Structural basis for the anticoagulant activity of heparin. 2. Relationship of anticoagulant activity to the thermodynamics and fluorescence fading kinetics of acridine orange-heparin complexes

Biochemistry ◽  
1979 ◽  
Vol 18 (20) ◽  
pp. 4288-4292 ◽  
Author(s):  
Julian M. Menter ◽  
Robert E. Hurst ◽  
David A. Corliss ◽  
Seymour S. West ◽  
Edwin W. Abrahamson
Keyword(s):  
Acridine Orange ◽  
Anticoagulant Activity ◽  
Structural Basis ◽  
Heparin Complexes ◽  
Relationship Of ◽  
Kinetics Of

KINETICS OF FLUORESCENCE FADING OF ACRIDINE ORANGE-HEPARIN COMPLEXES IN SOLUTION

1978 ◽  
Vol 27 (5) ◽  
pp. 629-633 ◽  
Author(s):  
J. M. Menter ◽  
J. F. Golden ◽  
S. S. West
Keyword(s):  
Acridine Orange ◽  
Heparin Complexes ◽  
Kinetics Of

Heparin Excretion in Intact and Hepatectomized Rats

1981 ◽  
Vol 45 (02) ◽  
pp. 146-149
Author(s):  
Ray Losito ◽  
Harry Gattiker ◽  
Ginette Bilodeau
Keyword(s):  
Column Chromatography ◽  
Normal Values ◽  
Anticoagulant Activity ◽  
Biliary Fistula ◽  
Kinetics Of ◽  
Intact Rats

SummaryMetabolism and kinetics of 3H-heparin were compared in intact and hepatectomized rats. Rats were divided into three groups: 1) intact rats with biliary fistulas and cystostomies 2) intact rats with only cystostomies and 3) hepatectomized rats with cystostomies. Radioactivity in blood, bile and urine besides anticoagulant activity in blood and urine were examined. In addition, column chromatography of urine was used to isolate possible metabolites. Seventy percent and 80% of the radioactive dose was found in the urine of intact rats at 24 hr and 48 hr. Close to 5% of the radioactivity was found in bile or rats with a biliary fistula after 48 hr. The APTT declined to near normal values at 1 hr whether rats had a biliary fistula or not. In contrast, only 25 % of the radioactivity could be excreted into the urine of hepatectomized rats in 24 hr; the APTT did not decline as fast and at 5 hr, it was still 100 seconds. Only one radioactive component could be isolated on chromatography from all urines of these animals and appears to be similar to the original heparin. Thus, the liver has an important role to play in regulating the anticoagulant effects and excretion of heparin.

scholarly journals Reduction Mechanism of Fine Hematite Ore Particles in Suspension

2021 ◽  
Author(s):  
Zhiyuan Chen ◽  
Christiaan Zeilstra ◽  
Jan van der Stel ◽  
Jilt Sietsma ◽  
Yongxiang Yang
Keyword(s):  
Particle Size ◽  
Temperature Drop ◽  
Reduction Rate ◽  
Reduction Process ◽  
Reciprocal Relationship ◽  
Drop Tube ◽  
Surface Nucleation ◽  
Order Of Magnitude ◽  
Relationship Of ◽  
Kinetics Of

AbstractIn order to understand the pre-reduction behaviour of fine hematite particles in the HIsarna process, change of morphology, phase and crystallography during the reduction were investigated in the high temperature drop tube furnace. Polycrystalline magnetite shell formed within 200 ms during the reduction. The grain size of the magnetite is in the order of magnitude of 10 µm. Lath magnetite was observed in the partly reduced samples. The grain boundary of magnetite was reduced to molten FeO firstly, and then the particle turned to be a droplet. The Johnson-Mehl-Avrami-Kolmogorov model is proposed to describe the kinetics of the reduction process. Both bulk and surface nucleation occurred during the reduction, which leads to the effect of size on the reduction rate in the nucleation and growth process. As a result, the reduction rate constant of hematite particles increases with the increasing particle size until 85 µm. It then decreases with a reciprocal relationship of the particle size above 85 µm.

scholarly journals SIMILARITY OF THE KINETICS OF INVERTASE ACTION IN VIVO AND IN VITRO. II

1932 ◽  
Vol 16 (2) ◽  
pp. 233-242 ◽  
Author(s):  
B. G. Wilkes ◽  
Elizabeth T. Palmer
Keyword(s):  
Physiological Activity ◽  
Outer Region ◽  
Living Cells ◽  
Yeast Cells ◽  
Live Cells ◽  
Intracellular Enzyme ◽  
Relationship Of ◽  
Kinetics Of

1. The pH-activity relationship of invertase has been studied in vivo and in vitro under identical external environmental conditions. 2. The effect of changing (H+) upon the sucroclastic activity of living cells of S. cerevisiae and of invertase solutions obtained therefrom has been found, within experimental error, to be identical. 3. The region of living yeast cells in which invertase exerts its physiological activity changes its pH freely and to the same extent as that of the suspending medium. It is suggested that this may indicate that this intracellular enzyme may perform its work somewhere in the outer region of the cell. 4. In using live cells containing maltase, no evidence of increased sucroclastic activity around pH 6.9, due to the action of Weidenhagen's α-glucosidase (maltase), was found.

Application of Galvanic Zinc-Iron Coating in the Repair of Transport and Technological Machines

2021 ◽  
Vol 1022 ◽  
pp. 181-193
Author(s):  
Dmitry M. Rozhkov ◽  
Evgenia V. Eltoshkina ◽  
Petr I. Ilyin ◽  
Olga A. Svirbutovich
Keyword(s):  
Experimental Studies ◽  
Iron Alloy ◽  
Resource Saving ◽  
Experimental Base ◽  
Optimal Resource ◽  
Iron Coating ◽  
Saving Mode ◽  
Relationship Of ◽  
The Relationship ◽  
Kinetics Of

The article presents the results of experimental studies to determine the relationship between the electrolysis modes and the properties of electroplating coatings for mathematical modeling of the dynamics of the electrolytic process (MDEP), described by a system of ordinary differential equations due to the complex relationship of the kinetics of chemical reactions, hydrodynamics and mass transfer in the electrolyte flow, the kinematics of electrode plates, and the influence of the electric field of the "anode-cathode" pair on all these processes. At the same time, the experimental base was a series of full-scale experiments to restore the seats of the root supports of cylinder blocks with electroplated coatings. The final result of the research is the procedure for constructing an optimal resource-saving mode of electroplating, which is a zinc-iron alloy.

On the Mechanism of the Acridine Orange Sensitized Photodynamic Inactivation of Lysozyme

1976 ◽  
Vol 31 (1-2) ◽  
pp. 29-39 ◽  
Author(s):  
Peter Rosenkranz ◽  
I. Basic Kinetics ◽  
Hartmut Schmidt
Keyword(s):  
Singlet Oxygen ◽  
Acridine Orange ◽  
Ph Value ◽  
Photodynamic Inactivation ◽  
Rate Process ◽  
First Order ◽  
Quenching Process ◽  
Pseudo First Order ◽  
Kinetics Of ◽  
Excited Oxygen

The kinetics of the photodynamic desactivation of lysozyme in presence of acridine orange as the sensitizer have been investigated in detail varying oxygen, protein, dye concentration, ionic strength and pH value. The kinetics can be approximately described as an over all pseudo-first- order rate process. Changing the solvent from water to D2O or by quenching experiments in pres­ence of azide ions it could be shown that the desactivation of lysozyme is caused exclusively by singlet oxygen. The excited oxygen occurs via the triplet state of the dye with a rate constant considerably lower than that to be expected for a diffusionally controlled reaction. Singlet oxygen reacts chemically (desactivation, k=2.9 × 107 ᴍ-1 sec-1) and physically (quenching process, k = 4.1 × 108 ᴍ-1sec-1) with the enzyme. The kinetical analysis shows that additional chemical reactions between singlet oxygen and lysozyme would have only little influence on the kinetics of the desactivation as long as their products would be enzymatically active and their kinetical constants would be less than about 1 × 108 ᴍ-1 sec-1.

Comparison of the Chemical Reactivity and Bioactivity of Two Bioactive Glasses 47S6 and 48S4

2008 ◽  
Vol 396-398 ◽  
pp. 107-110 ◽  
Author(s):  
Hassane Oudadesse ◽  
M. Mami ◽  
R. Dorbez-Sridi ◽  
P. Pellen-Mussi ◽  
F. Perez ◽  
...  
Keyword(s):  
Mineral Composition ◽  
Chemical Reactivity ◽  
Rapid Quenching ◽  
Structural Basis ◽  
In Vitro Experiments ◽  
Layer Formation ◽  
Bioactive Glasses ◽  
Ionic Exchanges ◽  
Kinetics Of

This work is focused on the bioactive glasses obtained by melting and rapid quenching. Two glasses with mineral composition of: 47% SiO2 - 26% CaO - 21% Na2O - 6% P2O5 and 48% SiO2 - 30% CaO - 18% Na2O - 4% P2O5 were investigated. The aim of this study was to establish the kinetics of HCAp layer formation “in vitro” and to control the adhesion and proliferation cells of the two glasses in contact with osseous cells. Obtained results permit to evaluate their chemical reactivity and their bioactivity after immersion in the SBF-K9. Ionic exchanges between biomaterials and SBF liquid during the “in vitro” experiments highlight the differences of the chemical reactivity and bioactivity of 47S6 and 48S4. The structural basis for the effect of cristallinity on the rates of HCA formation in vitro in favour of glasses was also established. The melt derived 47S6 and 48S4 glasses offer to surgeons new compositions with different bioactivity kinetic that bioglassÒ 45S6 and can be adaptable in some other bony pathology.

Structural basis for the kinetics of Candida rugosa Lip1 and Lip3 isoenzymes

2002 ◽  
Vol 26 (1-2) ◽  
pp. 67-74 ◽  
Author(s):  
M Pernas ◽  
C López ◽  
A Prada ◽  
J Hermoso ◽  
M.L Rúa
Keyword(s):  
Candida Rugosa ◽  
Structural Basis ◽  
Kinetics Of

Understanding the quantitative structure–activity relationship of acetylcholinesterase inhibitors for the treatment of Alzheimer's disease

2015 ◽  
Vol 14 (06) ◽  
pp. 1550040 ◽  
Author(s):  
Anuradha Sharma ◽  
Poonam Piplani
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Quantitative Structure Activity Relationship ◽  
Structure Activity Relationship ◽  
Activity Relationship ◽  
Structural Basis ◽  
Quantitative Structure ◽  
Structure Activity ◽  
Qsar Models ◽  
Relationship Of

Alzheimer's disease (AD) is the most common cause of dementia in old aged people and clinically used drugs for treatment are associated with side effects. Thus, there is a current demand for the discovery and development of new potential molecules. However, the recent advances in drug therapy have challenged the predominance of the disease. In this manuscript, an attempt has been made to develop the 2D and 3D quantitative structure–activity relationship (QSAR) models for a series of rutaecarpine, quinazolines and 7,8-dehydrorutaecarpine derivatives to obtain insights to Acetylcholinesterase (AChE) inhibition. Five different QSAR models have been generated and validated using a set of 52 compounds comprising of varying scaffolds with IC50 values ranging from 11,000 nM to 0.6 nM. These AChE-specific prediction models (M1–M5) adequately reflect the structure–activity relationship of the existing AChE inhibitors. Out of all developed models, QSAR model generated using ADME properties has been found to be the best with satisfactory statistical significance (regression (r2) of 0.9309 and regression adjusted coefficient of variation [Formula: see text] of 0.9194). The QSAR models highlight the importance of aromatic moiety as their presence in the structure influence the biological activity. Additional insights on the compounds show that acyclic amines attached to side chain have lower activity than cyclic amines. The QSAR models pinpointing structural basis for the AChEIs suggest new guidelines for the design of novel molecules.

Sign in / Sign up

Export Citation Format

Share Document