Evaluation of membrane phase behavior as a tool to detect extrinsic protein-induced domain formation: binding of prothrombin to phosphatidylserine/phosphatidylcholine vesicles

Biochemistry ◽  
1990 ◽  
Vol 29 (28) ◽  
pp. 6720-6729 ◽  
Author(s):  
Susan W. Tendian ◽  
Barry R. Lentz
Keyword(s):  
Phase Behavior ◽  
Domain Formation ◽  
Membrane Phase

Cryopreservation of platelets using trehalose: The role of membrane phase behavior during freezing

2012 ◽  
Vol 28 (5) ◽  
pp. 1347-1354 ◽  
Author(s):  
Christiane Gläfke ◽  
Maryam Akhoondi ◽  
Harriëtte Oldenhof ◽  
Harald Sieme ◽  
Willem F. Wolkers
Keyword(s):  
Phase Behavior ◽  
Membrane Phase

Osmotic properties and membrane phase behavior during cooling of stallion sperm

Cryobiology ◽  
2011 ◽  
Vol 63 (3) ◽  
pp. 310-311
Author(s):  
Harriëtte Oldenhof ◽  
Marina Gojowsky ◽  
Willem F Wolkers ◽  
Harald Sieme
Keyword(s):  
Phase Behavior ◽  
Membrane Phase ◽  
Osmotic Properties

37. Freeze Injury in mammalian cells predicted by membrane phase behavior

Cryobiology ◽  
2007 ◽  
Vol 55 (3) ◽  
pp. 336
Author(s):  
Saravana Kumar Balasubramanian ◽  
Kristen Bartelt ◽  
Willem Wolkers ◽  
John Bischof
Keyword(s):  
Phase Behavior ◽  
Mammalian Cells ◽  
Membrane Phase ◽  
Freeze Injury

Effect of Me2SO on Membrane Phase Behavior and Protein Denaturation of Human Pulmonary Endothelial Cells Studied by In Situ FTIR Spectroscopy

2009 ◽  
Vol 131 (7) ◽  
Author(s):  
Ralf Spindler ◽  
Willem F. Wolkers ◽  
Birgit Glasmacher
Keyword(s):  
Endothelial Cells ◽  
Phase Behavior ◽  
Wide Temperature Range ◽  
Protein Denaturation ◽  
Onset Temperature ◽  
Cellular Proteins ◽  
Membrane Phase ◽  
Conformational Disorder ◽  
Temperature Range ◽  
Pulmonary Endothelial Cells

Fourier transform infrared spectroscopy (FTIR) provides a unique technique to study membranes and proteins within their native cellular environment. FTIR was used here to study the effects of dimethyl sulfoxide (Me2SO) on membranes and proteins in human pulmonary endothelial cells (HPMECs). Temperature-dependent changes in characteristic lipid and protein vibrational bands were identified to reveal the effects of Me2SO on membrane phase behavior and protein stability. At Me2SO concentrations equal to or below 10% (v/v), Me2SO was found to decrease membrane conformational disorder. At higher Me2SO concentrations (15% v/v), however, membrane conformational disorder was found to be similar to that of cells in the absence of Me2SO. This effect was observed over a wide temperature range from 90°C down to −40°C. Me2SO had no clear effects on cellular proteins during freezing. During heating, however, Me2SO had a destabilizing effect on cellular proteins. In the absence of Me2SO, protein denaturation started at an onset temperature of 46°C, whereas at 15% Me2SO the onset temperature of protein denaturation decreased to 32°C. This implies that in the presence of Me2SO the onset temperature of protein denaturation is lower than the normal growth temperature of the cells, which could explain the well documented toxic effect of Me2SO at physiological temperatures. Me2SO destabilizes cellular proteins during heating and decreases membrane conformational disorder over a wide temperature range.

Membrane phase behavior of a psychrotrophic and mesophilic bacillus

1979 ◽  
Vol 2 (3) ◽  
pp. 147-150 ◽  
Author(s):  
W. E. Inniss ◽  
J. E. Thompson ◽  
C. I. Mayfield
Keyword(s):  
Phase Behavior ◽  
Membrane Phase

Cooperative effects of fatty acids and n-butanol on lipid membrane phase behavior

2016 ◽  
Vol 139 ◽  
pp. 62-67 ◽  
Author(s):  
Geoffrey D. Bothun ◽  
Lauren Boltz ◽  
Yogi Kurniawan ◽  
Carmen Scholz
Keyword(s):  
Fatty Acids ◽  
Phase Behavior ◽  
Lipid Membrane ◽  
Membrane Phase ◽  
Cooperative Effects

Membrane phase behavior during cooling of stallion sperm and its correlation with freezability

2012 ◽  
Vol 29 (3-4) ◽  
pp. 95-106 ◽  
Author(s):  
Harriëtte Oldenhof ◽  
Katharina Friedel ◽  
Maryam Akhoondi ◽  
Marina Gojowsky ◽  
Willem F. Wolkers ◽  
...  
Keyword(s):  
Phase Behavior ◽  
Membrane Phase

Effect of a Fullerene Water Suspension on Bacterial Phospholipids and Membrane Phase Behavior

2007 ◽  
Vol 41 (7) ◽  
pp. 2636-2642 ◽  
Author(s):  
Jiasong Fang ◽  
Delina Y. Lyon ◽  
Mark R. Wiesner ◽  
Jinping Dong ◽  
Alvarez
Keyword(s):  
Phase Behavior ◽  
Membrane Phase ◽  
Water Suspension

scholarly journals High Content Imaging to Identify Modulators of Membrane Phase Behavior

2020 ◽  
Vol 118 (3) ◽  
pp. 77a
Author(s):  
Nico Fricke ◽  
Ajit Tiwari ◽  
Krishnan Raghunathan ◽  
Hui Huang ◽  
Ricardo F. Capone ◽  
...  
Keyword(s):  
Phase Behavior ◽  
Membrane Phase ◽  
High Content Imaging
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