Correlations between the duplex winding angle and the circular dichroism spectrum of calf thymus DNA

Biochemistry ◽  
1979 ◽  
Vol 18 (1) ◽  
pp. 84-91 ◽  
Author(s):  
Aurelia Chan ◽  
Robert Kilkuskie ◽  
Sue Hanlon
Keyword(s):  
Circular Dichroism ◽  
Circular Dichroism Spectrum ◽  
Calf Thymus Dna ◽  
Calf Thymus ◽  
Winding Angle ◽  
Circular Dichroïsm

Circular Dichroism and Ultraviolet Absorbance of Calf Thymus DNA in Presence of CH3HgOH

1979 ◽  
Vol 34 (3-4) ◽  
pp. 259-265 ◽  
Author(s):  
Michael S. Clegg ◽  
Dieter W. Gruenwedel
Keyword(s):  
Circular Dichroism ◽  
Absorption Band ◽  
Calf Thymus Dna ◽  
Absorbance Spectrum ◽  
Absorption Bands ◽  
Long Wavelength ◽  
Ultraviolet Absorbance ◽  
Rotational Strength ◽  
Calf Thymus ◽  
Circular Dichroïsm

Abstract The changes that one observes upon the addition of CH3HgOH in the circular dichroism spec­trum and ultraviolet absorbance spectrum of native calf thymus DNA, dissolved in buffered (pH 6.8) solutions of Na2SO4 at pNa 2.0, 1.5, 1.0, and 0.0, respectively (pNa = - log [Na+]), are shown to be due to denaturation brought about by the organomercurial interacting with the base moieties of the polymer. The changes are characterized by an extensive shift of both spectra to longer wavelengths, by a decrease of the rotational strength of the long-wavelength positive dichroic absorption band, and by an increase in the UV absorbance at λmax . Both the hyperchromicity Hλ of calf thymus DNA and the normalized decrease of the rotational strength of its long-wavelength positive dichromic band, Ω , display cooperativity when plotted against the methylmercury concentration pM (pM = - log [CH3HgOH]added) at a given salt strength. Rotational strength data, evaluated by integration of the area under the positive and negative dichroic absorption bands, have been tabulated for selected values of pNa and pM. They are compared with data available from the literature. In absence of CH3HgOH, and with varying salt strength, native calf thymus DNA exhibits alterations in the long-wavelength positive dichroic absorption band that are interpreted as representing B → C transitions in agreement with currently held views regarding their origin. Similar salt-induced alterations have been noted in the case of denatured DNA; their meaning in terms of DNA geometry remains unclear at this point in time.

Synthesis, crystal structure, DNA-binding and magnetism of copper 15-metallacrown-5 complexes based on glycinehydroxamic acid ligand

RSC Advances ◽  
2016 ◽  
Vol 6 (53) ◽  
pp. 47196-47202 ◽  
Author(s):  
Yanxia Meng ◽  
Hua Yang ◽  
Dacheng Li ◽  
Suyuan Zeng ◽  
Guifang Chen ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Circular Dichroism ◽  
Dna Binding ◽  
Fluorescence Spectroscopy ◽  
Calf Thymus Dna ◽  
Binding Properties ◽  
Acid Ligand ◽  
Calf Thymus ◽  
Dna Binding Properties ◽  
Circular Dichroïsm

Six lanthanide 15-MC-5 complexes based on glycinehydroxamic acid were synthesized. DNA-binding properties of the six complexes with calf thymus DNA were investigated using fluorescence spectroscopy and circular dichroism studies.

Water-soluble lysine-containing polypeptides. V. Circular dichroisrm, electron microscopic, and thermal denaturation studies of the interaction of poly(L-lysyl-L-alanine) and poly(L-lysyl-D-alanine) with calf thymus DNA

1978 ◽  
Vol 56 (20) ◽  
pp. 2650-2656 ◽  
Author(s):  
Sandra L. Kielland ◽  
Lewis A. Slotin ◽  
Ross E. Williams
Keyword(s):  
Circular Dichroism ◽  
Secondary Structure ◽  
Electron Micrographs ◽  
Thermal Denaturation ◽  
Water Soluble ◽  
Calf Thymus Dna ◽  
Circular Dichroism Spectra ◽  
Electron Microscopic ◽  
Calf Thymus ◽  
Circular Dichroïsm

Turbidity measurements, circular dichroism spectra, electron micrographs and thermal denaturation profiles of complexes between sonicated DNA and either poly(L-lysyl-L-alanine) or poly(L-lysyl-D-alanine) show significant differences which might be related to the primary or secondary structure of the polypeptides.

scholarly journals Multi-Spectroscopic and Molecular Simulation Approaches to Characterize the Intercalation Binding of 1-Naphthaleneacetic Acid With Calf Thymus DNA

2021 ◽  
Vol 3 ◽  
Author(s):  
Xing Hu ◽  
Xiaoqiao Luo ◽  
Zhisheng Zhou ◽  
Rui Wang ◽  
Yaqin Hu ◽  
...  
Keyword(s):  
Circular Dichroism ◽  
Molecular Simulation ◽  
The Body ◽  
Calf Thymus Dna ◽  
Naphthaleneacetic Acid ◽  
Visible Absorption ◽  
Peak Intensity ◽  
Calf Thymus ◽  
Endogenous Fluorescence ◽  
Circular Dichroïsm

1–Naphthaleneacetic acid (NAA), having high-quality biological activity and great yield-increasing potential in agricultural production, is a broad-spectrum plant growth regulator. Although NAA is of low toxicity, it can affect the balance of the human metabolism and damage the body if it is used in high quantity for a long time. In this study, the interaction of NAA with calf thymus DNA (ctDNA) was investigated under simulated human physiological acidity (pH 7.4) using fluorescence, ultraviolet-visible absorption, and circular dichroism spectroscopy combined with viscosity measurements and molecular simulation techniques. The quenching of the endogenous fluorescence of NAA by ctDNA, observed in the fluorescence spectrum experiment, was a mixed quenching process that mainly resulted from the formation of the NAA–ctDNA complex. NAA mainly interacted with ctDNA through hydrophobic interaction, and the binding constant and quenching constant at room temperature (298 K) were 0.60 × 105 L mol−1 and 1.58 × 104 L mol−1, respectively. Moreover, the intercalation mode between NAA and ctDNA was verified in the analysis of melting point, KI measurements, and the viscosity of ctDNA. The results were confirmed by molecular simulation, and it showed that NAA was enriched near the C–G base of ctDNA. As shown in circular dichroism spectra, the positive peak intensity of ctDNA intensified along with a certain degree of redshift, while the negative peak intensity decreased after binding with NAA, suggesting that the binding of NAA induced the transformation of the secondary structure of ctDNA from B-form to A-form. These researches will help to understand the hazards of NAA to the human body more comprehensively and concretely, to better guide the use of NAA in industry and agriculture.

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