Lipid-lipid interactions in reconstituted high-density lipoproteins by deuterium nuclear magnetic resonance

Biochemistry ◽  
1988 ◽  
Vol 27 (12) ◽  
pp. 4491-4500 ◽  
Author(s):  
David B. Fenske ◽  
Yashpal I. Parmar ◽  
W. Dale Treleaven ◽  
Ravinder S. Chana ◽  
Robert J. Cushley
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
High Density ◽  
High Density Lipoproteins ◽  
Lipid Interactions ◽  
Nuclear Magnetic

Phosphorus-31 nuclear magnetic resonance studies on serum low and high density lipoproteins. Effect of paramagnetic ion

Biochemistry ◽  
1975 ◽  
Vol 14 (9) ◽  
pp. 1915-1920 ◽  
Author(s):  
Thomas O. Henderson ◽  
Arthur W. Kruski ◽  
Leonard G. Davis ◽  
Thomas Glonek ◽  
Angelo M. Scanu
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
High Density ◽  
High Density Lipoproteins ◽  
Magnetic Resonance Studies ◽  
Nuclear Magnetic

Quantification of plasma lipoproteins by proton nuclear magnetic resonance spectroscopy

1991 ◽  
Vol 37 (3) ◽  
pp. 377-386 ◽  
Author(s):  
J D Otvos ◽  
E J Jeyarajah ◽  
D W Bennett
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Curve Fitting ◽  
Plasma Lipoproteins ◽  
Proton Nuclear Magnetic Resonance ◽  
High Density Lipoproteins ◽  
Resonance Spectroscopy ◽  
Single Spectrum ◽  
Moderate Magnetic Field ◽  
Nuclear Magnetic

Abstract A new analytical procedure for quantifying plasma lipoproteins by proton nuclear magnetic resonance (NMR) spectroscopy has been developed that potentially offers significant advantages over existing clinical methods used for assessing risk of coronary heart disease. Analysis of a single spectrum of a nonfasting plasma sample, acquired simply and rapidly at moderate magnetic field strength (250 MHz), yields a complete profile of lipoprotein concentrations: chylomicrons and very-low-, low-, and high-density lipoproteins. The method is based on curve-fitting (spectral deconvolution) of the plasma methyl lipid resonance envelope, the amplitude and shape of which depend directly on the amplitudes of the superimposed methyl resonances of the lipoprotein components. A linear least-squares curve-fitting algorithm was developed to efficiently extract the signal amplitudes (concentrations) of the lipoproteins from the plasma spectrum. These signal amplitudes correlate well with lipoprotein concentrations determined by triglyceride and cholesterol measurements.

Relationships between the proton nuclear magnetic resonance properties of plasma lipoproteins and cancer

1991 ◽  
Vol 37 (3) ◽  
pp. 369-376 ◽  
Author(s):  
J D Otvos ◽  
E J Jeyarajah ◽  
L W Hayes ◽  
D S Freedman ◽  
N A Janjan ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Plasma Lipids ◽  
Plasma Lipoproteins ◽  
Proton Nuclear Magnetic Resonance ◽  
High Density Lipoproteins ◽  
Cancer Group ◽  
Lipoprotein Lipid ◽  
The Difference ◽  
Nuclear Magnetic

Abstract We conducted a comprehensive investigation of the origin of nuclear magnetic resonance (NMR) lineshape variability of plasma lipids among healthy individuals and those with cancer. The methyl and methylene resonances of lipid in human plasma, whose linewidths have been reported to correlate with the presence of malignancy, are composed of the overlapping resonances of "mobile" protons from the major lipoproteins (very-low-, low-, and high-density lipoproteins). We tested two hypotheses for the origin of the narrower plasma linewidths observed for cancer patients: (a) malignancy-associated differences in the spectral properties (chemical shift, lineshape) of one or more of the lipoproteins, and (b) differences in the fraction of lipoprotein lipid giving rise to detectable NMR signal. Analysis of the concentrations of lipoprotein lipid and of 500 MHz NMR spectra of the lipoprotein constituents in greater than 100 plasma samples failed to provide support for either hypothesis. Although linewidths were found to be significantly narrower for the cancer group, the difference is entirely attributable to differences in the concentrations of the lipoproteins.

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