Interaction of [125I]-.alpha.-bungarotoxin with acetylcholine receptor from Torpedo californica

Biochemistry ◽  
1979 ◽  
Vol 18 (10) ◽  
pp. 1875-1883 ◽  
Author(s):  
Steven G. Blanchard ◽  
Ulrich Quast ◽  
Kenton Reed ◽  
Theodore Lee ◽  
Michael I. Schimerlik ◽  
...  
Keyword(s):  
Acetylcholine Receptor ◽  
Torpedo Californica ◽  
Alpha Bungarotoxin

scholarly journals Profile of the α-bungarotoxin-binding regions on the extracellular part of the α-chain of Torpedo californica acetylcholine receptor

1987 ◽  
Vol 248 (3) ◽  
pp. 847-852 ◽  
Author(s):  
B MULAC-JERIČEVIČ ◽  
M Z Atassi
Keyword(s):  
Acetylcholine Receptor ◽  
Torpedo Californica ◽  
Alpha Chain ◽  
Toxin Binding ◽  
The Third ◽  
Binding Regions ◽  
Α Chain ◽  
Alpha Bungarotoxin ◽  
Receptor Antibodies ◽  
Anti Acetylcholine

The continuous alpha-neurotoxin-binding regions on the extracellular part (residues 1-210) of the alpha-chain of Torpedo californica acetylcholine receptor were localized by reaction of 125I-labelled alpha-bungarotoxin with synthetic overlapping peptides spanning this entire part of the chain. The specificity of the binding was confirmed by inhibition with unlabelled toxin and, for appropriate peptides, with unlabelled anti-(acetylcholine receptor) antibodies. Five toxin-binding regions were localized within residues 1-10, 32-41, 100-115, 122-150 and 182-198. The third, fourth and fifth (and to a lesser extent the first and second) toxin-binding regions overlapped with regions recognized by anti-(acetylcholine receptor) antibodies. The five toxin-binding regions may be distinct sites or, alternatively, different ‘faces’ in one (or more) sites.

Uses of fluorescent cholinergic analogs to study binding sites for cholinergic ligands in Torpedo californica acetylcholine receptor

Biochemistry ◽  
1979 ◽  
Vol 18 (10) ◽  
pp. 1855-1861 ◽  
Author(s):  
Jurgen Bode ◽  
Terry Moody ◽  
Michael Schimerlik ◽  
Michael Raftery
Keyword(s):  
Acetylcholine Receptor ◽  
Binding Sites ◽  
Torpedo Californica
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