogen. Overlapping sequences providing the complete sequence

Biochemistry ◽  
1979 ◽  
Vol 18 (24) ◽  
pp. 5410-5416 ◽  
Author(s):  
K. W. K. Watt ◽  
B. A. Cottrell ◽  
D. D. Strong ◽  
R. F. Doolittle
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Complete Sequence ◽  
Human Fibrinogen ◽  
Alpha Chain

scholarly journals On the Primary Structure of Human Fibrinogen

1977 ◽  
Author(s):  
A. Henschen ◽  
F. Lottspeich ◽  
E. Töpfer-Petersen ◽  
R. Warbinek
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Primary Structure ◽  
Cyanogen Bromide ◽  
Attachment Site ◽  
Complete Sequence ◽  
Cleavage Sites ◽  
Amino Acid Residues ◽  
Human Fibrinogen ◽  
Β Chain

The aim of the present investigation is to elucidate the primary structure of human fibrinogen. Through the work of several laboratories including our own large parts of the structure are now known. Here will be reported the complete amino acid sequence of the γ-chain (409 residues). Furthermore, the carbohydrate linkage site in the β-chain and plasmin cleavage sites in the β- and γ-chains have been identified.The peptide chains were isolated by CM-cellulose chromatography following mercaptolysis and alkylation. The γ-chain was cleaved in a way to produce large fragments suitable for automatic sequencing, e. g. with cyanogen bromide or trypsin after citraconylation. The sequences of the isolated fragments allowed reconstruction of the complete sequence of the γ-chain.The carbohydrate linkage site in the β-chain could be isolated by affinity chromatography on concanavalin A-agarose following cleavage of the chain by trypsin or cyanogen bromide. The sequence of 21 amino acid residues around the carbohydrate attachment site was determined.The plasmin cleavage site giving rise to N-terminal glycine in the γ-chain D-fragment was identified. The amino acid sequence linking plasmic fragments E and D in the β-chain was determined.

Amino acid sequence studies on the .alpha. chain of human fibrinogen. Exact location of crosslinking acceptor sites

Biochemistry ◽  
1979 ◽  
Vol 18 (24) ◽  
pp. 5405-5410 ◽  
Author(s):  
B. A. Cottrell ◽  
D. D. Strong ◽  
K. W. K. Watt ◽  
R. F. Doolittle
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Human Fibrinogen ◽  
Alpha Chain ◽  
Exact Location

Hemoglobin from the Antarctic fish Notothenia coriiceps neglecta. 2. Amino acid sequence of the alpha chain of Hb 1

1989 ◽  
Vol 179 (3) ◽  
pp. 707-713 ◽  
Author(s):  
Rossana D'AVINO ◽  
Carla CARUSO ◽  
Mario ROMANO ◽  
Laura CAMARDELLA ◽  
Bruno RUTIGLIANO ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Antarctic Fish ◽  
Alpha Chain ◽  
Notothenia Coriiceps ◽  
The Antarctic
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