Molecular modeling of human complement component C4 and its fragments by x-ray and neutron solution scattering

Biochemistry ◽  
1990 ◽  
Vol 29 (5) ◽  
pp. 1167-1175 ◽  
Author(s):  
Stephen J. Perkins ◽  
Adam S. Nealis ◽  
Robert B. Sim
Keyword(s):  
Molecular Modeling ◽  
Complement Component ◽  
Human Complement ◽  
X Ray ◽  
Human Complement Component

scholarly journals Unusual ultrastructure of complement-component-C4b-binding protein of human complement by synchrotron X-ray scattering and hydrodynamic analysis

1986 ◽  
Vol 233 (3) ◽  
pp. 799-807 ◽  
Author(s):  
S J Perkins ◽  
L P Chung ◽  
K B M Reid
Keyword(s):  
Electron Microscopy ◽  
Binding Protein ◽  
Complement Component ◽  
Human Complement ◽  
Hydrodynamic Analysis ◽  
X Ray ◽  
X Ray Scattering ◽  
Primary Amino ◽  
Human Complement Component ◽  
Ray Scattering

Solution X-ray-scattering experiments with the use of synchrotron radiation on the human complement-component-C4b-binding protein showed that its RG is 13 nm and that its Mr is 550,000. From the known primary amino acid sequence and estimated carbohydrate content, C4b-binding protein is inferred to have a total of 7.4 +/- 1 subunits. Heptameric computer models for C4b-binding protein were based on the X-ray-scattering curve to a resolution of 6.4 nm, and literature values for sedimentation coefficients and electron-microscopy images. The macromolecule was represented by a bundle of seven arms held together at the C-terminal end and spaced out by a base containing 23% of C4b-binding protein by volume. If the overall length of each arm is assumed to be 33 nm as seen in electron microscopy, the solution data indicate an average arm-axis angle of 5-10 degrees. The seven arms of C4b-binding protein are found to be close together, in distinction to the splayed-out images seen in electron micrographs.

X-ray Crystal Structure of the C4d Fragment of Human Complement Component C4

2002 ◽  
Vol 322 (5) ◽  
pp. 1103-1115 ◽  
Author(s):  
Jean M.H. van den Elsen ◽  
Alberto Martin ◽  
Veronica Wong ◽  
Liliana Clemenza ◽  
David R. Rose ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Complement Component ◽  
Human Complement ◽  
X Ray ◽  
Human Complement Component

scholarly journals The structure of human complement component C7 and the C5b-7 complex.

1988 ◽  
Vol 263 (1) ◽  
pp. 549-560
Author(s):  
R G DiScipio ◽  
D N Chakravarti ◽  
H J Muller-Eberhard ◽  
G H Fey
Keyword(s):  
Complement Component ◽  
Human Complement ◽  
Human Complement Component

scholarly journals Bgl II polymorphic site upstream to the human complement component C4A gene

1987 ◽  
Vol 15 (5) ◽  
pp. 2395-2395 ◽  
Author(s):  
A. Palsdottir ◽  
R. Fossdal ◽  
O. Jensson ◽  
A. Amason
Keyword(s):  
Complement Component ◽  
Polymorphic Site ◽  
Human Complement ◽  
Human Complement Component
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