Nanosecond dynamics of horse heart apocytochrome c in aqueous solution as studied by time-resolved fluorescence of the single tryptophan residue (Trp-59)

Biochemistry ◽  
1988 ◽  
Vol 27 (24) ◽  
pp. 8752-8761 ◽  
Author(s):  
Michel Vincent ◽  
Jean Claude Brochon ◽  
Fabienne Merola ◽  
Wilco Jordi ◽  
Jacques Gallay
Keyword(s):  
Aqueous Solution ◽  
Tryptophan Residue ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Apocytochrome C ◽  
Nanosecond Dynamics

scholarly journals Nanosecond Dynamics of Calmodulin and Ribosome-Bound Nascent Chains Studied by Time-Resolved Fluorescence Anisotropy

2014 ◽  
Vol 106 (2) ◽  
pp. 670a-671a
Author(s):  
Daryan Kempe ◽  
Paraskevas Lamprou ◽  
Alexandros Katranidis ◽  
Georg Büldt ◽  
Jörg Fitter
Keyword(s):  
Fluorescence Anisotropy ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Nanosecond Dynamics

Local Environment of Surface-Polyelectrolyte-Bound DNA Oligomers

2000 ◽  
Vol 651 ◽  
Author(s):  
Sangmin Jeon ◽  
Sung Chul Bae ◽  
Jiang John Zhao ◽  
Steve Granick
Keyword(s):  
Aqueous Solution ◽  
Salt Concentration ◽  
Fluorescence Anisotropy ◽  
Local Environment ◽  
Time Resolved Fluorescence ◽  
Surface Complexes ◽  
Dna Oligomers ◽  
Time Resolved ◽  
Two Photon ◽  
Anisotropy Decay

AbstractTwo-photon time-resolved fluorescence anisotropy methods were used to study the dynamical environment when fluorescent-labelled DNA oligomers (labelled with FAM, 6-fluorescein-6-carboxamido hexanoate) formed surface complexes with quaternized polyvinylpyridine (QPVP) cationic layers on a glass surface. We compared the anisotropy decay of DNA in bulk aqueous solution, DNA adsorbed onto QPVP, and QPVP-DNA-QPVP sandwich structures. When DNA was adsorbed onto QPVP, its anisotropy decay was dramatically retarded compared to the bulk, which means it had very slow rotational motion on the surface. Motions slowed down with increasing salt concentration up to a level of 0.1 M NaCl, but mobility began to increase at still higher salt concentration owing to detachment from the surface-immobilizing QPVP layers.

Static and time-resolved fluorescence of fluorescein-labeled dextran dissolved in aqueous solution or sequestered within a sol–gel-derived hydrogel

The Analyst ◽  
1999 ◽  
Vol 124 (3) ◽  
pp. 373-379 ◽  
Author(s):  
Gary A. Baker ◽  
A. Neal Watkins ◽  
Siddharth Pandey ◽  
Frank V. Bright
Keyword(s):  
Aqueous Solution ◽  
Sol Gel ◽  
Time Resolved Fluorescence ◽  
Time Resolved

Picosecond time-resolved fluorescence study of poly vinyl methyl ether aqueous solution

2009 ◽  
Vol 468 (4-6) ◽  
pp. 171-175 ◽  
Author(s):  
Tsutomu Sakamaki ◽  
Tatsuya Fujino ◽  
Haruko Hosoi ◽  
Tahei Tahara ◽  
Takashi Korenaga
Keyword(s):  
Aqueous Solution ◽  
Methyl Ether ◽  
Time Resolved Fluorescence ◽  
Vinyl Methyl ◽  
Fluorescence Study ◽  
Time Resolved ◽  
Vinyl Methyl Ether

Time-Resolved Fluorescence Studies of Hematoporphyrin in Different Solvent Systems

1983 ◽  
Vol 38 (1-2) ◽  
pp. 83-89 ◽  
Author(s):  
A. Andreoni ◽  
R. Cubeddu ◽  
S. De Silvestri ◽  
G. Jori ◽  
P. Laporta ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Organic Solvents ◽  
Slow Component ◽  
Fluorescence Decay ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Fluorescence Studies ◽  
Fluorescence Measurements ◽  
Dimeric Form ◽  
Solvent Systems

Time-resolved fluorescence studies of hematoporphyrin in aqueous solution and in different organic solvents are presented. The| observation of two exponential components in the fluorescence decay in aqueous solution reveals the presence of a monomeric and aggregated (probably dimeric) form of the molecule, as confirmed by absorption and fluorescence measurements. The slow component (~ 15 ns in aqueous solution) and the fast one (~ 3.8 ns in aqueous solution) are attributed to monomers and dimers, respectively. Higher aggregated species of hematoporphyrin are also present, which are essentially devoid of fluorescence properties.

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