Effect of monoclonal antibody binding on .alpha.-.beta..gamma. subunit interactions in the rod outer segment G protein, Gt

Biochemistry ◽  
1989 ◽  
Vol 28 (25) ◽  
pp. 9873-9880 ◽  
Author(s):  
Maria R. Mazzoni ◽  
Heidi E. Hamm
Keyword(s):  
Monoclonal Antibody ◽  
G Protein ◽  
Outer Segment ◽  
Antibody Binding ◽  
Subunit Interactions ◽  
Gamma Subunit ◽  
Rod Outer Segment ◽  
Alpha Beta ◽  
Monoclonal Antibody Binding

scholarly journals The effect of the γ-subunit of the cyclic GMP phosphodiesterase of bovine and frog (Rana catesbiana) retinal rod outer segments on the kinetic parameters of the enzyme

1990 ◽  
Vol 265 (3) ◽  
pp. 655-658 ◽  
Author(s):  
M M Whalen ◽  
M W Bitensky ◽  
D J Takemoto
Keyword(s):  
Catalytic Activity ◽  
Cyclic Gmp ◽  
Active Sites ◽  
Outer Segment ◽  
Maximal Activity ◽  
Inhibitory Effect ◽  
Gamma Subunit ◽  
Rod Outer Segment ◽  
Gamma Subunits ◽  
Alpha Beta

Rod-outer-segment cyclic GMP phosphodiesterase (PDE) (subunit composition alpha beta gamma 2) contains catalytic activity in alpha beta. The gamma-subunits are inhibitors. Removal of the gamma-subunits increases Vmax. without affecting the Km. The inhibitory effect of a single gamma-subunit (alpha beta gamma) on the Vmax. of alpha beta is much greater in bovine than in frog (Rana catesbiana) PDE. Bovine PDE in the alpha beta gamma 2 state has a Vmax. that is 2.6 +/- 0.4% of the Vmax. of alpha beta. The removal of one gamma-subunit to give alpha beta gamma results in a Vmax. 5.2 +/- 1% of that for maximal activity. Frog alpha beta gamma 2 has a Vmax. 10.8 +/- 2%, and alpha beta gamma has a Vmax. 50 +/- 18%, of the Vmax. of alpha beta. These data suggest that a single gamma-subunit can inhibit the catalytic activity of active sites on both alpha- and beta-subunits in bovine, but not in frog, rod-outer-segment PDE.

IgE and monoclonal antibody binding by the mite allergen Der p 7

1996 ◽  
Vol 26 (3) ◽  
pp. 308-315 ◽  
Author(s):  
H.-D. SHEN ◽  
K. Y. CHUA ◽  
W. L. LIN ◽  
H. L. CHEN ◽  
K.-H. HSIEH ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Mite Allergen ◽  
Antibody Binding ◽  
Monoclonal Antibody Binding

Enhancing Effect of C1q on IgG Monoclonal Antibody Binding to Hapten

1990 ◽  
Vol 91 (1) ◽  
pp. 103-107 ◽  
Author(s):  
Atsuo Hamada ◽  
Naohiro Watanabe ◽  
Takachika Azuma ◽  
Akio Kobayashi
Keyword(s):  
Monoclonal Antibody ◽  
Antibody Binding ◽  
Enhancing Effect ◽  
Monoclonal Antibody Binding
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