Thermotropic phase behavior of model membranes composed of phosphatidylcholines containing cis-monounsaturated acyl chain homologs of oleic acid: differential scanning calorimetric and phosphorus-31 NMR spectroscopic studies

Biochemistry ◽  
1988 ◽  
Vol 27 (3) ◽  
pp. 880-887 ◽  
Author(s):  
Ruthven N. A. H. Lewis ◽  
Brian D. Sykes ◽  
Ronald N. McElhaney
Keyword(s):  
Oleic Acid ◽  
Phase Behavior ◽  
Acyl Chain ◽  
Spectroscopic Studies ◽  
Model Membranes ◽  
Differential Scanning Calorimetric

Investigations of Interactions Between Altretamine and Model Membranes: Spectroscopic and Calorimetric Analysis

2019 ◽  
Vol 14 (04) ◽  
pp. 197-215
Author(s):  
D. Bilge ◽  
N. Civelek ◽  
Z. Özçelik Çetinel
Keyword(s):  
Molecular Level ◽  
Acyl Chain ◽  
Spectroscopic Studies ◽  
Model Membranes ◽  
Head Group ◽  
Scanning Calorimetry ◽  
Calorimetric Analysis ◽  
Multilamellar Vesicles ◽  
Calorimetric Studies ◽  
Chain Lengths

Altretamine (ALT) is a Food and Drug Administration (FDA) approved antineoplastic drug particularly used for ovarian cancer. This study examined, at a molecular level, the interactions of the drug with model membranes composed of phospholipids with different acyl chain lengths and head group charges at varied ALT concentrations based on temperature. For this purpose, spectroscopic studies of the liposomes in multilamellar vesicles form were conducted by Fourier transform-infrared spectroscopy (FTIR) and their calorimetric studies were carried out by differential scanning calorimetry (DSC) techniques. The results of the study showed that ALT clearly interacted with lipids and that these interactions were more significant in multilamellar vesicles made up of short chain phospholipids. Moreover, the results suggested that ALT settled into the tail group region, in particular the region that formed the hydrophobic part of lipids, and this effects the whole section of the membranes including glycerol backbones and head groups. This study is expected to contribute, on molecular level, to the studies on the knowledge of the mechanism in cancer, which is still very much a dangerous disease, and its related treatment.

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