Effects of single-base bulges on intercalator binding to small RNA and DNA hairpins and a ribosomal RNA fragment

Biochemistry ◽  
1989 ◽  
Vol 28 (4) ◽  
pp. 1892-1897 ◽  
Author(s):  
Susan A. White ◽  
David E. Draper
Keyword(s):  
Ribosomal Rna ◽  
Small Rna ◽  
Dna Hairpins ◽  
Single Base ◽  
Rna And Dna

In vitro synthesis of 16S ribosomal RNA containing single base changes and assembly into a functional 30S ribosome

Biochemistry ◽  
1987 ◽  
Vol 26 (8) ◽  
pp. 2353-2364 ◽  
Author(s):  
W. Krzyzosiak ◽  
R. Denman ◽  
K. Nurse ◽  
W. Hellmann ◽  
M. Boublik ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
16S Ribosomal Rna ◽  
In Vitro Synthesis ◽  
Single Base

DNA Hairpins Containing a Diaminostilbene Derivative as a Photoinduced Electron Donor for Probing the Effects of Single-Base Mismatches on Excess Electron Transfer in DNA

2009 ◽  
Vol 11 (4) ◽  
pp. 927-930 ◽  
Author(s):  
Takeo Ito ◽  
Aiko Hayashi ◽  
Akiko Kondo ◽  
Tsukasa Uchida ◽  
Kazuhito Tanabe ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Electron Donor ◽  
Excess Electron ◽  
Dna Hairpins ◽  
Single Base

The Effect of Base Sequence on the Stability of RNA and DNA Single Base Bulges

Biochemistry ◽  
1999 ◽  
Vol 38 (48) ◽  
pp. 15986-15993 ◽  
Author(s):  
Jian Zhu ◽  
Roger M. Wartell
Keyword(s):  
Base Sequence ◽  
Single Base ◽  
The Stability ◽  
Rna And Dna

Detection of Mycobacterium tuberculosis in Paraffin-Embedded Pleural Biopsy Specimens by Commercial Ribosomal RNA and DNA Amplification Kits

CHEST Journal ◽  
2000 ◽  
Vol 118 (3) ◽  
pp. 648-655 ◽  
Author(s):  
Juan Ruiz-Manzano ◽  
Joxe-Mari Manterola ◽  
Fredy Gamboa ◽  
Ana Calatrava ◽  
Eduardo Monsó ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Ribosomal Rna ◽  
Dna Amplification ◽  
Pleural Biopsy ◽  
Biopsy Specimens ◽  
Rna And Dna

scholarly journals Changes in the patterns of synthesis of ribonucleic acid species in immature rat uterus in response to oestradiol-17β

1969 ◽  
Vol 112 (5) ◽  
pp. 563-569 ◽  
Author(s):  
R J Billing ◽  
B Barbiroli ◽  
R. M. S. Smellie
Keyword(s):  
Ribosomal Rna ◽  
Hormone Treatment ◽  
Transfer Rna ◽  
Time Intervals ◽  
Rat Uterus ◽  
Immature Rat ◽  
Oestradiol Treatment ◽  
Wet Weight ◽  
Major Increase ◽  
Rna And Dna

1. After treatment of immature rats with diethylstilboestrol, the wet weight and RNA content of uterine tissue increased rapidly, reaching a peak at 40hr. After an initial lag of a few hours, the acid-soluble ribose and protein contents also rose to maxima at 40hr. No increase in DNA content occurred until at least 24hr. after treatment. 2. The RNA from immature rat uterus isolated at various times up to 6hr. after administration of oestradiol-17β was labelled by injecting [3H]uridine and [3H]guanosine intraperitoneally 30min. before the animals were killed. It was fractionated on columns of kieselguhr coated with methylated serum albumin and the radioactivity in fractions corresponding to transfer RNA, 7s RNA, ribosomal RNA, Q1-RNA, Q2-RNA and DNA-like RNA was determined. 3. The radioactivity of the whole RNA increased steadily for 6hr. after hormone treatment. The earliest changes occurred in the Q1-RNA (ribosomal RNA precursor), whereas at longer time-intervals the radioactivity of the ribosomal RNA, 7s RNA and transfer RNA increased by four- to five-fold. The radioactivity of the DNA-like RNA increased by about 50%, but only at the longer time-intervals. 4. It is concluded that one of the earliest changes in response to oestradiol treatment is a major increase in synthesis of ribosomal RNA followed later by a similar increase in synthesis of transfer RNA and by a much smaller increase in synthesis of DNA-like RNA. The change in synthesis of ribosomal RNA in immature rat uterus may represent one of the most important responses to oestradiol treatment.

scholarly journals Effects of individual base-pairs on in vivo target search and destruction kinetics of bacterial small RNA

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Anustup Poddar ◽  
Muhammad S. Azam ◽  
Tunc Kayikcioglu ◽  
Maksym Bobrovskyy ◽  
Jichuan Zhang ◽  
...  
Keyword(s):  
Small Rna ◽  
Base Pair ◽  
High Throughput Sequencing ◽  
Base Pairing ◽  
Target Search ◽  
Single Base ◽  
Base Pair Mismatch ◽  
Single Base Pair ◽  
Bacterial Small Rna

AbstractBase-pairing interactions mediate many intermolecular target recognition events. Even a single base-pair mismatch can cause a substantial difference in activity but how such changes influence the target search kinetics in vivo is unknown. Here, we use high-throughput sequencing and quantitative super-resolution imaging to probe the mutants of bacterial small RNA, SgrS, and their regulation of ptsG mRNA target. Mutations that disrupt binding of a chaperone protein, Hfq, and are distal to the mRNA annealing region still decrease the rate of target association, kon, and increase the dissociation rate, koff, showing that Hfq directly facilitates sRNA–mRNA annealing in vivo. Single base-pair mismatches in the annealing region reduce kon by 24–31% and increase koff by 14–25%, extending the time it takes to find and destroy the target by about a third. The effects of disrupting contiguous base-pairing are much more modest than that expected from thermodynamics, suggesting that Hfq buffers base-pair disruptions.

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