Strand-scission of DNA by neocarzinostatin, auromomycin, and bleomycin: studies on base release and nucleotide sequence specificity

Biochemistry ◽  
1981 ◽  
Vol 20 (26) ◽  
pp. 7599-7606 ◽  
Author(s):  
Masaru Takeshita ◽  
Lizzy S. Kappen ◽  
Arthur P. Grollman ◽  
Moises Eisenberg ◽  
Irving H. Goldberg
Keyword(s):  
Nucleotide Sequence ◽  
Sequence Specificity ◽  
Strand Scission

scholarly journals Nucleotide Sequence Specificity in DNA Cleavage Caused by Dihydropyrazines, a New Type of DNA Strand-Cleaving Agent.

2000 ◽  
Vol 23 (11) ◽  
pp. 1281-1286 ◽  
Author(s):  
Nobuhiro KASHIGE ◽  
Tadatoshi YAMAGUCHI ◽  
Fumio MIAKE ◽  
Kenji WATANABE
Keyword(s):  
Nucleotide Sequence ◽  
Dna Cleavage ◽  
Sequence Specificity ◽  
New Type ◽  
Dna Strand

scholarly journals First Evidence of Bud Feeding-Induced RNAi in a Crop Pest via Exogenous Application of dsRNA

Insects ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 769
Author(s):  
Jonathan Willow ◽  
Liina Soonvald ◽  
Silva Sulg ◽  
Riina Kaasik ◽  
Ana Isabel Silva ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Gene Silencing ◽  
Oilseed Rape ◽  
Dietary Exposure ◽  
Exposure Period ◽  
Sequence Specificity ◽  
Pollen Beetle ◽  
Brassicogethes Aeneus ◽  
Model Control ◽  
Potential Strategy

Spray-induced gene silencing (SIGS) is a potential strategy for agricultural pest management, whereby nucleotide sequence-specific double-stranded RNA (dsRNA) can be sprayed onto a crop; the desired effect being a consumption of dsRNA by the target pest, and subsequent gene silencing-induced mortality. Nucleotide sequence-specificity is the basis for dsRNA’s perceived biosafety. A biosafe approach to pollen beetle (Brassicogethes aeneus) management in oilseed rape (Brassica napus) agroecosystems is needed. We examined the potential for SIGS in B. aeneus, via bud feeding, a field-relevant dsRNA exposure route. Oilseed rape buds were uniformly treated with dsRNA designed to target αCOP in B. aeneus. Our model control dsRNA (dsGFP) remained detectable on buds throughout the entire 3 d exposure period. When applied at 5 µg/µL, dsαCOP induced significant αCOP silencing 3 d after dietary exposure to buds treated with this dsαCOP concentration. We also observed a trend of increased αCOP silencing with increasing concentrations of dsαCOP at both 3 and 6 d. Furthermore, we observed a marginally significant and significant reduction in B. aeneus survival at 10 and 15 d, respectively. Our results suggest potential for developing a SIGS approach to B. aeneus management—though further experiments are needed to more fully understand this potential.

Human cytokine gene nucleotide sequence alignments, 1998

1998 ◽  
Vol 25 (2‐3) ◽  
pp. 83-265 ◽  
Author(s):  
J. L. Bidwell ◽  
N. A. P. Wood ◽  
H. R. Morse ◽  
O. O. Olomolaiye ◽  
G. J. Laundy
Keyword(s):  
Nucleotide Sequence ◽  
Cytokine Gene ◽  
Sequence Alignments ◽  
Gene Nucleotide Sequence ◽  
Human Cytokine
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