Hazards and their exploitation in the applications of molecular biology to structure-function relationships

Biochemistry ◽  
1990 ◽  
Vol 29 (41) ◽  
pp. 9495-9502 ◽  
Author(s):  
Paul Schimmel
Keyword(s):  
Molecular Biology ◽  
Structure Function

Molecular biology of the human apolipoprotein genes: gene regulation and structure/function relationship

1992 ◽  
Vol 3 (2) ◽  
pp. 96-113 ◽  
Author(s):  
Vassilis I. Zannis ◽  
Dimitris Kardassis ◽  
Philippe Cardot ◽  
Margarita Hadzopoulou-Cladaras ◽  
Eleni E. Zanni ◽  
...  
Keyword(s):  
Gene Regulation ◽  
Molecular Biology ◽  
Structure Function ◽  
Human Apolipoprotein ◽  
Structure Function Relationship ◽  
Function Relationship

scholarly journals The whole is greater than the sum of its parts: at the intersection of order, disorder, and kinetochore function

2020 ◽  
Vol 64 (2) ◽  
pp. 349-358
Author(s):  
Margaux R. Audett ◽  
Thomas J. Maresca
Keyword(s):  
Molecular Biology ◽  
Structure Function ◽  
Spatial Organization ◽  
Disordered Proteins ◽  
Intrinsically Disordered ◽  
The Past ◽  
Massive Structure ◽  
In Vitro Reconstitution ◽  
Kinetochore Function

Abstract The kinetochore (KT) field has matured tremendously since Earnshaw first identified CENP-A, CENP-B, and CENP-C [1,2]. In the past 35 years, the accumulation of knowledge has included: defining the parts list, identifying epistatic networks of interdependence within the parts list, understanding the spatial organization of subcomplexes into a massive structure – hundreds of megadaltons in size, and dissecting the functions of the KT in its entirety as well as of its individual parts. Like nearly all cell and molecular biology fields, the structure–function paradigm has been foundational to advances in the KT field. A point nicely highlighted by the fact that we are at the precipice of the in vitro reconstitution of a functional KT holo complex. Yet conventional notions of structure cannot provide a complete picture of the KT especially since it contains an abundance of unstructured or intrinsically disordered constituents. The combination of structured and disordered proteins within the KT results in an assembled system that is functionally greater than the sum of its parts.

scholarly journals Combining a Simple Method for DNA/RNA/Protein Co-Purification and Arabidopsis Protoplast Assay to Facilitate Viroid Research

Viruses ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 324 ◽  
Author(s):  
Jian Jiang ◽  
Junfei Ma ◽  
Bin Liu ◽  
Ying Wang
Keyword(s):  
Molecular Biology ◽  
Structure Function ◽  
High Throughput ◽  
Noncoding Rnas ◽  
Protein Precipitation ◽  
New Method ◽  
Robust Method ◽  
Simple Method ◽  
Functional Studies ◽  
Arabidopsis Protoplast

Plant–viroid interactions represent a valuable model for delineating structure–function relationships of noncoding RNAs. For various functional studies, it is desirable to minimize sample variations by using DNA, RNA, and proteins co-purified from the same samples. Currently, most of the co-purification protocols rely on TRI Reagent (Trizol as a common representative) and require protein precipitation and dissolving steps, which render difficulties in experimental handling and high-throughput analyses. Here, we established a simple and robust method to minimize the precipitation steps and yield ready-to-use RNA and protein in solutions. This method can be applied to samples in small quantities, such as protoplasts. Given the ease and the robustness of this new method, it will have broad applications in virology and other disciplines in molecular biology.

Plant Lipid-Transfer Proteins: Structure, Function and Molecular Biology

1989 ◽  
pp. 341-350 ◽  
Author(s):  
V. Arondel ◽  
F. Tchang ◽  
Ch. Vergnolle ◽  
A. Jolliot ◽  
M. Grosbois ◽  
...  
Keyword(s):  
Molecular Biology ◽  
Structure Function ◽  
Lipid Transfer ◽  
Lipid Transfer Proteins ◽  
Plant Lipid
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