Primary structure of the cAMP-dependent phosphorylation site of the plasma membrane calcium pump

Biochemistry ◽  
1989 ◽  
Vol 28 (10) ◽  
pp. 4253-4258 ◽  
Author(s):  
Peter H. James ◽  
Martin Pruschy ◽  
Thomas E. Vorherr ◽  
John T. Penniston ◽  
Ernesto Carafoli
Keyword(s):  
Plasma Membrane ◽  
Primary Structure ◽  
Phosphorylation Site ◽  
Calcium Pump ◽  
Plasma Membrane Calcium Pump

Positive charge modifications alter the ability of XIP to inhibit the plasma membrane calcium pump

1996 ◽  
Vol 271 (3) ◽  
pp. C736-C741 ◽  
Author(s):  
W. Xu ◽  
C. Gatto ◽  
M. A. Milanick
Keyword(s):  
Plasma Membrane ◽  
Positive Charge ◽  
Calcium Pump ◽  
Inhibitory Peptide ◽  
Ca Pump ◽  
Contact Points ◽  
Plasma Membrane Calcium Pump ◽  
Peptide Analogues ◽  
Positively Charged

Exchange inhibitory peptide (XIP; RRLLFYKYVYKRYRAGKQRG) is the shortest peptide that inhibits the plasma membrane Ca pump at high Ca (A. Enyedi, T. Vorherr, P. James, D. J. McCormick, A. G. Filoteo, E. Carafoli, and J. T. Penniston, J. Biol. Chem. 264: 12313-12321, 1989). Sulfosuccinimidyl acetate (SNA)-modified XIP does not inhibit the Ca pump; SNA neutralizes the positive charge on Lys at positions 7, 11, and 17. Peptide 2CK-XIP (RRLLFYRYVYRCYCAGRQKG) inhibits the pump, but the iodoacetamido-modified peptide does not inhibit. Three peptide analogues, in which 7, 11, and 17 were Ala, Cys, or Lys, inhibited about as well as XIP. SNA modification of these analogues (each with 1 Lys) did not inhibit. SNA modification of 2CK-XIP results in a peptide that does not inhibit; thus position 19 is important. Our results suggest that it is critical that position 19 be positively charged, that positions 7, 11, and 17 are important contact points between XIP and the Ca pump (with at least one positively charged), and that, whereas it is not essential that residues 12 and 14 be positive, they cannot be negative.

scholarly journals Insulin Protects Pancreatic Acinar Cells from Cytosolic Calcium Overload and Inhibition of Plasma Membrane Calcium Pump

2011 ◽  
Vol 287 (3) ◽  
pp. 1823-1836 ◽  
Author(s):  
Parini Mankad ◽  
Andrew James ◽  
Ajith K. Siriwardena ◽  
Austin C. Elliott ◽  
Jason I. E. Bruce
Keyword(s):  
Plasma Membrane ◽  
Acinar Cells ◽  
Cytosolic Calcium ◽  
Calcium Overload ◽  
Calcium Pump ◽  
Pancreatic Acinar Cells ◽  
Plasma Membrane Calcium Pump
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