Cloning, DNA sequence analysis, and expression in Escherichia coli of the gene for mandelate racemase from Pseudomonas putida

Biochemistry ◽  
1988 ◽  
Vol 27 (2) ◽  
pp. 540-545 ◽  
Author(s):  
Stephen C. Ransom ◽  
John A. Gerlt ◽  
Vincent M. Powers ◽  
George L. Kenyon
Keyword(s):  
Escherichia Coli ◽  
Sequence Analysis ◽  
Pseudomonas Putida ◽  
Dna Sequence ◽  
Dna Sequence Analysis ◽  
Expression In Escherichia Coli ◽  
Mandelate Racemase

Genetic mapping and DNA sequence analysis of mutations in the polA gene of Escherichia coli

1985 ◽  
Vol 186 (2) ◽  
pp. 283-293 ◽  
Author(s):  
Catherine M. Joyce ◽  
David M. Fujii ◽  
Helaina S. Laks ◽  
Cynthia M. Hughes ◽  
Nigel D.F. Grindley
Keyword(s):  
Escherichia Coli ◽  
Sequence Analysis ◽  
Genetic Mapping ◽  
Dna Sequence ◽  
Dna Sequence Analysis

scholarly journals Native Plasmids of Fusobacterium nucleatum: Characterization and Use in Development of Genetic Systems

2000 ◽  
Vol 182 (4) ◽  
pp. 1176-1180 ◽  
Author(s):  
Susan Kinder Haake ◽  
Sean C. Yoder ◽  
Gwynne Attarian ◽  
Kara Podkaminer
Keyword(s):  
Escherichia Coli ◽  
Sequence Analysis ◽  
Dna Sequence ◽  
Fusobacterium Nucleatum ◽  
Dna Sequence Analysis ◽  
Modification System ◽  
Restriction Modification System ◽  
Shuttle Plasmid ◽  
Genetic Systems ◽  
Restriction Modification

ABSTRACT Three native plasmids of Fusobacterium nucleatum were characterized, including DNA sequence analysis of one plasmid, pFN1. A shuttle plasmid, pHS17, capable of transforming Escherichia coli and F. nucleatum ATCC 10953 was constructed with pFN1. pHS17 was stably maintained in the F. nucleatum transformants, and differences in the transformation efficiencies suggested the presence of a restriction-modification system in F. nucleatum.

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