The high molecular weight nerve growth factor complex from Mastomys natalensis differs from the murine nerve growth factor complex

Biochemistry ◽  
1988 ◽  
Vol 27 (18) ◽  
pp. 6686-6692 ◽  
Author(s):  
Thomas L. J. Darling ◽  
Margaret Fahnestock
Keyword(s):  
Molecular Weight ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
High Molecular Weight ◽  
Mastomys Natalensis ◽  
Nerve Growth

scholarly journals Regulation of a high molecular weight microtubule-associated protein in PC12 cells by nerve growth factor.

1983 ◽  
Vol 96 (1) ◽  
pp. 76-83 ◽  
Author(s):  
L A Greene ◽  
R K Liem ◽  
M L Shelanski
Keyword(s):  
Molecular Weight ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Neurite Outgrowth ◽  
High Molecular Weight ◽  
Pc12 Cells ◽  
Significant Proportion ◽  
Cross Reactivity ◽  
Nerve Growth ◽  
Sds Page

PC12 rat pheochromocytoma cells respond to nerve growth factor (NGF) protein by shifting from a chromaffin-cell-like phenotype to a neurite-bearing sympathetic-neuron-like phenotype. Comparison of the phosphoprotein patterns of the cells by SDS PAGE after various times of NGF treatment revealed a high molecular weight (Mr greater than or approximately 300,000) band whose relative intensity progressively increased beyond 2 d of NGF exposure. This effect was blocked by inhibitors of RNA synthesis and did not require neurite outgrowth or substrate attachment. The enhancement by NGF occurred in serum-free medium and was not produced by exposure to epidermal growth factor, insulin, dibutyryl cAMP, or dexamethasone. Several different types of experiments indicated that this phosphoprotein corresponds to a high molecular weight (HMW) microtubule-associated protein (MAP). These included cross-reactivity with antiserum against brain HMW MAPs, co-cycling with microtubules and co-assembly with tubulin in the presence of taxol. The affected species also co-migrated in SDS PAGE gels with brain MAP1 and, unlike MAP2, precipitated upon boiling. Studies with [35S]-methionine-labeled PC12 cells indicated that at least a significant proportion of this effect of NGF was due to increased levels of protein rather than to mere enhancement of phosphorylation. On the basis of the apparent effects of MAPs on the formation and stabilization of microtubules and of the importance of microtubules in production and maintenance of neurites, it is proposed that induction of a HMW MAP may be one of the steps in the mechanism whereby NGF promotes neurite outgrowth. Furthermore, these findings may lead to an understanding of the role of MAP1 in the nervous system.

Subunit structure of high molecular weight mouse nerve growth factor

Biochemistry ◽  
1988 ◽  
Vol 27 (18) ◽  
pp. 6675-6681 ◽  
Author(s):  
Michael Young ◽  
Muriel H. Blanchard ◽  
Freida Sessions ◽  
Michael D. P. Boyle
Keyword(s):  
Molecular Weight ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
High Molecular Weight ◽  
Subunit Structure ◽  
Nerve Growth
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