Extensive homology of nuclear ribonucleic acid and polysomal poly(adenylic acid) messenger ribonucleic acid between normal and neoplastically transformed cells

Biochemistry ◽  
1980 ◽  
Vol 19 (5) ◽  
pp. 821-832 ◽  
Author(s):  
Robert K. Moyzis ◽  
Deborah L. Grady ◽  
David W. Li ◽  
Stuart E. Mirvis ◽  
Paul O. P. Ts'o
Keyword(s):  
Ribonucleic Acid ◽  
Transformed Cells ◽  
Messenger Ribonucleic Acid ◽  
Adenylic Acid

Post-translational regulation of the 54K cellular tumor antigen in normal and transformed cells

1981 ◽  
Vol 1 (2) ◽  
pp. 101-110
Author(s):  
M Oren ◽  
W Maltzman ◽  
A J Levine
Keyword(s):  
Tumor Antigen ◽  
Ribonucleic Acid ◽  
Translational Regulation ◽  
Simian Virus 40 ◽  
T Antigen ◽  
Transformed Cells ◽  
Permissive Temperature ◽  
3T3 Cells ◽  
Messenger Ribonucleic Acid

The 54K cellular tumor antigen has been translated in vitro, using messenger ribonucleic acids from simian virus 40 (SV40)-transformed cells or 3T3 cells. The in vitro 54K product could be immunoprecipitated with SV40 tumor serum and had a peptide map that was similar, but not identical, to the in vivo product. The levels of this 54K protein in SV3T3 cells were significantly higher than those detected in 3T3 cells (D. I. H. Linzer, W. Maltzman, and A. J. Levine, Virology 98:308-318, 1979). In spite of this, the levels of translatable 54K messenger ribonucleic acid from 3T3 and SV3T3 cells were roughly equivalent or often greater in 3T3 cells. Pulse-chase experiments with the 54K protein from 3T3 or SV3T3 cells demonstrated that this protein, once synthesized, was rapidly degraded in 3T3 cells but was extremely stable in SV3T3 cells. Similarly, in an SV40 tsA-transformed cell line, temperature sensitive for the SV40 T-antigen, the 54K protein was rapidly turned over at the nonpermissive temperature and stable at the permissive temperature, whereas the levels of translatable 54K messenger ribonucleic acid at each temperature were roughly equal. These results demonstrate a post-translational regulation of the 54K cellular tumor antigen and suggest that this control is mediated by the SV40 large T-antigen.

Translation of mouse globin messenger ribonucleic acid from which the poly(adenylic acid) sequence has been removed

Biochemistry ◽  
1974 ◽  
Vol 13 (4) ◽  
pp. 703-707 ◽  
Author(s):  
Robert Williamson ◽  
Jennifer Crossley ◽  
Stephen Humphries
Keyword(s):  
Ribonucleic Acid ◽  
Messenger Ribonucleic Acid ◽  
Adenylic Acid

scholarly journals Post-translational regulation of the 54K cellular tumor antigen in normal and transformed cells.

1981 ◽  
Vol 1 (2) ◽  
pp. 101-110 ◽  
Author(s):  
M Oren ◽  
W Maltzman ◽  
A J Levine
Keyword(s):  
Tumor Antigen ◽  
Ribonucleic Acid ◽  
Translational Regulation ◽  
Simian Virus 40 ◽  
T Antigen ◽  
Transformed Cells ◽  
Permissive Temperature ◽  
3T3 Cells ◽  
Messenger Ribonucleic Acid

The 54K cellular tumor antigen has been translated in vitro, using messenger ribonucleic acids from simian virus 40 (SV40)-transformed cells or 3T3 cells. The in vitro 54K product could be immunoprecipitated with SV40 tumor serum and had a peptide map that was similar, but not identical, to the in vivo product. The levels of this 54K protein in SV3T3 cells were significantly higher than those detected in 3T3 cells (D. I. H. Linzer, W. Maltzman, and A. J. Levine, Virology 98:308-318, 1979). In spite of this, the levels of translatable 54K messenger ribonucleic acid from 3T3 and SV3T3 cells were roughly equivalent or often greater in 3T3 cells. Pulse-chase experiments with the 54K protein from 3T3 or SV3T3 cells demonstrated that this protein, once synthesized, was rapidly degraded in 3T3 cells but was extremely stable in SV3T3 cells. Similarly, in an SV40 tsA-transformed cell line, temperature sensitive for the SV40 T-antigen, the 54K protein was rapidly turned over at the nonpermissive temperature and stable at the permissive temperature, whereas the levels of translatable 54K messenger ribonucleic acid at each temperature were roughly equal. These results demonstrate a post-translational regulation of the 54K cellular tumor antigen and suggest that this control is mediated by the SV40 large T-antigen.

Length heterogeneity in the poly(adenylic acid) region of yeast messenger ribonucleic acid

Biochemistry ◽  
1974 ◽  
Vol 13 (26) ◽  
pp. 5378-5383 ◽  
Author(s):  
Bernd Groner ◽  
Nancy Hynes ◽  
Stephen Phillips
Keyword(s):  
Ribonucleic Acid ◽  
Messenger Ribonucleic Acid ◽  
Adenylic Acid ◽  
Acid Region
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