Effects of anions on the activation thermodynamics and fluorescence emission spectrum of alkaline phosphatase: evidence for enzyme hydration changes during catalysis

Biochemistry ◽  
1979 ◽  
Vol 18 (24) ◽  
pp. 5322-5332 ◽  
Author(s):  
George S. Greaney ◽  
George N. Somero
Keyword(s):  
Alkaline Phosphatase ◽  
Emission Spectrum ◽  
Fluorescence Emission ◽  
Fluorescence Emission Spectrum

scholarly journals The Synthesis and Application of Nitrogen-Doped Graphene Quantum Dots on Brilliant Blue Detection

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Li Jin ◽  
Ying Wang ◽  
Fengkai Yan ◽  
Jianpo Zhang ◽  
Fangli Zhong
Keyword(s):  
Quantum Dots ◽  
Emission Spectrum ◽  
Graphene Quantum Dots ◽  
Fluorescence Emission ◽  
Fluorescence Decay ◽  
Brilliant Blue ◽  
Fluorescence Emission Spectrum ◽  
Nitrogen Doped ◽  
Nitrogen Doped Graphene ◽  
Doped Graphene

Nitrogen-doped graphene quantum dots had been successfully synthesized and characterized by using transmission electron microscope, X-ray photoelectron spectroscopy, absorbance spectrum, fluorescence emission spectrum, and fluorescence decay curve. TEM results indicated that the diameters of the as-prepared nitrogen-doped graphene quantum dots were in the range of 2 - 5 nm and the lattice space is about 0.276 nm; Raman spectrum result indicated that there were two characteristic peaks, generally named D (~1408 cm−1) and G (~1640 cm−1) bands; both TEM and Raman spectrum results indicated that the as-synthesized product was graphene quantum dots. Deconvoluted high resolution XPS spectra for C1s, O1s, and N1s results indicated that there are -NH-, -COOH, and -OH groups on the surface of nitrogen-doped graphene quantum dot. Fluorescence emission spectrum indicated that the maximum fluorescence emission spectrum of nitrogen-doped graphene quantum dots was blue shift about 30.1 nm and the average fluorescence decay time of nitrogen-doped graphene quantum dots increased about 2 ns, compared with graphene quantum dots without doping of nitrogen. Then, the as-prepared nitrogen-doped graphene quantum dots were used to quantitatively analyze brilliant blue based on the fluorescent quenching of graphene quantum dots, and the effect of pH and reaction time on this fluorescent quenching system was also obtained. Under selected condition, the linear regression equations were F0/F=0.0087 (brilliant blue) + 0.9553 and F0/F=0.01205 (brilliant blue) + 0.6695, and low detection limit was 3.776 μmol/L (3.776 nmol/mL). Once more diluted N-GQDs (0.05 mg/mL) were used, the low detection limit could reach 94.87 nmol/L. Then, temperature-dependent experiment, absorbance spectra, and dynamic fluorescence quenching rate constant were used to study the quenching mechanism; all results indicated that this quenching process was a static quenching process based on the formation of complex between nitrogen-doped graphene quantum dots and brilliant blue through hydrogen bond. Particularly, this method was used to quantitatively analyze the wine sample, of which results have a high consistence with the results of the spectrophotometric method; demonstrating this fluorescence quenching method could be used in practical sample application.

Hydrogen bonded dimers of ketocoumarin in the solid state and alcohol:water binary solvent: fluorescence spectroscopy, crystal structure and DFT investigation

2019 ◽  
Vol 43 (23) ◽  
pp. 9090-9105 ◽  
Author(s):  
Kannan Ramamurthy ◽  
E. J. Padma Malar ◽  
Chellappan Selvaraju
Keyword(s):  
Crystal Structure ◽  
Solid State ◽  
Binary Mixture ◽  
Fluorescence Spectroscopy ◽  
Emission Spectrum ◽  
Fluorescence Emission ◽  
Binary Solvent ◽  
Fluorescence Emission Spectrum ◽  
Hydrogen Bonded

Fluorescence emission spectrum of ketocoumarin dimers in an alcohol:water binary mixture and the solid state.

New insights into the binding behavior of lomefloxacin and human hemoglobin using biophysical techniques: binary and ternary approaches

2019 ◽  
Vol 43 (21) ◽  
pp. 8132-8145 ◽  
Author(s):  
Parisa Mokaberi ◽  
Vida Reyhani ◽  
Zeinab Amiri-Tehranizadeh ◽  
Mohammad Reza Saberi ◽  
Sima Beigoli ◽  
...  
Keyword(s):  
Absorption Spectrum ◽  
Energy Transfer ◽  
Emission Spectrum ◽  
High Probability ◽  
Fluorescence Emission ◽  
Fluorescence Emission Spectrum ◽  
Human Hemoglobin ◽  
Binding Behavior ◽  
Biophysical Techniques

Demonstrates the overlap that had been induced between the fluorescence emission spectrum of Hb and the absorption spectrum of drugs, which has proved that there is a high probability to the occurrence of energy transfer from Hb and LMF in the absence and presence of NRF.

Changes in the Fluorescence Emission Spectrum of Chlorella emersonii Induced by Cold Treatment; a Possible Regulative Feature of Energy Uptake

1982 ◽  
Vol 37 (5-6) ◽  
pp. 448-451 ◽  
Author(s):  
G. Harnischfeger ◽  
H. Jarry
Keyword(s):  
Emission Spectrum ◽  
Membrane Structure ◽  
Cold Treatment ◽  
Fluorescence Emission ◽  
Membrane Properties ◽  
Fluorescence Emission Spectrum ◽  
Isolated Chloroplasts

Abstract Algae, when slowly cooled down to around -5 °C, undergo a change in the fluorescence emission spectrum subsequently taken at liqu. nitrogen temperature. This change resembles the magnesium effect described by Murata [BBA 189,171 - 181, (1969)] for isolated chloroplasts. Evidence is shown, that both effects are indeed analogous. Cooling the organisms seems to in­ crease the permeability of the thylakoids for cations and, thus, a depletion with concomitant changes in membrane structure. The system serves as a model for the probable in vivo control of pigment interaction through alteration of membrane properties.

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