A method for isolation of oligo(uridylic acid)-containing messenger ribonucleic acid from Hela cells

Biochemistry ◽  
1981 ◽  
Vol 20 (19) ◽  
pp. 5359-5364 ◽  
Author(s):  
William M. Wood ◽  
Mary Edmonds
Keyword(s):  
Hela Cells ◽  
Ribonucleic Acid ◽  
Messenger Ribonucleic Acid

Location of oligo(uridylic acid) sequences within messenger ribonucleic acid molecules of HeLa cells

Biochemistry ◽  
1985 ◽  
Vol 24 (14) ◽  
pp. 3678-3686 ◽  
Author(s):  
Joseph W. Kulkosky ◽  
William M. Wood ◽  
Mary Edmonds
Keyword(s):  
Hela Cells ◽  
Ribonucleic Acid ◽  
Messenger Ribonucleic Acid

scholarly journals Synthesis and turnover of mitochondrial ribonucleic acid in HeLa cells: the mature ribosomal and messenger ribonucleic acid species are metabolically unstable.

1981 ◽  
Vol 1 (6) ◽  
pp. 497-511 ◽  
Author(s):  
R Gelfand ◽  
G Attardi
Keyword(s):  
Ribosomal Rna ◽  
Hela Cells ◽  
Ribonucleic Acid ◽  
Half Life ◽  
Messenger Rna ◽  
Specific Activity ◽  
Mitochondrial Rna ◽  
Messenger Rnas ◽  
Precursor Pool ◽  
Messenger Ribonucleic Acid

The synthesis rates and half-lives of the individual mitochondrial ribosomal ribonucleic acid (RNA) and polyadenylic acid-containing RNA species in HeLa cells have been determined by analyzing their kinetics of labeling with [5-3H]-uridine and the changes in specific activity of the mitochondrial nucleotide precursor pools. In one experiment, a novel method for determining the nucleotide precursor pool specific activities, using nascent RNA chains, has been utilized. All mitochondrial RNA species analyzed were found to be metabolically unstable, with half-lives of 2.5 to 3.5 h for the two ribosomal RNA components and between 25 and 90 min for the various putative messenger RNAs. A cordycepin "chase" experiment yielded half-life values for the messenger RNA species which were, in general, larger by a factor of 1.5 to 2.5 than those estimated in the labeling kinetics experiments. On the basis of previous observations, a model is proposed whereby the rate of mitochondrial RNA decay is under feedback control by some mechanism linked to RNA synthesis or processing. A short half-life was determined for five large polyadenylated RNAs, which are probably precursors of mature species. A rate of synthesis of one to two molecules per minute per cell was estimated for the various H-strand-coded messenger RNA species, and a rate of synthesis 50 to 100 times higher was estimated for the ribosomal RNA species. These data indicate that the major portion of the H-strand in each mitochondrial deoxyribonucleic acid molecule is transcribed very infrequently, possibly as rarely as once or twice per cell generation. Furthermore, these results are consistent with a previously proposed model of H-strand transcription in the form of a single polycistronic molecule.

Poly(uridylic acid) sequences in messenger ribonucleic acid of HeLa cells

Biochemistry ◽  
1976 ◽  
Vol 15 (21) ◽  
pp. 4643-4649 ◽  
Author(s):  
Edward L. Korwek ◽  
Hiroshi Nakazato ◽  
S. Venkatesan ◽  
Mary Edmonds
Keyword(s):  
Hela Cells ◽  
Ribonucleic Acid ◽  
Messenger Ribonucleic Acid

scholarly journals Synthesis and turnover of mitochondrial ribonucleic acid in HeLa cells: the mature ribosomal and messenger ribonucleic acid species are metabolically unstable

1981 ◽  
Vol 1 (6) ◽  
pp. 497-511
Author(s):  
R Gelfand ◽  
G Attardi
Keyword(s):  
Ribosomal Rna ◽  
Hela Cells ◽  
Ribonucleic Acid ◽  
Half Life ◽  
Messenger Rna ◽  
Specific Activity ◽  
Mitochondrial Rna ◽  
Messenger Rnas ◽  
Precursor Pool ◽  
Messenger Ribonucleic Acid

The synthesis rates and half-lives of the individual mitochondrial ribosomal ribonucleic acid (RNA) and polyadenylic acid-containing RNA species in HeLa cells have been determined by analyzing their kinetics of labeling with [5-3H]-uridine and the changes in specific activity of the mitochondrial nucleotide precursor pools. In one experiment, a novel method for determining the nucleotide precursor pool specific activities, using nascent RNA chains, has been utilized. All mitochondrial RNA species analyzed were found to be metabolically unstable, with half-lives of 2.5 to 3.5 h for the two ribosomal RNA components and between 25 and 90 min for the various putative messenger RNAs. A cordycepin "chase" experiment yielded half-life values for the messenger RNA species which were, in general, larger by a factor of 1.5 to 2.5 than those estimated in the labeling kinetics experiments. On the basis of previous observations, a model is proposed whereby the rate of mitochondrial RNA decay is under feedback control by some mechanism linked to RNA synthesis or processing. A short half-life was determined for five large polyadenylated RNAs, which are probably precursors of mature species. A rate of synthesis of one to two molecules per minute per cell was estimated for the various H-strand-coded messenger RNA species, and a rate of synthesis 50 to 100 times higher was estimated for the ribosomal RNA species. These data indicate that the major portion of the H-strand in each mitochondrial deoxyribonucleic acid molecule is transcribed very infrequently, possibly as rarely as once or twice per cell generation. Furthermore, these results are consistent with a previously proposed model of H-strand transcription in the form of a single polycistronic molecule.

Sequence content of oligo(uridylic acid)-containing messenger ribonucleic acid from HeLa cells

Biochemistry ◽  
1985 ◽  
Vol 24 (14) ◽  
pp. 3686-3693 ◽  
Author(s):  
William M. Wood ◽  
John C. Wallace ◽  
Mary Edmonds
Keyword(s):  
Hela Cells ◽  
Ribonucleic Acid ◽  
Messenger Ribonucleic Acid
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