Metabolism of low molecular weight ribonucleic acids in early sea urchin embryos

Biochemistry ◽  
1979 ◽  
Vol 18 (7) ◽  
pp. 1353-1360 ◽  
Author(s):  
Parmod Nijhawan ◽  
William F. Marzluff
Keyword(s):  
Molecular Weight ◽  
Sea Urchin ◽  
Low Molecular Weight ◽  
Ribonucleic Acids ◽  
Sea Urchin Embryos

Isolation of Homologous Nuclear Dnas from Sea-Urchin Embryos

1972 ◽  
Vol 11 (1) ◽  
pp. 153-171
Author(s):  
R. F. BAKER
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Sea Urchin ◽  
Dna Hybridization ◽  
Nuclear Dna ◽  
Strongylocentrotus Purpuratus ◽  
Low Molecular Weight ◽  
Blastula Stage ◽  
Sea Urchin Embryos

Studies are presented on the ability of low-molecular-weight nuclear DNA fractions to hybridize to higher-molecular-weight nuclear DNAs taken from different stages of early developing sea-urchin embryos, Strongylocentrotus purpuratus. Using preparative DNA-DNA hybridization, a fraction of 6o-s mid-blastula DNA was isolated for its ability to anneal to 10-s morula DNA. Approximately 80 regions on each 6o-s molecule were found to be homologous to 10-s DNA. High-molecular-weight (> 240-s) nuclear DNA from mesenchyme blastula stage and later stages (compared with DNA from pre-blastula nuclei) shows an increase in the number of regions homologous to fractions of 10- and 60-s DNAs.

Small molecular weight RNA components in sea urchin embryos

1973 ◽  
Vol 78 (2) ◽  
pp. 287-294 ◽  
Author(s):  
S. Frederiksen ◽  
P. Hellung-Larsen ◽  
J. Engberg
Keyword(s):  
Molecular Weight ◽  
Sea Urchin ◽  
Small Molecular Weight ◽  
Sea Urchin Embryos

scholarly journals Increased synthesis of low-molecular-weight nuclear ribonucleic acids of rat liver after γ irradiation and hepatectomy

1975 ◽  
Vol 146 (2) ◽  
pp. 357-360 ◽  
Author(s):  
A Fónagy ◽  
E J Hidvégi
Keyword(s):  
Molecular Weight ◽  
Partial Hepatectomy ◽  
Rat Liver ◽  
Orotic Acid ◽  
Low Molecular Weight ◽  
Polyacrylamide Gels ◽  
Γ Irradiation ◽  
Ribonucleic Acids

Incorporation of [3H]orotic acid into low-molecular-weight nRNA of rat liver, fractionated on polyacrylamide gels, increased 6-12h after partial hepatectomy and 6h after γ-irridation at 2000 R. The incorporation of orotic acid was particularly increased into the 4.5S, 5S and approx. 10S nRNA fractions. If the irradiation was given after 6h of regeneration and RNA was isolated from the nucleus 12h after hepatectomy then the incorporation of orotic acid into these low-molecular-weight nRNA components was greater than after hepatectomy or irradiation alone.

The role of neurohumors in early embryogenesis

Development ◽  
1968 ◽  
Vol 20 (1) ◽  
pp. 119-128
Author(s):  
G. A. Buznikov ◽  
I. V. Chudakova ◽  
L. V. Berdysheva ◽  
N. M. Vyazmina
Keyword(s):  
Molecular Weight ◽  
Xenopus Laevis ◽  
Sea Urchin ◽  
Early Embryogenesis ◽  
Low Molecular Weight ◽  
Insect Eggs ◽  
Insect Embryos ◽  
Related Compounds ◽  
Xenopus Laevis Embryos

In a previous paper (Buznikov, Chudakova & Zvezdina, 1964) it has been reported that serotonin (5-hydroxytryptamine, 5-HT) may be involved in early embryogenesis in various groups of animals. This conclusion was confirmed by Baker's recent publication (Baker, 1965) concerning 5-HT synthesis in Xenopus laevis embryos. Some other low molecular weight substances, neurohumors or related compounds, are known to be synthesized in fertilized eggs as well. Acetylcholine (ACh) synthesis in sea-urchin eggs and embryos was demonstrated by Numanoi (1953, 1955, 1959, 1961). It seems possible that ACh can be synthesized in fertilized insect eggs as well (Morley & Schachter, 1963; Schachter, 1964). The synthesis of another neurohumor, dopamine (DA), in early insect embryos seems to be indisputable (Furneaux & McFarlane, 1965). However, in most cases changes in the level of neurohumors with age have not been studied. In the present paper data concerning the change of concentration with age of ACh and catecholamines (adrenaline (A) and noradrenaline (NA)) in early sea-urchin embryos will be presented.

Multiple Forms of Potato Spindle-Tuber Metavirus Ribonucleic Acid

1974 ◽  
Vol 52 (9) ◽  
pp. 809-812 ◽  
Author(s):  
R. P. Singh ◽  
J. J. Michniewicz ◽  
S. A. Narang
Keyword(s):  
Molecular Weight ◽  
Ribonucleic Acid ◽  
Gel Filtration ◽  
Low Molecular Weight ◽  
Multiple Forms ◽  
Ribonucleic Acids

Multiple infectious low-molecular weight ribonucleic acids from Scopolia sinensis plants, infected with potato spindle tuber metavirus were isolated by Bio-Gel P-200 gel filtration columns and the slowest-moving form (infectious peak III) was eluted with bulk of 4 S-RNA.

scholarly journals Anticoagulant and Antithrombotic Properties of Three Structurally Correlated Sea Urchin Sulfated Glycans and Their Low-Molecular-Weight Derivatives

Marine Drugs ◽  
2018 ◽  
Vol 16 (9) ◽  
pp. 304 ◽  
Author(s):  
Ariana Vasconcelos ◽  
Isabela Sucupira ◽  
Alessandra Guedes ◽  
Ismael Queiroz ◽  
Flavia Frattani ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Sea Urchin ◽  
Factor Xa ◽  
Activate Factor ◽  
Low Molecular Weight ◽  
Factor Xii ◽  
Sulfated Galactan ◽  
Sulfated Fucan

The anticoagulant and antithrombotic properties of three structurally correlated sea urchin-derived 3-linked sulfated α-glycans and their low molecular-weight derivatives were screened comparatively through various in vitro and in vivo methods. These methods include activated partial thromboplastin time, the inhibitory activity of antithrombin over thrombin and factor Xa, venous antithrombosis, the inhibition of platelet aggregation, the activation of factor XII, and bleeding. While the 2-sulfated fucan from Strongylocentrotus franciscanus was observed to be poorly active in most assays, the 4-sulfated fucan from Lytechinus variegatus, the 2-sulfated galactan from Echinometra lucunter and their derivatives showed multiple effects. All marine compounds showed no capacity to activate factor XII and similar low bleeding tendencies regardless of the dose concentrations used to achieve the highest antithrombotic effect observed. The 2-sulfated galactan showed the best combination of results. Our work improves the background about the structure-function relationship of the marine sulfated glycans in anticoagulation and antithrombosis. Besides confirming the negative effect of the 2-sulfated fucose and the positive effect of the 2-sulfated galactose on anticoagulation in vitro, our results also demonstrate the importance of this set of structural requirements on antithrombosis in vivo, and further support the involvement of high-molecular weight and 4-sulfated fucose in both activities.

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