Deuterium isotope effects on lactate dehydrogenase using L-2-hydroxysuccinamate and effect of an inhibitor in the variable substrate on observed isotope effects

Biochemistry ◽  
1980 ◽  
Vol 19 (14) ◽  
pp. 3153-3157 ◽  
Author(s):  
C. E. Grimshaw ◽  
W. W. Cleland
Keyword(s):  
Lactate Dehydrogenase ◽  
Isotope Effects ◽  
Deuterium Isotope Effects ◽  
Variable Substrate

Mechanism of hydration and isomerisation of 4-ethylidene-2,6-di-tert-butyl-2,5-cyclohexadien-1-one. Kinetics, acid-base catalysis and solvent deuterium isotope effects

1979 ◽  
Vol 44 (1) ◽  
pp. 110-122 ◽  
Author(s):  
Jiří Velek ◽  
Bohumír Koutek ◽  
Milan Souček
Keyword(s):  
Aqueous Medium ◽  
Rate Equation ◽  
Kinetic Data ◽  
Isotope Effects ◽  
Base Catalysis ◽  
Quinone Methide ◽  
Reaction Products ◽  
Acid Base ◽  
Deuterium Isotope Effects ◽  
Hydroxybenzyl Alcohol

Competitive hydration and isomerisation of the quinone methide I at 25 °C in an aqueous medium in the region of pH 2.4-13.0 was studied spectrophotometrically. The only reaction products in the studied range of pH are 4-hydroxybenzyl alcohol (II) and 4-hydroxystyrene (III). The form of the overall rate equation corresponds to a general acid-base catalysis. The mechanism of both reactions for three markedly separated pH regions is discussed on the basis of kinetic data and solvent deuterium effect.

Sign in / Sign up

Export Citation Format

Share Document