Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase: physicochemical characteristics of the nucleotide binding site, as deduced from fluorescent spectroscopy measurements

Biochemistry ◽  
1990 ◽  
Vol 29 (19) ◽  
pp. 4548-4553 ◽  
Author(s):  
M. Victoria Encinas ◽  
Veronica Quinones ◽  
Emilio Cardemil
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Binding Site ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Nucleotide Binding ◽  
Physicochemical Characteristics ◽  
Nucleotide Binding Site ◽  
Fluorescent Spectroscopy

Motor proteins in Saccharomyces cerevisiae

1995 ◽  
Vol 73 (S1) ◽  
pp. 369-371 ◽  
Author(s):  
Susan S. Brown
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Binding Site ◽  
Key Words ◽  
Motor Proteins ◽  
Nucleotide Binding ◽  
Nucleotide Binding Site ◽  
Temperature Sensitive ◽  
Related Protein ◽  
Yeast Saccharomyces Cerevisiae

A number of myosins have been identified in yeast (Saccharomyces cerevisiae), an organism ideally suited to dissecting out their different functions. We have learned that a temperature-sensitive defect in one of these myosins (Myo2p) can be partially overcome by overexpression of a kinesin-like protein (Smy1p). This raises the possibility of the involvement of microtubules in the same function as Myo2p. However, we have been unable to demonstrate that this is the case, either using nocodazole to depolymerize microtubules or by altering the nucleotide-binding site of Smy1p. Key words: myosin, kinesin-related protein, cytoskeleton.

Characterization of Nucleotide Binding ­Site-Encoding Genes in Sweetpotato, Ipomoea batatas(L.) Lam., and Their Response to Biotic and Abiotic Stresses

2021 ◽  
pp. 1-15
Author(s):  
Zengzhi Si ◽  
Yake Qiao ◽  
Kai Zhang ◽  
Zhixin Ji ◽  
Jinling Han
Keyword(s):  
Binding Site ◽  
Abiotic Stresses ◽  
Ipomoea Batatas ◽  
Expression Profiles ◽  
Nucleotide Binding ◽  
Regulatory Elements ◽  
Nucleotide Binding Site ◽  
Biotic And Abiotic Stresses ◽  
Encoding Genes

Sweetpotato, <i>Ipomoea batatas</i> (L.) Lam., is an important and widely grown crop, yet its production is affected severely by biotic and abiotic stresses. The nucleotide binding site (NBS)-encoding genes have been shown to improve stress tolerance in several plant species. However, the characterization of NBS-encoding genes in sweetpotato is not well-documented to date. In this study, a comprehensive analysis of NBS-encoding genes has been conducted on this species by using bioinformatics and molecular biology methods. A total of 315 NBS-encoding genes were identified, and 260 of them contained all essential conserved domains while 55 genes were truncated. Based on domain architectures, the 260 NBS-encoding genes were grouped into 6 distinct categories. Phylogenetic analysis grouped these genes into 3 classes: TIR, CC (I), and CC (II). Chromosome location analysis revealed that the distribution of NBS-encoding genes in chromosomes was uneven, with a number ranging from 1 to 34. Multiple stress-related regulatory elements were detected in the promoters, and the NBS-encoding genes’ expression profiles under biotic and abiotic stresses were obtained. According to the bioinformatics analysis, 9 genes were selected for RT-qPCR analysis. The results revealed that <i>IbNBS75</i>, <i>IbNBS219</i>, and <i>IbNBS256</i> respond to stem nematode infection; <i>Ib­NBS240</i>, <i>IbNBS90</i>, and <i>IbNBS80</i> respond to cold stress, while <i>IbNBS208</i>, <i>IbNBS71</i>, and <i>IbNBS159</i> respond to 30% PEG treatment. We hope these results will provide new insights into the evolution of NBS-encoding genes in the sweetpotato genome and contribute to the molecular breeding of sweetpotato in the future.

Sign in / Sign up

Export Citation Format

Share Document