Kinetics of acyl-tRNA complexes of Escherichia coli phenylalanyl-tRNA synthetase. A conformational change is rate limiting in catalysis

Biochemistry ◽  
1982 ◽  
Vol 21 (10) ◽  
pp. 2460-2467 ◽  
Author(s):  
Mireille Baltzinger ◽  
Eggehard Holler
Keyword(s):  
Escherichia Coli ◽  
Conformational Change ◽  
Trna Synthetase ◽  
Rate Limiting ◽  
Kinetics Of

scholarly journals Escherichia coli Peptidase A, B, or N Can Process Translation Inhibitor Microcin C

2008 ◽  
Vol 190 (7) ◽  
pp. 2607-2610 ◽  
Author(s):  
Teymur Kazakov ◽  
Gaston H. Vondenhoff ◽  
Kirill A. Datsenko ◽  
Maria Novikova ◽  
Anastasia Metlitskaya ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Growth ◽  
Trna Synthetase ◽  
Methionine Residue ◽  
Rate Limiting Step ◽  
Limiting Step ◽  
Translation Inhibitor ◽  
Rate Limiting ◽  
Broad Specificity

ABSTRACT The heptapeptide-nucleotide microcin C (McC) targets aspartyl-tRNA synthetase. Upon its entry into a susceptible cell, McC is processed to release a nonhydrolyzable aspartyl-adenylate that inhibits aspartyl-tRNA synthetase, leading to the cessation of translation and cell growth. Here, we surveyed Escherichia coli cells with singly, doubly, and triply disrupted broad-specificity peptidase genes to show that any of three nonspecific oligopeptidases (PepA, PepB, or PepN) can effectively process McC. We also show that the rate-limiting step of McC processing in vitro is deformylation of the first methionine residue of McC.

scholarly journals Chorismate synthase. Pre-steady-state kinetics of phosphate release from 5-enolpyruvylshikimate 3-phosphate

1990 ◽  
Vol 265 (3) ◽  
pp. 899-902 ◽  
Author(s):  
T R Hawkes ◽  
T Lewis ◽  
J R Coggins ◽  
D M Mousdale ◽  
D J Lowe ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Steady State ◽  
Lag Phase ◽  
Chorismate Synthase ◽  
Phosphate Release ◽  
Rate Limiting Step ◽  
Limiting Step ◽  
Steady State Kinetics ◽  
Rate Limiting ◽  
Kinetics Of

The pre-steady-state kinetics of phosphate formation from 5-enolpyruvylshikimate 3-phosphate catalysed by Escherichia coli chorismate synthase (EC 4.6.1.4) were studied by a rapid-acid-quench technique at 25 degrees C at pH 7.5. No pre-steady-state ‘burst’ or ‘lag’ phase was observed, showing that phosphate is released concomitant with the rate-limiting step of the enzyme. The implications of this result for the mechanism of action of chorismate synthase are discussed.

scholarly journals A Novel Initiation Pathway in Escherichia Coli Transcription

2016 ◽  
Author(s):  
Eitan Lerner ◽  
SangYoon Chung ◽  
Benjamin L. Allen ◽  
Shuang Wang ◽  
Jookyung J. Lee ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Transcription Initiation ◽  
Rate Limiting Step ◽  
Limiting Step ◽  
Magnetic Tweezer ◽  
Delayed Initiation ◽  
Rate Limiting ◽  
Kinetics Of

AbstractInitiation is a highly regulated, rate-limiting step in transcription. We employed a series of approaches to examine the kinetics of RNA polymerase (RNAP) transcription initiation in greater detail. Quenched kinetics assays, in combination with magnetic tweezer experiments and other methods, showed that, contrary to expectations, RNAP exit kinetics from later stages of initiation (e.g. from a 7-base transcript) was markedly slower than from earlier stages. Further examination implicated a previously unidentified intermediate in which RNAP adopted a long-lived backtracked state during initiation. In agreement, the RNAP-GreA endonuclease accelerated transcription kinetics from otherwise delayed initiation states and prevented RNAP backtracking. Our results indicate a previously uncharacterized RNAP initiation state that could be exploited for therapeutic purposes and may reflect a conserved intermediate among paused, initiating eukaryotic enzymes.Significance:Transcription initiation by RNAP is rate limiting owing to many factors, including a newly discovered slow initiation pathway characterized by RNA backtracking and pausing. This backtracked and paused state occurs when all NTPs are present in equal amounts, but becomes more prevalent with NTP shortage, which mimics cellular stress conditions. Pausing and backtracking in initiation may play an important role in transcriptional regulation, and similar backtracked states may contribute to pausing among eukaryotic RNA polymerase II enzymes.

Phenylalanyl-tRNA synthetase induced conformational change of Escherichia coli tRNAPhe

Biochemistry ◽  
1979 ◽  
Vol 18 (13) ◽  
pp. 2887-2895 ◽  
Author(s):  
A. Favre ◽  
J. P. Ballini ◽  
E. Holler
Keyword(s):  
Escherichia Coli ◽  
Conformational Change ◽  
Trna Synthetase

scholarly journals Rapid kinetics of G protein subunit association: A rate-limiting conformational change?

FEBS Letters ◽  
1994 ◽  
Vol 355 (3) ◽  
pp. 251-253 ◽  
Author(s):  
Richard R. Neubig ◽  
Mark P. Connolly ◽  
Ann E. Remmers
Keyword(s):  
Conformational Change ◽  
G Protein ◽  
Protein Subunit ◽  
Rapid Kinetics ◽  
Rate Limiting ◽  
Kinetics Of

scholarly journals In vivo kinetics of transcription initiation of the lar promoter in Escherichia coli. Evidence for a sequential mechanism with two rate-limiting steps

2011 ◽  
Vol 5 (1) ◽  
pp. 149 ◽  
Author(s):  
Meenakshisundaram Kandhavelu ◽  
Henrik Mannerström ◽  
Abhishekh Gupta ◽  
Antti Häkkinen ◽  
Jason Lloyd-Price ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Transcription Initiation ◽  
In Vivo Kinetics ◽  
Sequential Mechanism ◽  
Rate Limiting ◽  
Kinetics Of
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