Calcium-induced lipid phase separations and interactions of phosphatidylcholine/anionic phospholipid vesicles. Fluorescence studies using carbazole-labeled and brominated phospholipids

Biochemistry ◽  
1990 ◽  
Vol 29 (12) ◽  
pp. 2930-2938 ◽  
Author(s):  
John R. Silvius
Keyword(s):  
Phospholipid Vesicles ◽  
Lipid Phase ◽  
Anionic Phospholipid ◽  
Phase Separations ◽  
Fluorescence Studies

Lipid phase separations and protein distribution in membranes

1974 ◽  
Vol 2 (5-6) ◽  
pp. 609-616 ◽  
Author(s):  
W. Kleemann ◽  
C. W. M. Grant ◽  
H. M. McConnell
Keyword(s):  
Lipid Phase ◽  
Protein Distribution ◽  
Phase Separations

scholarly journals The K-Segment of Maize DHN1 Mediates Binding to Anionic Phospholipid Vesicles and Concomitant Structural Changes

2009 ◽  
Vol 150 (3) ◽  
pp. 1503-1514 ◽  
Author(s):  
Myong-Chul Koag ◽  
Stephan Wilkens ◽  
Raymond D. Fenton ◽  
Josh Resnik ◽  
Evanly Vo ◽  
...  
Keyword(s):  
Structural Changes ◽  
Phospholipid Vesicles ◽  
Anionic Phospholipid

scholarly journals Reconstitution of cytochrome c oxidase in phospholipid vesicles containing polyvinylic polymers

1989 ◽  
Vol 257 (3) ◽  
pp. 783-787 ◽  
Author(s):  
P Sarti ◽  
G Antonini ◽  
F Malatesta ◽  
B Vallone ◽  
S Villaschi ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Functional Characterization ◽  
Respiratory Control ◽  
Phospholipid Vesicles ◽  
Lipid Phase ◽  
Protein Orientation ◽  
Highly Hydrophobic ◽  
Polymer Interaction

Cytochrome c oxidase was reconstituted in phospholipid vesicles in the presence of highly hydrophobic poly(vinyl alkanoate) polymers. Electron-microscopy observations demonstrated that polymer interaction with the lipid phase induces vesicles to adopt smaller diameters than those typical of standard proteoliposomes. Functional characterization of these polymer-proteoliposome structures indicates that the reconstitution of the enzyme proceeds efficiently without causing either scrambling of the protein orientation in the membrane or loss of respiratory control. A clear dependence of respiratory control ratio on vesicle size was also demonstrated, which is in agreement with a previous model proposed for control of activity of cytochrome c oxidase vesicles [Brunori, Sarti, Colosimo, Antonini, Malatesta, Jones & Wilson (1985) EMBO J. 4, 2365-2368].

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