Reactions of 2-thioribothymidine and 4-thiouridine with hydrogen peroxide in transfer ribonucleic acids from Thermus thermophilus and Escherichia coli as studied by circular dichroism

Biochemistry ◽  
1980 ◽  
Vol 19 (24) ◽  
pp. 5542-5549 ◽  
Author(s):  
Kimitsuna Watanabe
Keyword(s):  
Escherichia Coli ◽  
Hydrogen Peroxide ◽  
Circular Dichroism ◽  
Thermus Thermophilus ◽  
Ribonucleic Acids ◽  
Circular Dichroïsm

scholarly journals The circular dichroism of ribosomal ribonucleic acids

1976 ◽  
Vol 155 (2) ◽  
pp. 279-291 ◽  
Author(s):  
R A. Cox ◽  
W Hirst ◽  
E Godwin ◽  
I Kaiser
Keyword(s):  
Escherichia Coli ◽  
Circular Dichroism ◽  
Frame Of Reference ◽  
Partial Hydrolysis ◽  
Ribonucleic Acids ◽  
Helical Content ◽  
Rrna Species ◽  
The Difference ◽  
Hydrolysis Of ◽  
Circular Dichroïsm

1. The c.d. (circular dichroism) of Drosophila melanogaster rRNA (42% G+C) and of G+C-rich fragments (78% G+C) obtained by partial hydrolysis of rabbit L-rRNA (the largest RNA species isolated from the large subribosomal particle) were measured and found to differ substantially. 2. To interpret these spectra a relation between c.d. of bihelical RNA and % G+C was derived, namely delta epsilonfG = AFG2+bfG+c, where deltaepsilonfG is the c.d. of RNA characterized by a mole fraction, fG, of guanine nucleotides and a, b and c are constants. 3. A frame of reference was established by studying the c.d. of a range of rRNA species, including S-rRNA (the RNA species isolated from the smaller subribosomal particle) and L-rRNA of Escherichia coli. 4. It was found for the rRNA species studied that 0.60+/-0.05 of residues appear to form bihelical secondary structure. 5. A higher helical content, 0.66+/-0.05, was found for the G+C-rich fragment of L-rRNA. The difference in the c.d. of rabbit L-rRNA and of D. melanogaster rRNA is attributable to the dependence of c.d. of the bihelical parts on %G+C. 6. The minimum in c.d. at 295 nm increases with increasing %G+C. The c.d. of rRNA was compared with that of the parent subparticle in this region of the spectrum, where high precision may be attained.

scholarly journals Circular dichroism investigation of Escherichia coli adenylate kinase.

1987 ◽  
Vol 262 (6) ◽  
pp. 2502-2506
Author(s):  
M. Monnot ◽  
A.M. Gilles ◽  
I.S. Girons ◽  
S. Michelson ◽  
O. Bârzu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Circular Dichroism ◽  
Adenylate Kinase ◽  
Circular Dichroïsm

Folding Pathway of Escherichia coli Ribonuclease HI: A Circular Dichroism, Fluorescence, and NMR Study

Biochemistry ◽  
1995 ◽  
Vol 34 (51) ◽  
pp. 16552-16562 ◽  
Author(s):  
Kazuhiko Yamasaki ◽  
Kyoko Ogasahara ◽  
Katsuhide Yutani ◽  
Motohisa Oobatake ◽  
Shigenori Kanaya
Keyword(s):  
Escherichia Coli ◽  
Circular Dichroism ◽  
Folding Pathway ◽  
Nmr Study ◽  
Circular Dichroïsm

Physical studies on the ribosomal "A" protein from two archaebacteria, Halobacterium cutirubrum and Methanobacterium thermoautotrophicum

1984 ◽  
Vol 62 (1) ◽  
pp. 44-48 ◽  
Author(s):  
A. T. Gudkov ◽  
S. Yu Venyaminov ◽  
A. T. Matheson
Keyword(s):  
Escherichia Coli ◽  
Circular Dichroism ◽  
Secondary Structure ◽  
Quaternary Structure ◽  
Methanobacterium Thermoautotrophicum ◽  
Sedimentation Equilibrium ◽  
Effect Of Temperature ◽  
Extreme Halophile ◽  
Moderate Halophile ◽  
Circular Dichroïsm

Physical studies on the effect of temperature and ionic conditions on the secondary, tertiary, and quaternary structure of the ribosomal "A" protein, equivalent to L7/L12 in Escherichia coli, from two archaebacteria were performed using circular dichroism and sedimentation equilibrium measurements. The two archaebacteria investigated were Halobacterium cutirubrum, an extreme halophile, and Methanobacterium thermoautotrophicum, a thermophile which also showed properties of a moderate halophile. The changes in the secondary structure and the thermostability of these proteins were directly related to the internal salt concentrations of the two archaebacteria. At the higher salt concentrations the changes in the secondary structure resulted in changes in the tertiary and quaternary structure of these proteins.

Infrared and vibrational circular dichroism intensities of model systems CH3OH, CH3NH2, NH2NH2, NH2OH, and HOOH and the deuterated species, ND2ND2, DOOH, and DOOD: A theoretical study using the vibronic coupling formalism

1990 ◽  
Vol 68 (2) ◽  
pp. 258-266 ◽  
Author(s):  
Arvi Rauk ◽  
Remo Dutler ◽  
Danya Yang
Keyword(s):  
Hydrogen Peroxide ◽  
Circular Dichroism ◽  
Vibronic Coupling ◽  
Vibrational Circular Dichroism ◽  
Model Systems ◽  
Antisymmetric Mode ◽  
Coupled Oscillator ◽  
Torsional Mode ◽  
Rotational Strength ◽  
Circular Dichroïsm

We have implemented at the abinitio level the vibronic coupling formalism for infrared and vibrational circular dichroism (VCD) intensities of Nafie and Freedman. We report here the results of a series of calculations on model systems. Hydrazine and hydrogen peroxide exist in chiral conformations. Although low barriers to racemization preclude the possibility of optical resolution of the parent compounds, the local gauche configuration at the heteroatom bond is preserved in substituted hydrazines and peroxides and these chiral fragments may contribute significantly to the vibrational chiroptical properties of the molecules in which they appear. The calculated VCD rotational strengths for the chiral gauche forms of both molecules suggest that the torsional mode is very intense in the VCD spectrum. In gauche hydrazine of P chirality, the in- and out-of-phase umbrella motions of the nitrogen atoms, ν6 and ν12, respectively, give rise to an intense CD couplet with negative rotational strength to the long wave length side. In hydrazine, all combinations of NH stretches are relatively weak in the IR but the highest frequency mode of b symmetry, ν8, has moderate positive strength. The in- and out-of-phase OOH bends of HOOH behave as a coupled oscillator system, exhibiting a strong bisignate CD pattern. The signals calculated for the OH stretches are not consistent with a coupled oscillator model. The nearly degenerate stretches result in a single band arising from the more intense negative rotational strength of the antisymmetric mode ν5, in HOOH of P chirality. An alternative model, based on induced charge flow along the chirally disposed skeleton (i.e., Nafie and Freedman's 3-bond electronic current model), explains the calculated sign of the antisymmetric mode, ν5. Significant changes in the VCD patterns due to deuterium substitution in both chiral molecules are discussed. Keywords: vibrational circular dichroism (VCD), abinitio, infrared intensities, molecular orbital, vibronic coupling theory, methanol, methanamine, hydrazine, hydroxylamine, hydrogen peroxide.

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