Synthesis and assembly of cytochrome c oxidase in synchronous cultures of yeast

Biochemistry ◽  
1981 ◽  
Vol 20 (19) ◽  
pp. 5369-5373 ◽  
Author(s):  
T. Somasundaram ◽  
J. Jayaraman
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Synchronous Cultures

scholarly journals Changes in respiratory activities during the cell-cycle of the fission yeast Schizosaccharomyces pombe 972h- growing in the presence of glycerol

1974 ◽  
Vol 144 (1) ◽  
pp. 141-148 ◽  
Author(s):  
Robert K. Poole ◽  
David Lloyd
Keyword(s):  
Cell Cycle ◽  
Cytochrome C ◽  
Schizosaccharomyces Pombe ◽  
Succinate Dehydrogenase ◽  
Cytochrome C Oxidase ◽  
Similar Time ◽  
Batch Cultures ◽  
Synchronous Cultures ◽  
Time Courses ◽  
Nadh Cytochrome

1. The specific activities of cytochrome c oxidase, catalase, succinate dehydrogenase, succinate–cytochrome c oxidoreductase, NADH–cytochrome c oxidoreductase, and NADPH–cytochrome c oxidoreductase in mid-exponential-phase batch cultures of glycerol-grown Schizosaccharomyces pombe indicated that the organisms were catabolite-de-repressed. 2. In cultures growing synchronously in the presence of glycerol as sole carbon source, the respiration rate showed two abrupt increases at about 0.45 and 0.95 of the cell-cycle and remained constant in the periods between successive rises. 3. Catalase, succinate dehydrogenase, NADH–cytochrome c oxidoreductase and acid p-nitrophenyl-phosphatase all showed peak patterns of expression in synchronous cultures. 4. Cytochrome c oxidase and cytochromes a+a3 both showed step patterns of expression with two rises per cell-cycle. 5. Cytochromes c548, b554 and b560 all followed similar time-courses in step patterns of expression, but these were distinct from, and more complex than, that of cytochromes a+a3. 6. These results are compared with those previously obtained with glucose-grown cultures, and the part played by catabolite repression in the expression of respiratory activities in the cell-cycle is assessed.

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