Rotational dynamics of the single tryptophan of porcine pancreatic phospholipase A2, its zymogen, and an enzyme/micelle complex. A steady-state and time-resolved anisotropy study

Biochemistry ◽  
1988 ◽  
Vol 27 (17) ◽  
pp. 6618-6628 ◽  
Author(s):  
Richard D. Ludescher ◽  
Iain D. Johnson ◽  
Johannes J. Volwerk ◽  
Gerard H. De Haas ◽  
Patricia C. Jost ◽  
...  
Keyword(s):  
Steady State ◽  
Phospholipase A2 ◽  
Rotational Dynamics ◽  
Time Resolved ◽  
Pancreatic Phospholipase ◽  
Pancreatic Phospholipase A2

Time-resolved fluoroimmunoassay of human pancreatic phospholipase A2.

1983 ◽  
Vol 29 (10) ◽  
pp. 1777-1780 ◽  
Author(s):  
J U Eskola ◽  
T J Nevalainen ◽  
T N Lövgren
Keyword(s):  
Phospholipase A2 ◽  
Solid Phase ◽  
High Sensitivity ◽  
Fluorometric Detection ◽  
Time Resolved ◽  
Antibody Technique ◽  
Pancreatic Phospholipase ◽  
Pancreatic Phospholipase A2 ◽  
Immunoreactive Phospholipase A2 ◽  
Solid Phase Assay

Abstract We describe an immunofluorometric assay for human pancreatic phospholipase A2 based on time-resolved fluorescence. The labeled antibody technique in combination with the time-resolved 1-s fluorometric detection of the europium label, which essentially eliminates all background fluorescence, resulted in a high sensitivity (20 ng/L) and a wide (5000-fold) linear range. Nonspecific binding was minimized by treating the solid-phase antibody with NaSCN before coating, to remove endogenous antigen, and by immunosorbent purification of the antibody before labeling with europium. This is a one-incubation, multi-site, solid-phase assay on polystyrene microtiter strips, even though a polyclonal antibody was used. As measured by this assay, activity of immunoreactive phospholipase A2 was found to be above normal in sera of patients suffering from acute pancreatitis.

Molecular Characterization of Murine Pancreatic Phospholipase A2

2001 ◽  
Vol 20 (3) ◽  
pp. 149-157 ◽  
Author(s):  
Asim K. Mandal ◽  
Zhongjian Zhang ◽  
Janice Y. Chou ◽  
Drazen Zimonjic ◽  
Catherine L. Keck ◽  
...  
Keyword(s):  
Phospholipase A2 ◽  
Molecular Characterization ◽  
Pancreatic Phospholipase ◽  
Pancreatic Phospholipase A2
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