scholarly journals Escherichia coli phosphoenolpyruvate dependent phosphotransferase system. NMR studies of the conformation of HPr and P-HPr and the mechanism of energy coupling

Biochemistry ◽  
1979 ◽  
Vol 18 (14) ◽  
pp. 2996-3001 ◽  
Author(s):  
G. Dooijewaard ◽  
F. F. Roossien ◽  
G. T. Robillard
Keyword(s):  
Escherichia Coli ◽  
Energy Coupling ◽  
Phosphotransferase System ◽  
Nmr Studies

scholarly journals The Ascorbate Transporter of Escherichia coli

2003 ◽  
Vol 185 (7) ◽  
pp. 2243-2250 ◽  
Author(s):  
Zhongge Zhang ◽  
Mohammad Aboulwafa ◽  
Meghan H. Smith ◽  
Milton H. Saier,
Keyword(s):  
Escherichia Coli ◽  
Gene Product ◽  
Energy Coupling ◽  
Phosphoryl Transfer ◽  
Transcriptional Level ◽  
Phosphotransferase System ◽  
Helical Segment ◽  
Enzyme I

ABSTRACT The sgaTBA genes of Escherichia coli encode a putative 12-transmembrane α-helical segment (12 TMS) transporter, an enzyme IIB-like protein and an enzyme IIA-like protein of the phosphotransferase system (PTS), respectively. We show that all three proteins as well as the energy-coupling PTS proteins, enzyme I and HPr, are required for the anaerobic utilization and uptake of l-ascorbate in vivo and its phosphoenolpyruvate-dependent phosphorylation in vitro. The transporter exhibits an apparent Km for l-ascorbate of 9 μM and is highly specific. The sgaTBA genes are regulated at the transcriptional level by the yjfQ gene product, as well as by Crp and Fnr. The yjfR gene product is essential for l-ascorbate utilization and probably encodes a cytoplasmic l-ascorbate 6-phosphate lactonase. We conclude that SgaT represents a novel prototypical enzyme IIC that functions with SgaA and SgaB to allow phosphoryl transfer from HPr(his-P) to l-ascorbate via the phosphoryl transfer pathway: PEP → enzyme I-P → HPr-P → IIA- \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(\stackrel{\mathrm{SgaA}}{\mathrm{P}}\) \end{document} → IIB- \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(\stackrel{\mathrm{SgaB}}{\mathrm{P}}\) \end{document} \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(\stackrel{\textstyle{\mathrm{IIC^{SgaT}}}}{{\rightarrow}}\) \end{document} l-ascorbate-6-P.

scholarly journals Energy Coupling in Membrane Vesicles of Escherichia coli

1974 ◽  
Vol 249 (9) ◽  
pp. 2939-2945
Author(s):  
Hajime Hirata ◽  
Karlheinz Altendorf ◽  
Franklin M. Harold
Keyword(s):  
Escherichia Coli ◽  
Membrane Vesicles ◽  
Energy Coupling

scholarly journals 31phospho-NMR demonstration of phosphocysteine as a catalytic intermediate on the Escherichia coli phosphotransferase system EIIMtl.

1991 ◽  
Vol 266 (11) ◽  
pp. 6690-6692
Author(s):  
H H Pas ◽  
G H Meyer ◽  
W H Kruizinga ◽  
K S Tamminga ◽  
R P van Weeghel ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Phosphotransferase System
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