Calcium dependence of the distance between Cys-98 of troponin C and Cys-133 of troponin I in the ternary troponin complex. Resonance energy transfer measurements

Biochemistry ◽  
1989 ◽  
Vol 28 (14) ◽  
pp. 5902-5908 ◽  
Author(s):  
Terence Tao ◽  
Elizabeth Gowell ◽  
Gale M. Strasburg ◽  
John Gergely ◽  
Paul C. Leavis
Keyword(s):  
Energy Transfer ◽  
Troponin I ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Troponin C ◽  
Calcium Dependence ◽  
Troponin Complex

Aptamer-based rolling circle amplification coupled with graphene oxide-based fluorescence resonance energy transfer for sensitive detection of cardiac troponin I

2018 ◽  
Vol 10 (15) ◽  
pp. 1767-1773 ◽  
Author(s):  
Yu Li ◽  
Weiqiang Dai ◽  
Xuefei Lv ◽  
Yulin Deng
Keyword(s):  
Graphene Oxide ◽  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Troponin I ◽  
Rolling Circle Amplification ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Protein Detection ◽  
Rolling Circle ◽  
Fluorescence Resonance

Combining the high affinity and specificity of an aptamer for a protein, a highly sensitive strategy for protein detection was developed based on rolling circle amplification (RCA) and graphene oxide based fluorescence resonance energy transfer (FRET).

A wavelength-resolved electrochemiluminescence resonance energy transfer ratiometric immunosensor for detection of cardiac troponin I

The Analyst ◽  
2019 ◽  
Vol 144 (22) ◽  
pp. 6554-6560 ◽  
Author(s):  
Liping Zhu ◽  
Jing Ye ◽  
Mengxia Yan ◽  
Qiuju Zhu ◽  
Xiurong Yang
Keyword(s):  
Energy Transfer ◽  
Cardiac Troponin ◽  
Troponin I ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Cardiac Troponin I ◽  
First Time

A wavelength-resolved electrochemiluminescence resonance energy transfer ratiometric immunosensor from Au-g-C3N4 to Au NCs has been constructed for the first time.

Quantum Dot Bioconjugates as Energy Donors in Fluorescence Resonance Energy Transfer Assays

2003 ◽  
Vol 773 ◽  
Author(s):  
Aaron R. Clapp ◽  
Igor L. Medintz ◽  
J. Matthew Mauro ◽  
Hedi Mattoussi
Keyword(s):  
Energy Transfer ◽  
Quantum Dot ◽  
Organic Dyes ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Genetically Engineered ◽  
Molar Ratio ◽  
Binding Assays ◽  
Specific Sequence ◽  
Donor Acceptor

AbstractLuminescent CdSe-ZnS core-shell quantum dot (QD) bioconjugates were used as energy donors in fluorescent resonance energy transfer (FRET) binding assays. The QDs were coated with saturating amounts of genetically engineered maltose binding protein (MBP) using a noncovalent immobilization process, and Cy3 organic dyes covalently attached at a specific sequence to MBP were used as energy acceptor molecules. Energy transfer efficiency was measured as a function of the MBP-Cy3/QD molar ratio for two different donor fluorescence emissions (different QD core sizes). Apparent donor-acceptor distances were determined from these FRET studies, and the measured distances are consistent with QD-protein conjugate dimensions previously determined from structural studies.

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