Kinetics of hepatic cytochrome P-450 reduction: correlation with spin state of the ferric heme

Biochemistry ◽  
1982 ◽  
Vol 21 (6) ◽  
pp. 1324-1330 ◽  
Author(s):  
Wayne L. Backes ◽  
Stephen G. Sligar ◽  
John B. Schenkman
Keyword(s):  
Spin State ◽  
Ferric Heme ◽  
Kinetics Of ◽  
Cytochrome P 450 ◽  
Hepatic Cytochrome

Reduction kinetics of purified rat liver cytochrome P-450. Evidence for a sequential reaction mechanism dependent on the hemoprotein spin state

Biochemistry ◽  
1984 ◽  
Vol 23 (20) ◽  
pp. 4526-4533 ◽  
Author(s):  
Paul P. Tamburini ◽  
G. Gordon Gibson ◽  
Wayne L. Backes ◽  
Stephen G. Sligar ◽  
John B. Schenkman
Keyword(s):  
Reaction Mechanism ◽  
Rat Liver ◽  
Spin State ◽  
Reduction Kinetics ◽  
Sequential Reaction ◽  
Liver Cytochrome ◽  
Kinetics Of ◽  
Cytochrome P 450

The Nature of Hepatic Cytochrome P-450 Induced in Hexachlorobenzene-Fed Rats

1978 ◽  
Vol 55 (5) ◽  
pp. 461-469
Author(s):  
G. H. Blekkenhorst ◽  
L. Eales ◽  
N. R. Pimstone
Keyword(s):  
Catalytic Properties ◽  
Enzyme System ◽  
Spectral Studies ◽  
Time Intervals ◽  
Female Wistar Rats ◽  
Kinetics Of ◽  
Cytochrome P 450 ◽  
Effect Of Feeding ◽  
Hepatic Cytochrome

1. The effect of feeding with a diet containing 0.2% (w/w) hexachlorobenzene on hepatic and urinary porphyrins and hepatic cytochrome P-450 was studied at various time intervals in female Wistar rats. 2. Hexachlorobenzene administration for 45 days resulted in the development of porphyria in rats, which biochemically closely resembles symptomatic porphyria in humans, with elevation of urinary uroporphyrin excretion, hepatic uroporphyrin content, and hepatic cytochrome P-450 content, in addition to appearance of porphyrins of the isocoproporphyrin (P1) series in the faeces. 3. Spectral studies of the induced hepatic cytochrome P-450 at 45 days with carbon monoxide and ethyl isocyanide as ligands indicated the presence of a greater admixture of a haemoprotein distinct from cytochrome P-450. 4. Study in vitro of the kinetics of two reactions, namely aminopyrine N-demethylation and 3,4-benzpyrene hydroxylation, catalysed by the hepatic microsomal cytochrome P-450-dependent enzyme system, suggested that hexachlorobenzene induced a form of cytochrome P-450 with different catalytic properties from those of forms induced by either phenobarbital or 3-methylcholanthrene.

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