Interactions of oleic acid with liver fatty acid binding protein: a carbon-13 NMR study [Erratum to document cited in CA108(7):51580v]

Biochemistry ◽  
1989 ◽  
Vol 28 (8) ◽  
pp. 3628-3628 ◽  
Author(s):  
David P. Cistola ◽  
Mary T. Walsh ◽  
Ronald P. Corey ◽  
James A. Hamilton ◽  
Peter Brecher
Keyword(s):  
Oleic Acid ◽  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Protein A ◽  
Liver Fatty Acid ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Nmr Study

Interactions of oleic acid with liver fatty acid binding protein: a carbon-13 NMR study

Biochemistry ◽  
1988 ◽  
Vol 27 (2) ◽  
pp. 711-717 ◽  
Author(s):  
David P. Cistola ◽  
Mary T. Walsh ◽  
Ronald P. Corey ◽  
James A. Hamilton ◽  
Peter Brecher
Keyword(s):  
Oleic Acid ◽  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Protein A ◽  
Liver Fatty Acid ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Nmr Study

scholarly journals Liver fatty acid binding protein: A potential urinary and tissue biomarker for lupus nephritis

2017 ◽  
Vol 39 (4) ◽  
pp. 217-221 ◽  
Author(s):  
Soha Eldessouki Ibrahim ◽  
Nashwa Aly Morshedy ◽  
Marwa M. Shakweer ◽  
Naglaa Farouk
Keyword(s):  
Fatty Acid ◽  
Lupus Nephritis ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Protein A ◽  
Liver Fatty Acid ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Tissue Biomarker

scholarly journals The binding of lysophospholipids to rat liver fatty acid-binding protein and albumin

1994 ◽  
Vol 301 (3) ◽  
pp. 801-806 ◽  
Author(s):  
A E Thumser ◽  
J E Voysey ◽  
D C Wilton
Keyword(s):  
Oleic Acid ◽  
Fatty Acid ◽  
Rat Liver ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Liver Fatty Acid ◽  
Fatty Acid Binding ◽  
Triacylglycerol Lipase ◽  
Acid Binding Protein ◽  
High Affinity

The binding of lysophospholipids to rat liver fatty acid-binding protein (FABP) and to BSA and human serum albumin was investigated by using competitive displacement fluorescence assays by monitoring the displacement of the fluorescent fatty acid probe 11-(dansylamino)undecanoic acid (DAUDA). In addition, direct binding assays using changes in tryptophan fluorescence were possible with albumin. Liver FABP was able to bind a range of lysophospholipids, oleoyl-lysophosphatidic acid (lysoPA), oleoyl-lysophosphatidylcholine (lysoPC), oleoyl-lysophosphatidylethanolamine (lysoPE) and oleoyl-lysophosphatidylglycerol, with similar affinity and a Kd of about 1 microM. Liver FABP was also able to bind lysophospholipids generated by the action of phospholipase A2 or phospholipase A1 (triacylglycerol lipase) on phospholipid vesicles. A possible physiological role for liver FABP in lysophospholipid metabolism within the cell is discussed. Albumin was shown to bind lysoPA with higher affinity than either lysoPC or lysoPE, and the initial minimal DAUDA displacement by lysoPA indicated that lysoPA was binding to the primary high-affinity fatty acid-binding sites on albumin and that, like oleic acid, about 3 mol of ligand/mol was bound to these sites. Kd values in the microM range were indicated for lysoPC and lysoPE, whereas, by comparison with oleic acid, the Kd for lysoPA was significantly lower and high-affinity binding in the nM range was indicated. Overall, the data suggest that, because of structural similarity, lysoPA binds to albumin in a similar manner to long-chain fatty acids.

Ferriheme and ferroheme are isosteric inhibitors of fatty acid binding to rat liver fatty acid binding protein

1996 ◽  
Vol 74 (2) ◽  
pp. 249-255 ◽  
Author(s):  
John M. Stewart ◽  
Gordon W. Slysz ◽  
Mary Anne Pritting ◽  
Ursula Muller-Eberhard
Keyword(s):  
Oleic Acid ◽  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Water Soluble ◽  
Reduced Form ◽  
Liver Fatty Acid ◽  
Fatty Acid Binding ◽  
Diffusional Flux ◽  
Acid Binding Protein

In addition to fatty acids, liver fatty acid binding protein (L-FABP) also interacts with ferriheme, which it binds with an affinity approximately one order of magnitude greater than that for oleic acid. We have, therefore, examined the effect of ferroheme and ferriheme on the binding of oleate to rat L-FABP, also called heme-binding protein. Both oxidation states of heme behaved as isosteric inhibitors for the binding of the fatty acid confirming a common binding site. The reduced form of heme (Fe(II)) is a threefold better competitor of oleate binding than ferriheme. To show whether the diffusion of heme would be affected by the presence of the binding protein, we measured the effect of the fatty acid binding protein on the diffusional flux of a water-soluble heme derivative, iron-deuteroporphyrin. The diffusional flux of iron-deuteroporphyrin did not change in the presence of the protein. This suggested that the binding affinity of fatty acid binding protein for iron-deuteroporphyrin is too great to allow rapid equilibrium between bound and unbound ligand across the system in an appropriate time frame.Key words: fatty acid binding protein, heme, diffusion, liver, oleic acid.

Biochemical and Histochemical Studies of Liver Fatty Acid Binding Protein in Alcohol-Treated Rats.

1993 ◽  
Vol 14 (3) ◽  
pp. 171-181
Author(s):  
Shigeya WATANABE ◽  
Yoshio WAKATSUKI ◽  
Hideyuki YOSHIOKA ◽  
Masami INADA ◽  
Teruo ONO ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Liver Fatty Acid ◽  
Fatty Acid Binding ◽  
Acid Binding Protein

scholarly journals Intracellular sterol distribution in transfected mouse L-cell fibroblasts expressing rat liver fatty acid-binding protein.

1991 ◽  
Vol 266 (9) ◽  
pp. 5486-5496
Author(s):  
J R Jefferson ◽  
J P Slotte ◽  
G Nemecz ◽  
A Pastuszyn ◽  
T J Scallen ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Rat Liver ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Liver Fatty Acid ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
L Cell
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