Structurally and catalytically important residues in the phosphate binding loop of adenylate kinase of Escherichia coli

Biochemistry ◽  
1990 ◽  
Vol 29 (32) ◽  
pp. 7451-7459 ◽  
Author(s):  
Jochen Reinstein ◽  
Ilme Schlichting ◽  
Alfred Wittinghofer
Keyword(s):  
Escherichia Coli ◽  
Adenylate Kinase ◽  
Phosphate Binding ◽  
Binding Loop

scholarly journals Cooperative and Critical Roles for Both G Domains in the GTPase Activity and Cellular Function of Ribosome-Associated Escherichia coli EngA

2006 ◽  
Vol 188 (22) ◽  
pp. 7992-7996 ◽  
Author(s):  
Amrita Bharat ◽  
Mengxi Jiang ◽  
Susan M. Sullivan ◽  
Janine R. Maddock ◽  
Eric D. Brown
Keyword(s):  
Escherichia Coli ◽  
Ribosomal Subunit ◽  
Cellular Function ◽  
Gtpase Activity ◽  
Cellular Phenotype ◽  
Phosphate Binding ◽  
Biochemical Function ◽  
Polysome Profile ◽  
Binding Loop

ABSTRACT To probe the cellular phenotype and biochemical function associated with the G domains of Escherichia coli EngA (YfgK, Der), mutations were created in the phosphate binding loop of each. Neither an S16A nor an S217A variant of G domain 1 or 2, respectively, was able to support growth of an engA conditional null. Polysome profiles of EngA-depleted cells were significantly altered, and His6-EngA was found to cofractionate with the 50S ribosomal subunit. The variants were unable to complement the abnormal polysome profile and were furthermore significantly impacted with respect to in vitro GTPase activity. Together, these observations suggest that the G domains have a cooperative function in ribosome stability and/or biogenesis.

Vanadate catalyzes photocleavage of adenylate kinase at proline-17 in the phosphate-binding loop

Biochemistry ◽  
1992 ◽  
Vol 31 (2) ◽  
pp. 491-497 ◽  
Author(s):  
Christine R. Cremo ◽  
Joseph A. Loo ◽  
Charles G. Edmonds ◽  
Kristina M. Hatlelid
Keyword(s):  
Adenylate Kinase ◽  
Phosphate Binding ◽  
Binding Loop

Mutations in the nucleotide binding loop of adenylate kinase of Escherichia coli

Biochemistry ◽  
1988 ◽  
Vol 27 (13) ◽  
pp. 4712-4720 ◽  
Author(s):  
Jochen Reinstein ◽  
Martin Brune ◽  
Alfred Wittinghofer
Keyword(s):  
Escherichia Coli ◽  
Adenylate Kinase ◽  
Nucleotide Binding ◽  
Binding Loop

scholarly journals Circular dichroism investigation of Escherichia coli adenylate kinase.

1987 ◽  
Vol 262 (6) ◽  
pp. 2502-2506
Author(s):  
M. Monnot ◽  
A.M. Gilles ◽  
I.S. Girons ◽  
S. Michelson ◽  
O. Bârzu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Circular Dichroism ◽  
Adenylate Kinase ◽  
Circular Dichroïsm

scholarly journals Structural and catalytic properties of a deletion derivative (delta 133-157) of Escherichia coli adenylate kinase

1991 ◽  
Vol 266 (17) ◽  
pp. 10781-10786
Author(s):  
T. Rose ◽  
M. Brune ◽  
A. Wittinghofer ◽  
K. Le Blay ◽  
W.K. Surewicz ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Adenylate Kinase ◽  
Catalytic Properties ◽  
Deletion Derivative
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