Solubilization and characterization of apolipoprotein B from human serum low-density lipoprotein in n-dodecyl octaethylene glycol monoether

Biochemistry ◽  
1980 ◽  
Vol 19 (8) ◽  
pp. 1593-1598 ◽  
Author(s):  
Robert M. Watt ◽  
Jacqueline A. Reynolds
Keyword(s):  
Human Serum ◽  
Apolipoprotein B ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density

scholarly journals Characterization of Low- and Very-Low-Density Hepatitis C Virus RNA-Containing Particles

2002 ◽  
Vol 76 (14) ◽  
pp. 6919-6928 ◽  
Author(s):  
P. André ◽  
F. Komurian-Pradel ◽  
S. Deforges ◽  
M. Perret ◽  
J. L. Berland ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Apolipoprotein B ◽  
Core Protein ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Hcv Rna ◽  
Low Density ◽  
Density Fractions

ABSTRACT The presence of hepatitis C virus (HCV) RNA-containing particles in the low-density fractions of plasma has been associated with high infectivity. However, the nature of circulating HCV particles and their association with immunoglobulins or lipoproteins as well as the characterization of cell entry have all been subject to conflicting reports. For a better analysis of HCV RNA-containing particles, we quantified HCV RNA in the low-density fractions of plasma corresponding to the very-low-density lipoprotein (VLDL), intermediate-density lipoprotein, and low-density lipoprotein (LDL) fractions from untreated chronically HCV-infected patients. HCV RNA was always found in at least one of these fractions and represented 8 to 95% of the total plasma HCV RNA. Surprisingly, immunoglobulins G and M were also found in the low-density fractions and could be used to purify the HCV RNA-containing particles (lipo-viro-particles [LVP]). Purified LVP were rich in triglycerides; contained at least apolipoprotein B, HCV RNA, and core protein; and appeared as large spherical particles with a diameter of more than 100 nm and with internal structures. Delipidation of these particles resulted in capsid-like structures recognized by anti-HCV core protein antibody. Purified LVP efficiently bind and enter hepatocyte cell lines, while serum or whole-density fractions do not. Binding of these particles was competed out by VLDL and LDL from noninfected donors and was blocked by anti-apolipoprotein B and E antibodies, whereas upregulation of the LDL receptor increased their internalization. These results suggest that the infectivity of LVP is mediated by endogenous proteins rather than by viral components providing a mechanism of escape from the humoral immune response.

scholarly journals Characterization of apolipoprotein B from human serum low density lipoprotein in n-dodecyl octaethyleneglycol monoether: an electron microscope study.

1980 ◽  
Vol 87 (3) ◽  
pp. 555-561 ◽  
Author(s):  
G Zampighi ◽  
J A Reynolds ◽  
R M Watt
Keyword(s):  
Apolipoprotein B ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Serum Lipoprotein ◽  
Low Density ◽  
Apo B ◽  
Shaped Particle ◽  
Orderly Fashion ◽  
High Degree

We have studied the structure of the totally delipidated polypeptide (apolipoprotein B [apo B]) present in low-density serum lipoprotein in detergent (n-dodecyl octaethyleneglycol monoether) solution by electron microscopy. The protein-detergent complex appears as a rod-shaped particle, 75-80 nm long and 4.5-5.5 nm wide. The volume of this particle is consistent with the previously published composition reported by Watt and Reynolds (1980, Biochemistry 19:1593-1598) of two copies of apo B and five to six equivalent micelles of detergent. The asymmetric particle possesses a high degree of flexibility and a strong tendency to self-associate in an orderly fashion. The extent of this association is pH dependent.

Purification and characterization of low density lipoprotein containing apolipoprotein B-48 from the plasma of an apoE-deficient patient

1997 ◽  
Vol 259 (1-2) ◽  
pp. 109-116
Author(s):  
Yoshiaki Hashimoto ◽  
Kazuhisa Tsukamoto ◽  
Tamio Teramoto ◽  
Kiyoshi Kurokawa ◽  
Tsuyoshi Watanabe
Keyword(s):  
Apolipoprotein B ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density ◽  
Purification And Characterization ◽  
Deficient Patient

scholarly journals The Surface-Exposed, Trypsin-Accessible Segments of Apolipoprotein B in the Low-Density Lipoprotein of Human Serum

1982 ◽  
Vol 125 (3) ◽  
pp. 479-489 ◽  
Author(s):  
M. John CHAPMAN ◽  
Anne MILLET ◽  
Dominique LAGRANGE ◽  
Sonia GOLDSTEIN ◽  
Yves BLOUQUIT ◽  
...  
Keyword(s):  
Human Serum ◽  
Apolipoprotein B ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density
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