Benzylic monooxygenation catalyzed by toluene dioxygenase from Pseudomonas putida

Biochemistry ◽  
1988 ◽  
Vol 27 (4) ◽  
pp. 1360-1367 ◽  
Author(s):  
Lawrence P. Wackett ◽  
Lawrence D. Kwart ◽  
David T. Gibson
Keyword(s):  
Pseudomonas Putida ◽  
Toluene Dioxygenase

Toluene dioxygenase expression correlates with trichloroethylene degradation capacity in Pseudomonas putida F1 cultures

2012 ◽  
Vol 23 (5) ◽  
pp. 683-691 ◽  
Author(s):  
Jianbo Liu ◽  
Takashi Amemiya ◽  
Qing Chang ◽  
Yi Qian ◽  
Kiminori Itoh
Keyword(s):  
Pseudomonas Putida ◽  
Toluene Dioxygenase ◽  
Trichloroethylene Degradation ◽  
Pseudomonas Putida F1

scholarly journals Benzene dioxygenase in Pseudomonas putida. Subunit composition and immuno-cross-reactivity with other aromatic dioxygenases

1987 ◽  
Vol 244 (3) ◽  
pp. 611-616 ◽  
Author(s):  
M Zamanian ◽  
J R Mason
Keyword(s):  
Pseudomonas Putida ◽  
Gel Electrophoresis ◽  
Polyclonal Antibodies ◽  
Polyacrylamide Gel Electrophoresis ◽  
Cross Reactivity ◽  
Polyacrylamide Gel ◽  
Subunit Composition ◽  
Naphthalene Dioxygenase ◽  
Toluene Dioxygenase ◽  
Benzoate Dioxygenase

The terminal oxygenase component of benzene dioxygenase from Pseudomonas putida strain ML2 was shown to contain two subunits, of Mr 54,500 and 23,500, by SDS/polyacrylamide-gel electrophoresis. The native Mr of the terminal oxygenase was estimated to be 168,000 +/- 4000. Polyclonal antibodies raised against each of the subunits cross-reacted with two polypeptides in cell-free extracts from toluene-grown Pseudomonas putida strain N.C.I.B. 11767. The Mr values of these polypeptides were similar to those reported for the subunits from the terminal dioxygenase component of toluene dioxygenase. These polypeptides were present only when this strain was grown on toluene. No cross-reactivity was observed with subunits of the naphthalene dioxygenase or benzoate dioxygenase systems.

scholarly journals Purification and properties of ferredoxinTOL. A component of toluene dioxygenase from Pseudomonas putida F1.

1985 ◽  
Vol 260 (4) ◽  
pp. 2355-2363
Author(s):  
V Subramanian ◽  
T N Liu ◽  
W K Yeh ◽  
C M Serdar ◽  
L P Wackett ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Toluene Dioxygenase ◽  
Purification And Properties ◽  
Pseudomonas Putida F1
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