Involvement of tryptophan residues at the coenzyme A binding site of carbon monoxide dehydrogenase from Clostridium thermoaceticum

Biochemistry ◽  
1988 ◽  
Vol 27 (17) ◽  
pp. 6499-6503 ◽  
Author(s):  
T. Shanmugasundaram ◽  
Ganesh K. Kumar ◽  
Harland G. Wood
Keyword(s):  
Carbon Monoxide ◽  
Binding Site ◽  
Coenzyme A ◽  
Carbon Monoxide Dehydrogenase ◽  
Clostridium Thermoaceticum ◽  
Tryptophan Residues

Mechanism of CO oxidation by carbon monoxide dehydrogenase from Clostridium thermoaceticum and its inhibition by anions

Biochemistry ◽  
1995 ◽  
Vol 34 (24) ◽  
pp. 7879-7888 ◽  
Author(s):  
Javier Seravalli ◽  
Manoj Kumar ◽  
Wei-Ping Lu ◽  
Stephen W. Ragsdale
Keyword(s):  
Carbon Monoxide ◽  
Co Oxidation ◽  
Carbon Monoxide Dehydrogenase ◽  
Clostridium Thermoaceticum

scholarly journals 2,4,6-Trinitrotoluene Reduction by Carbon Monoxide Dehydrogenase from Clostridium thermoaceticum

2000 ◽  
Vol 66 (4) ◽  
pp. 1474-1478 ◽  
Author(s):  
Shouqin Huang ◽  
Paul A. Lindahl ◽  
Chuanyue Wang ◽  
George N. Bennett ◽  
Frederick B. Rudolph ◽  
...  
Keyword(s):  
Carbon Monoxide ◽  
Carbon Monoxide Dehydrogenase ◽  
Clostridium Thermoaceticum ◽  
Co Dehydrogenase ◽  
Rearrangement Product ◽  
Reduction Activity ◽  
Oxidation Reduction ◽  
Reduced Products ◽  
Degradation Activity ◽  
Dominant Isomer

ABSTRACT Purified CO dehydrogenase (CODH) from Clostridium thermoaceticum catalyzed the transformation of 2,4,6-trinitrotoluene (TNT). The intermediates and reduced products of TNT transformation were separated and appear to be identical to the compounds formed by C. acetobutylicum, namely, 2-hydroxylamino-4,6-dinitrotoluene (2HA46DNT), 4-hydroxylamino-2,6-dinitrotoluene (4HA26DNT), 2,4-dihydroxylamino-6-nitrotoluene (24DHANT), and the Bamberger rearrangement product of 2,4-dihydroxylamino-6-nitrotoluene. In the presence of saturating CO, CODH catalyzed the conversion of TNT to two monohydroxylamino derivatives (2HA46DNT and 4HA26DNT), with 4HA26DNT as the dominant isomer. These derivatives were then converted to 24DHANT, which slowly converted to the Bamberger rearrangement product. ApparentKm and k cat values of TNT reduction were 165 ± 43 μM for TNT and 400 ± 94 s−1, respectively. Cyanide, an inhibitor for the CO/CO2 oxidation/reduction activity of CODH, inhibited the TNT degradation activity of CODH.

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