Isotope partitioning in the adenosine 3',5'-monophosphate dependent protein kinase reaction indicates a steady-state random kinetic mechanism

Biochemistry ◽  
1988 ◽  
Vol 27 (13) ◽  
pp. 4795-4799 ◽  
Author(s):  
Cheng Te Kong ◽  
Paul F. Cook
Keyword(s):  
Steady State ◽  
Protein Kinase ◽  
Kinetic Mechanism ◽  
Dependent Protein Kinase ◽  
Kinase Reaction ◽  
Dependent Protein ◽  
Isotope Partitioning

Adenosine cyclic 3',5'-monophosphate dependent protein kinase: kinetic mechanism for the bovine skeletal muscle catalytic subunit

Biochemistry ◽  
1982 ◽  
Vol 21 (23) ◽  
pp. 5794-5799 ◽  
Author(s):  
Paul F. Cook ◽  
Maynard E. Neville ◽  
Kent E. Vrana ◽  
F. Thomas Hartl ◽  
Robert Roskoski
Keyword(s):  
Skeletal Muscle ◽  
Protein Kinase ◽  
Kinetic Mechanism ◽  
Catalytic Subunit ◽  
Dependent Protein Kinase ◽  
Dependent Protein ◽  
Bovine Skeletal Muscle

scholarly journals Identification and regulation of whole-cell chloride currents in airway epithelium.

1989 ◽  
Vol 94 (6) ◽  
pp. 1015-1036 ◽  
Author(s):  
J D McCann ◽  
M Li ◽  
M J Welsh
Keyword(s):  
Steady State ◽  
Protein Kinase ◽  
Patch Clamp ◽  
Apical Membrane ◽  
Patch Clamp Technique ◽  
Dependent Protein Kinase ◽  
Cl Channel ◽  
Camp Dependent Protein Kinase ◽  
Dependent Protein ◽  
Conductive Properties

We used the whole-cell patch-clamp technique to study membrane currents in human airway epithelial cells. The conductive properties, as described by the instantaneous current-voltage relationship, rectified in the outward direction when bathed in symmetrical CsCl solutions. In the presence of Cl concentration gradients, currents reversed near ECl and were not altered significantly by cations. Agents that inhibit the apical membrane Cl conductance inhibited Cl currents. These conductive properties are similar to the conductive properties of the apical membrane Cl channel studied with the single-channel patch-clamp technique. The results suggest that the outwardly rectifying Cl channel is the predominant Cl-conductive pathway in the cell membrane. The steady-state and non-steady-state kinetics indicate that current flows through ion channels that are open at hyperpolarizing voltages and close with depolarization. These Cl currents were regulated by the cAMP-dependent protein kinase: when the catalytic subunit of cAMP-dependent protein kinase was included in the pipette solution, Cl channel current more than doubled. We also found that reducing extracellular osmolarity by 30% increased Cl current, suggesting that cell-swelling stimulated Cl current. Studies of transepithelial Cl transport in cell monolayers suggest that a reduction in solution osmolarity activates the apical Cl channel: reducing extracellular osmolarity stimulated a short-circuit current that was inhibited by Cl-free solution, by mucosal addition of a Cl channel antagonist, and by submucosal addition of a loop diuretic. These results suggest that apical membrane Cl channels may be regulated by cell volume and by the cAMP-dependent protein kinase.

scholarly journals Steady-State Modulation of Voltage-Gated K+ Channels in Rat Arterial Smooth Muscle by Cyclic AMP-Dependent Protein Kinase and Protein Phosphatase 2B

PLoS ONE ◽  
2015 ◽  
Vol 10 (3) ◽  
pp. e0121285 ◽  
Author(s):  
Jennifer L. Brignell ◽  
Matthew D. Perry ◽  
Carl P. Nelson ◽  
Jonathon M. Willets ◽  
R. A. John Challiss ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Steady State ◽  
Protein Kinase ◽  
Cyclic Amp ◽  
Protein Phosphatase ◽  
Dependent Protein Kinase ◽  
K Channels ◽  
Voltage Gated ◽  
Protein Phosphatase 2B ◽  
Dependent Protein
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