Biosynthesis of interstitial types of collagen by albumin-producing rat liver parenchymal cell (hepatocyte) clones in culture

Biochemistry ◽  
1980 ◽  
Vol 19 (1) ◽  
pp. 169-176 ◽  
Author(s):  
Ryuichiro Hata ◽  
Yoshifumi Ninomiya ◽  
Yutaka Nagai ◽  
Yutaka Tsukada
Keyword(s):  
Rat Liver ◽  
Parenchymal Cell ◽  
Liver Parenchymal Cell ◽  
Liver Parenchymal

l-Thyroxine enters the rat liver cell by simple diffusion

1983 ◽  
Vol 97 (2) ◽  
pp. 277-282 ◽  
Author(s):  
G. S. Rao ◽  
M. L. Rao
Keyword(s):  
Rat Liver ◽  
Arrhenius Plot ◽  
Parenchymal Cell ◽  
Liver Cells ◽  
Liver Parenchymal Cell ◽  
Simple Diffusion ◽  
Liver Parenchymal ◽  
Isolated Rat Liver ◽  
Centrifugation Technique ◽  
Parenchymal Cells

The mode of uptake of l-[125I]thyroxine by freshly isolated rat liver parenchymal cells was studied by a rapid centrifugation technique. Using conditions for measuring initial rates of uptake, uptake by liver cells was not saturable when exposed to hormone concentrations in the incubation medium ranging from 2 pmol/l to 10 μmol/l. The Arrhenius plot was linear from 2 to 37°C; the temperature coefficient was 1·4. The uptake of l-[125I]thyroxine by liver cells was 35% when compared with that of l-[125I]tri-iodothyronine. In the presence of 2·8% bovine serum albumin the rate of uptake of l-[125I]thyroxine by liver cells was reduced by 90%. These results suggest that l-[125I]thyroxine enters the rat liver parenchymal cell by simple diffusion and only the free hormone crosses the plasma membrane.

scholarly journals LIVER PARENCHYMAL CELL INJURY

1965 ◽  
Vol 25 (3) ◽  
pp. 53-75 ◽  
Author(s):  
Edward S. Reynolds
Keyword(s):  
Rat Liver ◽  
Inorganic Phosphate ◽  
Cell Injury ◽  
Parenchymal Cell ◽  
Mitochondrial Atpase ◽  
Liver Parenchymal Cell ◽  
Liver Parenchymal ◽  
Serum Inorganic Phosphate ◽  
Chemical Events ◽  
Parenchymal Cells

Accumulation of calcium in the mitochondria of rat liver parenchymal cells at 16 and 24 hours after poisoning with carbon tetrachloride is associated with an increase in amount of liver inorganic phosphate, the persistence of mitochondrial adenosine triphosphatase activity, and the formation of electron-opaque intramitochondrial masses in cells with increased calcium contents. These masses, which form within the mitochondrial matrix adjacent to internal mitochondrial membranes, resemble those observed in isolated mitochondria which accumulate calcium and inorganic phosphate; are present in a locus similar to that of electron opacities which result from electron-histochemical determination of mitochondrial ATPase activity; and differ in both appearance and position from matrix granules of normal mitochondria. After poisoning, normal matrix granules disappear from mitochondria prior to their accumulation of calcium. As calcium-associated electron-opaque intramitochondrial masses increase in size, mitochondria degenerate in appearance. At the same time, cytoplasmic membrane systems of mid-zonal and centrilobular cells are disrupted by degranulation of the rough endoplasmic reticulum and the formation of labyrinthine tubular aggregates. The increase in amount of inorganic phosphate in rat liver following poisoning is balanced by a decreased amount of phosphoprotein. These chemical events do not appear to be related, however, as the inorganic phosphate accumulated is derived from serum inorganic phosphate.

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