Sequence specificity of DNA cleavage by bis(1,10-phenanthroline)copper(I)

Biochemistry ◽  
1988 ◽  
Vol 27 (6) ◽  
pp. 1822-1827 ◽  
Author(s):  
James M. Veal ◽  
Randolph L. Rill
Keyword(s):  
Dna Cleavage ◽  
Sequence Specificity

Conformational properties of topoisomerase II inhibitors and sequence specificity of DNA cleavage

1994 ◽  
Vol 7 (3) ◽  
pp. 227-231 ◽  
Author(s):  
M. Palumbo ◽  
M. Mabilia ◽  
A. Pozzan ◽  
G. Capranico ◽  
S. Tinelli ◽  
...  
Keyword(s):  
Dna Cleavage ◽  
Topoisomerase Ii ◽  
Sequence Specificity ◽  
Topoisomerase Ii Inhibitors ◽  
Conformational Properties

scholarly journals The Need for Speed: Run-On Oligomer Filament Formation Provides Maximum Speed with Maximum Sequestration of Activity

2018 ◽  
Vol 93 (5) ◽  
Author(s):  
Claudia J. Barahona ◽  
L. Emilia Basantes ◽  
Kassidy J. Tompkins ◽  
Desirae M. Heitman ◽  
Barbara I. Chukwu ◽  
...  
Keyword(s):  
Dna Cleavage ◽  
Biological Function ◽  
Enzyme Mechanism ◽  
Maximum Speed ◽  
Host Bacterium ◽  
Sequence Specificity ◽  
Filament Formation ◽  
Dna Sequence Specificity ◽  
Cleavage Activity ◽  
Dna Cleavage Activity

ABSTRACTHere, we investigate an unusual antiviral mechanism developed in the bacteriumStreptomyces griseus. SgrAI is a type II restriction endonuclease that forms run-on oligomer filaments when activated and possesses both accelerated DNA cleavage activity and expanded DNA sequence specificity. Mutations disrupting the run-on oligomer filament eliminate the robust antiphage activity of wild-type SgrAI, and the observation that even relatively modest disruptions completely abolish this anti-viral activity shows that the greater speed imparted by the run-on oligomer filament mechanism is critical to its biological function. Simulations of DNA cleavage by SgrAI uncover the origins of the kinetic advantage of this newly described mechanism of enzyme regulation over more conventional mechanisms, as well as the origin of the sequestering effect responsible for the protection of the host genome against damaging DNA cleavage activity of activated SgrAI.IMPORTANCEThis work is motivated by an interest in understanding the characteristics and advantages of a relatively newly discovered enzyme mechanism involving filament formation. SgrAI is an enzyme responsible for protecting against viral infections in its host bacterium and was one of the first such enzymes shown to utilize such a mechanism. In this work, filament formation by SgrAI is disrupted, and the effects on the speed of the purified enzyme as well as its function in cells are measured. It was found that even small disruptions, which weaken but do not destroy filament formation, eliminate the ability of SgrAI to protect cells from viral infection, its normal biological function. Simulations of enzyme activity were also performed and show how filament formation can greatly speed up an enzyme’s activation compared to that of other known mechanisms, as well as to better localize its action to molecules of interest, such as invading phage DNA.

scholarly journals Nucleotide Sequence Specificity in DNA Cleavage Caused by Dihydropyrazines, a New Type of DNA Strand-Cleaving Agent.

2000 ◽  
Vol 23 (11) ◽  
pp. 1281-1286 ◽  
Author(s):  
Nobuhiro KASHIGE ◽  
Tadatoshi YAMAGUCHI ◽  
Fumio MIAKE ◽  
Kenji WATANABE
Keyword(s):  
Nucleotide Sequence ◽  
Dna Cleavage ◽  
Sequence Specificity ◽  
New Type ◽  
Dna Strand

Conformational Drug Determinants of the Sequence Specificity of Drug-stimulated Topoisomerase II DNA Cleavage

1994 ◽  
Vol 235 (4) ◽  
pp. 1218-1230 ◽  
Author(s):  
Giovanni Capranico ◽  
Manlio Palumbo ◽  
Stella Tinelli ◽  
Massimo Mabilia ◽  
Alfonso Pozzan ◽  
...  
Keyword(s):  
Dna Cleavage ◽  
Topoisomerase Ii ◽  
Sequence Specificity

Sequence Specificity of DNA Cleavage by Micrococcus luteus γ Endonuclease

1985 ◽  
Vol 102 (1) ◽  
pp. 119 ◽  
Author(s):  
Patricia Hentosh ◽  
William D. Henner ◽  
Richard J. Reynolds
Keyword(s):  
Dna Cleavage ◽  
Micrococcus Luteus ◽  
Sequence Specificity
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