Activation of superoxide radical anion generating oxidase of bovine neutrophils in a cell-free system. Interaction of a cytosolic factor with the plasma membrane and control by G nucleotides

Biochemistry ◽  
1989 ◽  
Vol 28 (17) ◽  
pp. 7116-7123 ◽  
Author(s):  
Erzsebet Ligeti ◽  
Marianne Tardif ◽  
Pierre V. Vignais
Keyword(s):  
Plasma Membrane ◽  
Radical Anion ◽  
Superoxide Radical ◽  
Free System ◽  
Cell Free System ◽  
Superoxide Radical Anion ◽  
A Cell ◽  
And Control ◽  
Bovine Neutrophils ◽  
Cytosolic Factor

scholarly journals Calcium Fluxes across the Plasma Membrane of Commelina communis L. Assayed in a Cell-Free System

1990 ◽  
Vol 93 (3) ◽  
pp. 940-947 ◽  
Author(s):  
Bettina Siebers ◽  
Peter Gräf ◽  
Elmar W. Weiler
Keyword(s):  
Plasma Membrane ◽  
Free System ◽  
Calcium Fluxes ◽  
Cell Free System ◽  
Commelina Communis ◽  
A Cell

Effect of Keigai-Rengyo-To, a Japanese Kampo Medicine, on Neutrophil Functions: A Possible Mechanism of Action of Keigai-Rengyo-To in Acne

1997 ◽  
Vol 25 (5) ◽  
pp. 255-265 ◽  
Author(s):  
H Akamatsu ◽  
Y Asada ◽  
T Horio
Keyword(s):  
Calcium Concentration ◽  
Superoxide Radical ◽  
Clinical Effectiveness ◽  
Human Neutrophils ◽  
Dependent Manner ◽  
Neutrophil Chemotaxis ◽  
Free System ◽  
Superoxide Radical Anion ◽  
A Cell ◽  
Dose Dependent

On the basis of recent reports that keigai-rengyo-to (TJ-50), an oral Japanese Kampo (herb) medicine, is clinically effective in treating acne, and that tetracyclines are effective against acne by acting directly as an antioxidant on infiltrated neutrophils, we investigated the effect of TJ-50 on the generation of reactive oxygen species (ROS), using human neutrophils and a cell-free, xanthine-xanthine oxidase system. The species investigated were superoxide radical anion (O2-), hydrogen peroxide (H2O2), and hydroxyl radical (OH·). In addition, neutrophil chemotaxis, phagocytosis and calcium concentration, [Ca2+]i in neutrophils were also assessed. TJ-50 significantly decreased neutrophil-generated O2-, H2O2 and OH· in a dose-dependent manner. Three kinds of ROS generated in the cell-free system were also reduced in the presence of TJ-50. On the other hand, the medicine did not markedly affect neutrophil chemotaxis, phagocytosis or [Ca2+]i in neutrophils. Our results indicate that the clinical effectiveness of TJ-50 in the treatment of acne may be due partly to its antioxidant action on infiltrated neutrophils.

scholarly journals Effect of detergents on sterol synthesis in a cell-free system of yeast

1982 ◽  
Vol 23 (6) ◽  
pp. 803-810
Author(s):  
S Hata ◽  
T Nishino ◽  
N Ariga ◽  
H Katsuki
Keyword(s):  
Sterol Synthesis ◽  
Free System ◽  
Cell Free System ◽  
A Cell
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