Two histidine residues are essential for ribonuclease T1 activity as is the case for ribonuclease A

Biochemistry ◽  
1987 ◽  
Vol 26 (26) ◽  
pp. 8620-8624 ◽  
Author(s):  
Satoshi Nishikawa ◽  
Hiroshi Morioka ◽  
Hyo J. Kim ◽  
Kayoko Fuchimura ◽  
Toshiki Tanaka ◽  
...  
Keyword(s):  
Ribonuclease A ◽  
Ribonuclease T1 ◽  
Histidine Residues

Contribution of histidine residues to the conformational stability of ribonuclease T1 and mutant Glu-58 .fwdarw. Ala

Biochemistry ◽  
1990 ◽  
Vol 29 (33) ◽  
pp. 7572-7576 ◽  
Author(s):  
Marsha McNutt ◽  
Leisha S. Mullins ◽  
Frank M. Raushel ◽  
C. Nick Pace
Keyword(s):  
Conformational Stability ◽  
Ribonuclease T1 ◽  
Histidine Residues

scholarly journals Localization of the ribonucleotide sites in rat liver mitochondrial deoxyribonucleic acid

1978 ◽  
Vol 169 (1) ◽  
pp. 79-85 ◽  
Author(s):  
D M Lonsdale ◽  
I G Jones
Keyword(s):  
Mitochondrial Dna ◽  
Electron Microscope ◽  
Rat Liver ◽  
Deoxyribonucleic Acid ◽  
Ribonuclease A ◽  
Single Strand ◽  
Ribonuclease H ◽  
Ribonuclease T1 ◽  
Dna Endonuclease ◽  
Calf Thymus

Supercoiled rat liver mitochondrial DNA is relaxed by treatment with ribonucleases A, T1 or H. All the supercoiled mitochondrial DNA is sensitive to ribonuclease H and ribonuclease A, but only 35% of the supercoiled population is sensitive to ribonuclease T1. Removal of the ribonucleotides with calf thymus ribonuclease H, followed by denaturation of the mitochondrial DNA and analysis of the single-strand fragment lengths in the electron microscope, showed that the ribonucleotides were randomly located on both strands of the DNA. Endonuclease-S1 digestion of mitochondrial DNA after removal of the ribonucleotides reveals that no unique fragments are produced and ribonucleotides are randomly distributed with respect to one another. The average number of ribonucleotide sites per molecule was estimated to be between 8 and 13. Two possible mechanisms for the origin of ribonucleotide sites are discussed.

Contribution of the Active Site Histidine Residues of Ribonuclease A to Nucleic Acid Binding†

Biochemistry ◽  
2001 ◽  
Vol 40 (16) ◽  
pp. 4949-4956 ◽  
Author(s):  
Chiwook Park ◽  
L. Wayne Schultz ◽  
Ronald T. Raines
Keyword(s):  
Nucleic Acid ◽  
Active Site ◽  
Ribonuclease A ◽  
Nucleic Acid Binding ◽  
Histidine Residues ◽  
Active Site Histidine

pH dependence of the urea and guanidine hydrochloride denaturation of ribonuclease A and ribonuclease T1

Biochemistry ◽  
1990 ◽  
Vol 29 (10) ◽  
pp. 2564-2572 ◽  
Author(s):  
C. Nick Pace ◽  
Douglas V. Laurents ◽  
James A. Thomson
Keyword(s):  
Ph Dependence ◽  
Guanidine Hydrochloride ◽  
Ribonuclease A ◽  
Ribonuclease T1
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